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SSX2IP Antibody

  • 货号：
    CSB-PA897526LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, Rat liver tissue
      All lanes: SSX2IP antibody at 3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 72, 66, 69 kDa
      Observed band size: 72 kDa
    • IHC image of CSB-PA897526LA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA897526LA01HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA897526LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SSX2IP Polyclonal antibody
  • Uniprot No.：
    Q9Y2D8
  • 基因名：
  • 别名：
    ADIP antibody; ADIP_HUMAN antibody; Afadin and alpha actinin binding protein antibody; Afadin DIL domain interacting protein antibody; Afadin DIL domain-interacting protein antibody; Afadin- and alpha-actinin-binding protein antibody; FLJ10848 antibody; KIAA0923 antibody; MGC75026 antibody; OTTHUMP00000011574 antibody; SSX 2 interacting protein antibody; SSX2 interacting protein antibody; SSX2-interacting protein antibody; SSX2IP antibody; Synovial sarcoma X breakpoint 2 interacting protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human Afadin- and alpha-actinin-binding protein (485-614AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SSX2IP Antibody (CSB-PA897526LA01HU)，的标记方式是Non-conjugated。对于SSX2IP Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA897526LB01HU SSX2IP Antibody, HRP conjugated ELISA
    FITC CSB-PA897526LC01HU SSX2IP Antibody, FITC conjugated
    Biotin CSB-PA897526LD01HU SSX2IP Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling. Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Involved in ciliogenesis. It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia.
  • 基因功能参考文献：
    1. This study determined the crystallographic structure of human Afadin in complex with LGN. PMID: 26751642
    2. Studied afadin in complex with beta-catenin in glomeruli and in cultured mesangial cells. Found expression of afadin at mesangial intercellular junctions was greatly decreased in mesangial proliferative nephritis in rats & patients w/glomerulonephritis. PMID: 26568295
    3. Knockdown of Wdr8 or hMsd1/SSX2IP displayed very similar mitotic defects, in which spindle microtubules became shortened and misoriented. PMID: 26545777
    4. SSX2IP-mediated microtubule anchoring is essential for proper centriole assembly and duplication. On SSX2IP knockdown, the centriolar satellites become stuck at the microtubule minus end near the centrosome. PMID: 25833712
    5. results showed that SSX2IP promotes the development and metastasis of hepatocellular carcinoma and contributes to the drug resistance of hepatoma cells PMID: 23452395
    6. Nuclear protein SS2IP interacts with cancer-related protein SSX2 and the human homologue of a Ras-like GTPase interactor, RABIN3. PMID: 12007189
    7. data shows that SSX2IP is affected in two ways that relate to the cell cycling of acute myeloid leukaemia cells PMID: 18028484

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  • 亚细胞定位：
    Cell junction, adherens junction. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body.
  • 蛋白家族：
    ADIP family
  • 组织特异性：
    Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain
  • 数据库链接：

    HGNC: 16509

    OMIM: 608690

    KEGG: hsa:117178

    STRING: 9606.ENSP00000340279

    UniGene: Hs.22587