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TRIM35 Antibody

  • 货号：
    CSB-PA892166LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA892166LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA892166LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA892166LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TRIM35 Polyclonal antibody
  • Uniprot No.：
    Q9UPQ4
  • 基因名：
    TRIM35
  • 别名：
    0710005M05Rik antibody; A430106H13Rik antibody; AW046487 antibody; Hemopoietic lineage switch protein 5 antibody; HLS5 antibody; KIAA1098 antibody; Macrophage derived apoptosis inducing RBCC protein antibody; MAIR antibody; MGC17233 antibody; mKIAA1098 antibody; NC8 antibody; Protein MAIR antibody; Protein Nc8 antibody; TRI35_HUMAN antibody; TRIM35 antibody; Tripartite motif containing 35 antibody; Tripartite motif containing protein 35 antibody; Tripartite motif-containing protein 35 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Tripartite motif-containing protein 35 protein (1-206AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，TRIM35 Antibody (CSB-PA892166LA01HU)，的标记方式是Non-conjugated。对于TRIM35 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA892166LB01HU TRIM35 Antibody, HRP conjugated ELISA
    FITC CSB-PA892166LC01HU TRIM35 Antibody, FITC conjugated
    Biotin CSB-PA892166LD01HU TRIM35 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that participates in multiple biological processes including cell death, glucose metabolism, and in particular, the innate immune response. Mediates 'Lys-63'-linked polyubiquitination of TRAF3 thereby promoting type I interferon production via DDX58/RIG-I signaling pathway. Can also catalyze 'Lys-48'-linked polyubiquitination and proteasomal degradation of viral proteins such as influenza virus PB2. Acts as a negative feedback regulator of TLR7- and TLR9-triggered signaling. Mechanistically, promotes the 'Lys-48'-linked ubiquitination of IRF7 and induces its degradation via a proteasome-dependent pathway. Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth.
  • 基因功能参考文献：
    1. s subsequently investigated whether miR-4417 and TRIM35 regulate HCC cell proliferation and apoptosis through PKM2 Y105 phosphorylation, and the results supported our speculation that miR-4417 targets TRIM35 and regulates the Y105 phosphorylation of PKM2 to promote hepatocarcinogenesis. PMID: 28394882
    2. Data suggest that pyruvate kinase isoform M2 (PKM2)/tripartite motif-containing 35 (TRIM35) expression could be a biomarker for the prognosis of hepatocellular carcinoma (HCC) and target for cancer therapy. PMID: 25576919
    3. TRIM35 regulates the Warburg effect and tumorigenicity through interaction with PKM2 in hepatocellular carcinoma. PMID: 25263439
    4. gene located on chromosome 8p21, a region implicated in leukemias and solid tumors; enforced expression inhibited cell growth, clonogenicity, and tumorigenicity; conceivable HLS5 is one of the tumor suppressor genes thought to reside at the 8p21 locus PMID: 14662771
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    TRIM/RBCC family
  • 数据库链接：

    HGNC: 16285

    OMIM: 617007

    KEGG: hsa:23087

    STRING: 9606.ENSP00000301924

    UniGene: Hs.104223