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TRIM13 Antibody

  • 货号：
    CSB-PA024450ESR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: TRIM13 antibody at 1.44µg/ml
      Lane 1: 293T whole cell lysate
      Lane 2: Hela whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 47, 20, 48 kDa
      Observed band size: 47 kDa
    • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA024450ESR2HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA024450ESR2HU at dilution of 1:100
  • 其他：

产品详情

  • 产品描述：

    The immunization of rabbits with the recombinant human TRIM13 protein (40-310AA) and final purification from rabbit antiserum by antigen affinity purification causes the creation of the anti-TRIM13 antibody. This TRIM13 antibody is a polyclonal antibody and exists as an unconjugated IgG. It only shows reactivity with human TRIM13 protein, a RING-type E3 ubiquitin ligase that ubiquitinates L-type channels and induces proteasomal degradation. The TRIM13 protein also enhances apoptosis through protein kinase B, modulates endoplasmic reticulum stress-caused autophagy, affects tumor cell growth, and participates in the regulation of melanoma differentiation-related antigen 5-mediated type I interferon generation. And this anti-TRIM13 antibody has been validated for use in ELISA, WB, and IHC applications.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TRIM13 Polyclonal antibody
  • Uniprot No.：
    O60858
  • 基因名：
    TRIM13
  • 别名：
    TRIM13; LEU5; RFP2; RNF77; E3 ubiquitin-protein ligase TRIM13; B-cell chronic lymphocytic leukemia tumor suppressor Leu5; Leukemia-associated protein 5; Putative tumor suppressor RFP2; RING finger protein 77; RING-type E3 ubiquitin transferase TRIM13; Ret finger protein 2; Tripartite motif-containing protein 13
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human E3 ubiquitin-protein ligase TRIM13 protein (40-310AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Plays also a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation. Participates as well in T-cell receptor-mediated NF-kappa-B activation. In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B.
  • 基因功能参考文献：
    1. Results from a study on gene expression variability markers in early-stage human embryos shows that TRIM13 is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
    2. TRIM13 mediated NF-kappaB repression is essential for negative regulation of clonogenic ability of the cells. PMID: 25152375
    3. Results show that HRD1 and RFP2 contributes are required for the disposal of V247M alpha-sarcoglycan mutant. PMID: 24565866
    4. This study first time demonstrated the role of TRIM13 as novel regulator of caspase-8 activation and cell death during endoplasmic reticulum stress. PMID: 24021263
    5. Shows important roles of TRIM13 in MM tumour survival and proliferation in multiple myeloma. PMID: 23647456
    6. Results describe the role of TRIM13 in induction of autophagy, which may play an important role in regulation of ER stress and tumor suppression. PMID: 22178386
    7. Data suggest that irradiation causes RFP2 overexpression, which enhances ionizing radiation-induced apoptosis by increasing p53 stability and decreasing AKT kinase activity through MDM2 and AKT degradation. PMID: 21333377
    8. Rfp2 contains a C-terminal transmembrane domain indispensable for its localization to the ER PMID: 17314412
    9. TRIM13, also known as RFP2, promoter area contains multiple quadruplex forming GGGGA-repeats that possesses unusually hight activity, anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. PMID: 16499869

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Note=Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum.
  • 蛋白家族：
    TRIM/RBCC family
  • 数据库链接：

    HGNC: 9976

    OMIM: 605661

    KEGG: hsa:10206

    STRING: 9606.ENSP00000298772

    UniGene: Hs.436922