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UBE2K Antibody

  • 货号：
    CSB-PA09667A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA09667A0Rb at dilution of 1:20
    • Immunofluorescence staining of Hela cells with CSB-PA09667A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) UBE2K Polyclonal antibody
  • Uniprot No.：
    P61086
  • 基因名：
  • 别名：
    E2 25K antibody; E2(25K) antibody; HIP-2 antibody; Huntingtin interacting protein 2 antibody; Huntingtin-interacting protein 2 antibody; HYPG antibody; LIG antibody; UBC1 antibody; UBE2K antibody; UBE2K_HUMAN antibody; Ubiquitin carrier protein antibody; Ubiquitin conjugating enzyme E2 25 kDa antibody; ubiquitin conjugating enzyme E2K antibody; ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody; Ubiquitin protein ligase antibody; Ubiquitin-conjugating enzyme E2 K antibody; Ubiquitin-conjugating enzyme E2(25K) antibody; Ubiquitin-conjugating enzyme E2-25 kDa antibody; Ubiquitin-conjugating enzyme E2-25K antibody; Ubiquitin-protein ligase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Ubiquitin-conjugating enzyme E2 K protein (2-200AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，UBE2K Antibody (CSB-PA09667A0Rb)，的标记方式是Non-conjugated。对于UBE2K Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA09667B0Rb UBE2K Antibody, HRP conjugated ELISA
    FITC CSB-PA09667C0Rb UBE2K Antibody, FITC conjugated
    Biotin CSB-PA09667D0Rb UBE2K Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:10-1:100
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • 基因功能参考文献：
    1. The crystal structure of a Ube2K~ubiquitin conjugate has been described, and it was shown that even though it is monomeric, Ube2K can synthesize Lys48-linked ubiquitin chains. PMID: 26592444
    2. Hip2, a ubiquitin-conjugating enzyme, can overcome radiation-induced G2/M cell cycle arrest and trigger the entry into mitosis. PMID: 23933584
    3. An extract of bark from the tropical rainforest plant Byrsonima crassifolia was screened for inhibition of diubiquitin formation by the human ubiquitin-conjugating enzyme E2-25K. PMID: 22164771
    4. one of the roles of the C-terminal UBA domain, in the context of E2-25K, is to increase processivity in Lys48-linked polyubiquitin chain synthesis, possibly through increased binding to the ubiquitinated substrate. PMID: 21281599
    5. Results suggest that the interaction between E2-25K and UBB(+1) is critical for the synthesis and accumulation of UBB(+1)-anchored polyubiquitin, which results in proteasomal inhibition and neuronal cell death. PMID: 20826778
    6. This study suggests that Hip2 might be involved in the regulation of Smac-mediated apoptosis. PMID: 20537984
    7. Results suggest that in permeabilized, US11-expressing cells polyubiquitination of the MHC class I heavy chain substrate can be catalyzed by E2-25K. PMID: 16868077
    8. E2-25K is involved in aggregate formation of expanded polyglutamine proteins and polyglutamine-induced cell death. PMID: 17092742

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 组织特异性：
    Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest leve
  • 数据库链接：

    HGNC: 4914

    OMIM: 602846

    KEGG: hsa:3093

    STRING: 9606.ENSP00000261427

    UniGene: Hs.50308