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USP28 Antibody

  • 货号：
    CSB-PA025718GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q96RU2
  • 基因名：
    USP28
  • 别名：
    Deubiquitinating enzyme 28 antibody; KIAA1515 antibody; Ubiquitin carboxyl terminal hydrolase 28 antibody; Ubiquitin carboxyl-terminal hydrolase 28 antibody; ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody; Ubiquitin specific peptidase 28 antibody; Ubiquitin specific processing protease 28 antibody; Ubiquitin specific protease 28 antibody; Ubiquitin thioesterase 28 antibody; Ubiquitin thiolesterase 28 antibody; Ubiquitin-specific-processing protease 28 antibody; UBP28_HUMAN antibody; USP 28 antibody; USP28 antibody; USP28 protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human USP28
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.
  • 基因功能参考文献：
    1. The s identified 53BP1 and USP28 as essential components acting upstream of p53, evoking p21-dependent cell cycle arrest in response not only to centrosome loss, but also to other distinct defects causing prolonged mitosis. PMID: 27371829
    2. USP28 controls activation of both the TP53 branch and the GATA4/NFkB branch that controls the senescence-associated secretory phenotype (SASP) PMID: 29089421
    3. 53BP1-USP28 cooperation is essential for normal p53-promoter element interactions and gene transactivation-associated events, yet dispensable for 53BP1-dependent DNA double-strand repair regulation. PMID: 27546791
    4. USP28-53BP1-p53-p21 signaling pathway is also required to arrest cell growth after a prolonged prometaphase. PMID: 27432896
    5. analysis of centrinone resistance identified a 53BP1-USP28 module as critical for communicating mitotic challenges to the p53 circuit and TRIM37 as an enforcer of the singularity of centrosome assembly. PMID: 27432897
    6. These results showed that USP28 is overexpressed in human glioblastomas and it contributes to glioma tumorigenicity. PMID: 26209720
    7. Data indicte that deubiquitinating enzyme USP28 was targeted by microRNA miR-4295. PMID: 25656529
    8. findings provide a first insight into understanding how the enzymatic activity of Usp28 is regulated by its non-catalytic UBR and endogenous ligands. PMID: 26268556
    9. identified Usp28 as a c-MYC target gene highly expressed in colorectal cancers, which indicates that USP28 and c-MYC form a positive feedback loop that maintains high c-MYC protein levels in tumors PMID: 24960159
    10. USP28 has a chain preference activity for Lys(11), Lys(48), and Lys(63) diubiquitin linkages PMID: 25359778
    11. Usp28 expression was indentified as a independent predictors of survival (P = 0.001) and potentially valuable in prognostic evaluation of bladder cancer PMID: 24347490
    12. Study reveals a critical mechanism underlying the epigenetic regulation by USP28. PMID: 24075993
    13. USP28 is not a critical factor in double-strand break metabolism and is unlikely to be an attractive target for therapeutic intervention aimed at chemotherapy sensitization. PMID: 24687851
    14. USP28 gene expression is down regulated by oxidative stress through the mediation of reactive oxygen species PMID: 23832602
    15. molecular cloning of USP28 & characterization of alternatively spliced products and tissue-specific isoforms PMID: 11597335
    16. Using a human cell line that faithfully recapitulated the Chk2-p53-PUMA pathway, we show that USP28 is required to stabilize Chk2 and 53BP1 in response to DNA damage. PMID: 16901786
    17. High expression levels of USP28 are found in colon and breast carcinomas, and stabilization of MYC by USP28 is essential for tumour-cell proliferation. PMID: 17558397

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  • 亚细胞定位：
    Nucleus, nucleoplasm.
  • 蛋白家族：
    Peptidase C19 family, USP28 subfamily
  • 数据库链接：

    HGNC: 12625

    OMIM: 610748

    KEGG: hsa:57646

    STRING: 9606.ENSP00000003302

    UniGene: Hs.503891