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TNRC6A Antibody

  • 货号：
    CSB-PA839859LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA839859LA01HU at dilution of 1:100
    • Immunofluorescence staining of Hela cells with CSB-PA839859LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TNRC6A Polyclonal antibody
  • Uniprot No.：
    Q8NDV7
  • 基因名：
    TNRC6A
  • 别名：
    CAG repeat protein 26 antibody; CAGH26 antibody; DKFZp666E117 antibody; EDIE antibody; EMSY interactor protein antibody; FLJ22043 antibody; Glycine tryptophan protein of 182 kDa antibody; Glycine-tryptophan protein of 182 kDa antibody; GW1 antibody; GW182 antibody; GW182 autoantigen antibody; Hypothetical protein DKFZp566M143 antibody; KIAA1460 antibody; MGC75384 antibody; OTTHUMP00000122485 antibody; OTTHUMP00000195164 antibody; OTTHUMP00000195165 antibody; Protein GW1 antibody; TNR6A_HUMAN antibody; TNRC 6A antibody; TNRC6 antibody; Tnrc6a antibody; Trinucleotide repeat containing 6A antibody; Trinucleotide repeat containing gene 6A protein antibody; Trinucleotide repeat-containing gene 6A protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Trinucleotide repeat-containing gene 6A protein (1-161AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，TNRC6A Antibody (CSB-PA839859LA01HU)，的标记方式是Non-conjugated。对于TNRC6A Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA839859LB01HU TNRC6A Antibody, HRP conjugated ELISA
    FITC CSB-PA839859LC01HU TNRC6A Antibody, FITC conjugated
    Biotin CSB-PA839859LD01HU TNRC6A Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As a scaffolding protein, associates with argonaute proteins bound to partially complementary mRNAs, and can simultaneously recruit CCR4-NOT and PAN deadenylase complexes.
  • 基因功能参考文献：
    1. that the interactions observed between TNRC6A and importin-alpha are conserved between mouse and human complexes. Our results highlight the ability of monopartite cNLS sequences to maximise contacts at the importin-alpha major binding site, as well as regions outside the main binding cavities PMID: 28837617
    2. The results suggest that overexpressed Dcp1a and GW182 can form different cytoplasmic aggregates and play distinct biological roles in the miRNA pathway. PMID: 28488892
    3. The results indicate that TNRC6A subcellular localization is substantially controlled by the interaction with Argonaute proteins. PMID: 26446993
    4. TRIM65 relieves miRNA-driven suppression of mRNA expression through ubiquitination and subsequent degradation of TNRC6. PMID: 24778252
    5. we have identified a new function of GW182 in protecting mature miRNA from being degraded by interacting with Argonaute (AGO) proteins PMID: 23090477
    6. HSP90 and GW182 are important factors in the pathomechanism of alcohol-induced augmentation of HCV replication. PMID: 22898980
    7. GW182 plays a critical role in miRNA-mediated gene silencing. (Review) PMID: 23224966
    8. GW182 proteins function as scaffold proteins for the assembly of the multiprotein complex that silences miRNA targets. (Review) PMID: 23224969
    9. 28.6% of patients containing autoantibodies to the trinucleotide repeat region of the TNRC6A were shown to have a single nucleotide polymorphism at c.344C>A in the CAG/CCA/G-rich region of TNRC6A. PMID: 23224974
    10. Sinse Argonaute and GW182 are the core proteins of the RNA-silencing complex (RISC), it seems appropriate to suggest that the RISC is the main target of human autoantibodies. (Review) PMID: 23224975
    11. Analysis of the results suggested that TNRC6A plays an important role in navigating Argonaute protein into the nucleus to lead miRNA-mediated gene silencing. PMID: 23150874
    12. Mammalian GW proteins have distinctive functions in the miRNA pathway, with GW220/TNGW1 being essential for aggregation and formation of GW/P bodies that sequester and stabilize translationally repressed target mRNA. PMID: 22891262
    13. Data demonstrate that in human and Drosophila melanogaster cells, the critical repressive features of both the N-terminal and C-terminal effector domains of GW182 proteins are Gly/Ser/Thr-Trp (G/S/TW) or Trp-Gly/Ser/Thr (WG/S/T) motifs. PMID: 21984184
    14. Data suggest that GW182 serves as both a platform that recruits deadenylases and as a deadenylase coactivator that facilitates the removal of the poly(A) tail by CCR4-NOT. PMID: 21984185
    15. Alanine substitution showed that GW/WG motifs in Delta12 (Delta12a, amino acids 896-1045) were important for the silencing activity of GW182. PMID: 21131274
    16. GW ribonucleoprotein complex is involved in the posttranscriptional regulation of gene expression PMID: 11950943
    17. gene knockdown of GW182 led to the disappearance of GW bodies demonstrating that GW182 is a critical component of GW bodies PMID: 15494374
    18. data support a model in which GW182 and/or the microenvironment of the cytoplasmic GWBs contribute to the RNA-induced silencing complex and to RNA silencing PMID: 16284622
    19. results support a functional link between GW182 and the ability of a microRNA to repress expression of a target mRNA PMID: 16284623
    20. A novel 210-kDa isoform of human GW182, provisionally named trinucleotide GW1 (TNGW1)was reported.TNGW1 was expressed independently of GW182 and was present in human testis and various human cancer cells. PMID: 19056672
    21. Our findings indicate that TNRC6A, is recruited to miRNA targets through an interaction between their N-terminal domain and an Argonaute protein PMID: 19383768

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  • 亚细胞定位：
    Cytoplasm, P-body.
  • 蛋白家族：
    GW182 family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 11969

    OMIM: 610739

    KEGG: hsa:27327

    STRING: 9606.ENSP00000379144

    UniGene: Hs.655057