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SSH1 Antibody

  • 货号：
    CSB-PA837881LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA837881LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA837881LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SSH1 Polyclonal antibody
  • Uniprot No.：
    Q8WYL5
  • 基因名：
  • 别名：
    AW551225 antibody; Coro1c antibody; coronin; actin binding protein 1C antibody; Gm1394 antibody; Gm1395 antibody; hSSH-1L antibody; mSSH 1L antibody; OTTMUSP00000031415 antibody; Protein phosphatase Slingshot homolog 1 antibody; Slingshot 1 antibody; slingshot 1L antibody; slingshot homolog 1 antibody; slingshot like 1 antibody; Slingshot1 antibody; SSH 1 antibody; SSH 1L antibody; SSH-1L antibody; SSH-like protein 1 antibody; Ssh1 antibody; SSH1_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein phosphatase Slingshot homolog 1 protein (928-1049AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SSH1 Antibody (CSB-PA837881LA01HU)，的标记方式是Non-conjugated。对于SSH1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA837881LB01HU SSH1 Antibody, HRP conjugated ELISA
    FITC CSB-PA837881LC01HU SSH1 Antibody, FITC conjugated
    Biotin CSB-PA837881LD01HU SSH1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
  • 基因功能参考文献：
    1. SSH1 binds to gelsolin via actin filaments in the cytosolic fraction. Gelsolin promoted solubilization of actin filaments and SSH1 in cell-free assays and in cultured cells. PMID: 25451266
    2. the N-terminal PH-like domain plays a critical role in F-actin binding and F-actin-mediated activation of the cofilin-phosphatase activity of SSH1 PMID: 27865840
    3. Results reveal that SSH1L is upregulated in a subset of PCs and that the SSH1L/cofilin-1 signal pathway is associated positively in PC with cell migration. PMID: 25684665
    4. s show that viral penetration-induced calcium release facilitated herpes simplex virus type 1 intracellular trafficking through activating slingshot 1 (SSH), a phosphatase regulating actin filament dynamics. PMID: 24670325
    5. The association of IRS4 with SSH1 contributes to localized activation of cofilin in membrane protrusions. PMID: 25100728
    6. Slingshot 1 downregulation in neuronal cells inactivates cofilin to facilitate HSV-1 replication. PMID: 24418541
    7. Results identify a novel frameshift mutation in this gene implicated in disseminated superficial actinic porokeratosis in 4 Chinese families PMID: 23834120
    8. Thus this study provides evidence for a novel role of LIMK1 and SSH-1L in selectively regulating endothelial cell inflammation associated with intravascular coagulation. PMID: 24039253
    9. Our work provides evidence that supports the positive role of SSH1L in the mechanism of stem cell differentiation into cardiomyocyte-like cells. PMID: 23247370
    10. A high expression of SSH-1L in peripheral eosinophils in acute exacerbations of asthma may play a role in the activation and migration of eosinophils. PMID: 21690039
    11. EphA signaling promotes actin-based dendritic spine remodeling through slingshot 1. PMID: 22282498
    12. identified Ser 402 as a new phosphorylation site within the catalytic domain of human slingshot 1 (SSH1). PMID: 21525957
    13. Results show the significance of SSH1L in estrogen-induced osteoblastogenic differentiation in favor of adipogenic differentiation from hMSCs. PMID: 21289500
    14. SSH1 plays a critical role in cytokinesis by dephosphorylating and reactivating cofilin in later stages of mitosis PMID: 12807904
    15. findings suggest that SSH1L is activated downstream of PI3K and plays a critical role in insulin-induced membrane protrusion by dephosphorylating and activating cofilin PMID: 14645219
    16. SSH1L is locally activated by translocation to and association with F-actin in lamellipodia in response to neuregulin-1beta and 14-3-3 proteins negatively regulate SSH1L activity by sequestering it in the cytoplasm. PMID: 15159416
    17. SSH1 mutations seen in patients with disseminated superficial actinic porokeratosis PMID: 15459975
    18. a multi-protein complex consisting of SSH-1L, LIMK1, actin, and the scaffolding protein, 14-3-3zeta, is involved, along with the kinase, PAK4, in the regulation of ADF/cofilin activity PMID: 15660133
    19. Ca2+-induced cofilin dephosphorylation is mediated by calcineurin-dependent activation of SSH1L PMID: 15671020
    20. identified sites that play unique roles in the control of subcellular localization and F-actin-mediated activation of SSH1L PMID: 16513117
    21. Expression of phosphatase-dead versions of SSH proteins, SSH1, SSH2 & SSH3 results in phosphorylation/inactivation of cofilin, changes in cytoskeleton organization, loss of cell polarity, and assembly of aberrant arrays of laminin-332 in keratinocytes. PMID: 17848544
    22. PDGF participates in actin dynamics by dual regulation of cofilin activity via LIMK and SSH1L in aortic smooth muscle cells PMID: 18096821
    23. the conserved SSH-N domain of slingshot-1 plays critical roles in P-cofilin recognition, F-actin-mediated activation, and subcellular localization of SSH1 PMID: 18809681
    24. Results suggest that the formation of ROS by NADPH oxidases engages a SSH-1L-cofilin pathway to regulate cytoskeletal organization and cell migration. PMID: 19339277
    25. These results implicate 14-3-3zeta/tau heterodimers as key regulators of SSH1 activity in keratinocytes and suggest they play a role in cytoskeleton remodeling during cell migration. PMID: 19371722
    26. Data identify PKD as a central regulator of the cofilin signaling network via direct phosphorylation and regulation of SSH1L. PMID: 19567672

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cleavage furrow. Midbody. Note=Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
  • 蛋白家族：
    Protein-tyrosine phosphatase family
  • 数据库链接：

    HGNC: 30579

    OMIM: 606778

    KEGG: hsa:54434

    STRING: 9606.ENSP00000315713

    UniGene: Hs.199763