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TRIM8 Antibody

  • 货号：
    CSB-PA871629LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA871629LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA871629LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TRIM8 Polyclonal antibody
  • Uniprot No.：
    Q9BZR9
  • 基因名：
    TRIM8
  • 别名：
    AA408830 antibody; BB006755 antibody; GERP antibody; Glioblastoma-expressed RING finger protein antibody; Lioblastoma expressed ring finger protein antibody; Probable E3 ubiquitin-protein ligase TRIM8 antibody; RING finger protein 27 antibody; RNF27 antibody; RP11-47A8.4 antibody; TRIM8 antibody; TRIM8_HUMAN antibody; Tripartite motif containing 8 antibody; Tripartite motif protein TRIM8 antibody; Tripartite motif-containing protein 8 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Probable E3 ubiquitin-protein ligase TRIM8 protein (367-517AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，TRIM8 Antibody (CSB-PA871629LA01HU)，的标记方式是Non-conjugated。对于TRIM8 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA871629LB01HU TRIM8 Antibody, HRP conjugated ELISA
    FITC CSB-PA871629LC01HU TRIM8 Antibody, FITC conjugated
    Biotin CSB-PA871629LD01HU TRIM8 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that participates in multiple biological processes including cell survival, differentiation, apoptosis, and in particular, the innate immune response. Participates in the activation of interferon-gamma signaling by promoting proteasomal degradation of the repressor SOCS1. Plays a positive role in the TNFalpha and IL-1beta signaling pathways. Mechanistically, induces the 'Lys-63'-linked polyubiquitination of MAP3K7/TAK1 component leading to the activation of NF-kappa-B. Modulates also STAT3 activity through negative regulation of PIAS3, either by degradation of PIAS3 through the ubiquitin-proteasome pathway or exclusion of PIAS3 from the nucleus. Negatively regulates TLR3/4-mediated innate immune response by catalyzing 'Lys-6'- and 'Lys-33'-linked polyubiquitination of TICAM1 and thereby disrupting the TICAM1-TBK1 interaction.
  • 基因功能参考文献：
    1. TRIM8 activates STAT3 by suppressing the expression of PIAS3, an inhibitor of STAT3, most likely through E3-mediated ubiquitination and proteasomal degradation. PMID: 28100038
    2. we provided evidence that TRIM8 and its regulators miR-17-5p and miR-106b-5 participate to a feedback loop controlling cell proliferation through the reciprocal modulation of p53, miR-34a and N-MYC. PMID: 28327152
    3. A pathogenic effect of a heterozygous truncating mutation in the tripartite motif containing 8 (TRIM8) gene on the postnatal development of the human brain. PMID: 27346735
    4. Our study provides evidences that TRIM8 may participate in the carcinogenesis and progression of glioma and that the transcriptional repression of TRIM8 might have potential value for predicting poor prognosis in glioma patients. PMID: 26077989
    5. These findings provide the first mechanistic link between TRIM8 and the drug resistance of clear cell Renal Cell Carcinoma PMID: 25277184
    6. Nucleo-cytoplasmic trafficking of TRIM8, a novel oncogene, is involved in positive regulation of TNF induced NF-kappaB pathway. PMID: 23152791
    7. TRIM8 is a p53 direct target gene that induces p53 stabilization and promotes the degradation of MDM2 protein. PMID: 22262183
    8. Tripartite motif 8 (TRIM8) modulates TNFalpha- and IL-1beta-triggered NF-kappaB activation by targeting TAK1 for K63-linked polyubiquitination. PMID: 22084099
    9. TRIM8 modulates translocation of phosphorylated STAT3 into the nucleus through interaction with Hsp90beta and consequently regulates transcription of Nanog in embryonic stem cells. PMID: 21689689
    10. These findings indicate that TRIM8 enhances the STAT3-dependent signal pathway by inhibiting the function of PIAS3. PMID: 20516148
    11. interacts with SOCS-1 PMID: 12163497
    12. Gerp transcribes interferon-gamma in epithelial and lymphoid cells and is expressed almost ubiquitously in tissues. PMID: 15345195
    13. Among genes correlated to nodal metastatic progression, we verified in vitro that NM23-H3 reduced cell motility and TRIM8 were a growth suppressor. PMID: 17900511

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    TRIM/RBCC family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 15579

    OMIM: 606125

    KEGG: hsa:81603

    STRING: 9606.ENSP00000302120

    UniGene: Hs.336810