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TOP2B Antibody

  • 货号：
    CSB-PA587958
  • 规格：
    ￥2024
  • 图片：
    • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Topoisomerase II β antibody.
    • Western blot analysis of extracts from Jurkat cells, using Topoisomerase II β antibody.
    • Western blot analysis of extracts from K562 cells (Lane 2), using TOP2B antiobdy. The lane on the left is treated with synthesized peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TOP2B Polyclonal antibody
  • Uniprot No.：
    Q02880
  • 基因名：
    TOP2B
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from Internal of Human topoisomerase II β.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand.
  • 基因功能参考文献：
    1. our data primarily indicate that topoIIbeta can be used to estimate neuronal differentiation in medulloblastoma PMID: 28554742
    2. The article discusses origin of topoisomerase II beta, its structure, activities reported in vitro and in vivo along with implications in cellular processes namely transcription, DNA repair, neuronal development, aging, HIV-infection and cancer. (Review) PMID: 28669856
    3. recent data point to a critical role of topoisomerase II beta (TOP2B), which is a primary target of anthracycline poisoning, in the pathophysiology of anthracycline-induced congestive heart failure . PMID: 28102848
    4. The decatenation checkpoint is regulated, not only by topo IIalpha, as previously reported, but also by topo IIbeta. The decatenation checkpoint is most efficient when both isoforms are present. Deletion of most of the C-terminus of topo IIbeta, while preserving the nuclear localization signal, and mutation of Y656 in topo IIbeta inhibits the decatenation checkpoint and sensitivity to topo II-targeted drugs. PMID: 28472494
    5. TOP2B is positioned to solve topological problems at diverse cis-regulatory elements and its occupancy is a highly ordered and prevalent feature of CTCF/cohesin binding sites that flank TADs. PMID: 27582050
    6. TopoIIbeta is involved in the cascade of coactivator complexes that are recruited to LTR for regulation of HIV-1 transcription. PMID: 26876283
    7. A direct interaction between Ku70/86 and BRG1 brings together SWI/SNF remodeling capabilities and TOP2beta activity to enhance the transcriptional response to hormone stimulation. PMID: 26055322
    8. Human cytomegalovirus IE1 exon 4 interacts with Topoisomerase IIbeta (TOPOIIbeta), whose activity is required for viral genome persistence and maintenance via binding to a cis-acting viral maintenance element. PMID: 25011107
    9. This study demonstrated that top IIbeta overexpression is necessary for RA-induced neuronal differentiation. PMID: 24801450
    10. Data suggest that DNA topoisomerase IIbeta (topoIIbeta) silencing can significantly alter the gene expression pattern of genes involved in variety of biological processes and signal transduction pathways. PMID: 25217229
    11. There is an association between chemosensitivity and Pgp, GST-pi and Topo II expression in gastric cancer. PMID: 24326092
    12. These results are consistent with the hypothesis that etoposide quinone contributes to etoposide-related leukemogenesis through an interaction with topoisomerase IIbeta. PMID: 24766193
    13. Downregulating topoisomerase IIbeta confers resistance specifically to mitoxantrone. PMID: 23696245
    14. Top2beta-DNA cleavage complexes arrest transcription elongation and induce a proteasomal degradation of Top2beta on DNA. PMID: 23938298
    15. results suggest that the low expression of TOPOIIbeta in patients with tongue carcinoma indicates that intrinsic drug resistance may exist in tongue carcinoma, and is associated with tumor differentiation and cisplatin resistance in tongue carcinoma. PMID: 21793937
    16. Overexpression of Lewis(y) antigen confers cell adhesion-mediated drug resistance to apoptosis in ovarian cancer cells by the upregulation of Topo-I and Topo-II beta. PMID: 21542140
    17. Tyrosine 656 in topoisomerase II beta is important for the catalytic activity of the enzyme. PMID: 21280220
    18. [review] Accumulating studies show the significant role of Top IIbeta in neuronal development through regulating expression of certain genes in cells committed to the neuronal fate after the final division. PMID: 20882068
    19. shows for the first time that topoisomerase II beta is a substrate for PKC zeta, and that PKC zeta may significantly influence topoisomerase II inhibitor-induced cytotoxicity by altering topoisomerase II beta activity through its kinase function PMID: 12105221
    20. analysis of binding sites for NF-Y and Sp1, which are critical for TOP2B transcription PMID: 12197834
    21. proteasomal degradation of TOP2beta induced by the TOP2-DNA covalent complex or the TOP2 circular clamp is due to transcriptional arrest but not DNA damage PMID: 12629207
    22. Topoisomerase II is a potential target for new antilesishmanial drug development. PMID: 14723357
    23. When both topo IIalpha and topo IIbeta were removed, the segregation of chromosomes was severely arrested, suggesting that topo IIbeta could partially substitute for topo IIalpha. PMID: 14996935
    24. TOP2A may have a role in response to doxorubicin-based chemotherapy PMID: 16502015
    25. reported that signal-dependent activation of gene transcription by nuclear receptors and other classes of transcription factors requires DNA topoisomerase IIbeta-dependent, transient, site-specific dsDNA break formation PMID: 16794079
    26. Reduced expression of topo IIbeta induces apoptosis in part by impairing the anti-oxidant capacity of the cell owing to downregulation of PRDX2. PMID: 16932348
    27. EGF may have a role in drug resistance in cultured tumor cells through the downregulation of topoII PMID: 16969495
    28. directed mutation of TOP2B demonstrated missense mutations selected for acridine resistance varied with acridine structure PMID: 17209120
    29. The C-terminal region of human topoisomerase II beta determines its isoform-specific functions in proliferating cells. PMID: 17526531
    30. TopoIIbeta binds a retinoic acid response element. Inhibition causes hyperacetylation of histone 3 at lysine 9 & transcription activation.Increased levels of & association with TopoIIbeta cause resistance to RA in acute promyelocytic leukemia cell lines. PMID: 18212063
    31. NRF-1 can also directly interact with poly(ADP-ribose) polymerase 1 (PARP-1) and co-purify the PARP-1.DNA-PK.Ku80.Ku70.topoisomerase IIbeta-containing protein complex. PMID: 19181665

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  • 相关疾病：
    A defect in TOP2B is associated with autism spectrum disorder (ASD).
  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus.
  • 蛋白家族：
    Type II topoisomerase family
  • 组织特异性：
    Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. Also found in breast, colon and lung carcinomas, Hodgkin's disease, large-cell non-Hodgkin's lymphoma, lymphocytic lymphomas and seminomas.
  • 数据库链接：

    HGNC: 11990

    OMIM: 126431

    KEGG: hsa:7155

    STRING: 9606.ENSP00000396704

    UniGene: Hs.475733