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USP48 Antibody

  • 货号：
    CSB-PA774808LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
      All lanes: USP48 antibody at 3.5μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 120, 113, 82, 46, 60, 18, 57, 121 kDa
      Observed band size: 120 kDa
    • IHC image of CSB-PA774808LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA774808LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA774808LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) USP48 Polyclonal antibody
  • Uniprot No.：
    Q86UV5
  • 基因名：
    USP48
  • 别名：
    AAH67261 antibody; Deubiquitinating enzyme 48 antibody; DKFZp762M1713 antibody; EC 3.1.2.15 antibody; Kiaa4202 antibody; MGC132556 antibody; MGC14879 antibody; RAP1GA1 antibody; Synaptic ubiquitin specific protease antibody; synUSP antibody; Ubiquitin carboxyl terminal hydrolase 48 antibody; Ubiquitin carboxyl-terminal hydrolase 48 antibody; Ubiquitin specific peptidase 48 antibody; Ubiquitin specific processing protease 48 antibody; Ubiquitin specific protease 31 antibody; Ubiquitin specific protease 48 antibody; Ubiquitin thioesterase 48 antibody; Ubiquitin thiolesterase 48 antibody; Ubiquitin-specific-processing protease 48 antibody; UBP48_HUMAN antibody; USP31 antibody; USP48 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Ubiquitin carboxyl-terminal hydrolase 48 protein (358-508AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，USP48 Antibody (CSB-PA774808LA01HU)，的标记方式是Non-conjugated。对于USP48 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA774808LB01HU USP48 Antibody, HRP conjugated ELISA
    FITC CSB-PA774808LC01HU USP48 Antibody, FITC conjugated
    Biotin CSB-PA774808LD01HU USP48 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites.
  • 基因功能参考文献：
    1. USP48 inactivation reduces chromosomal instability of Fanconi anemia (FA)-defective cells. Our results highlight a role for USP48 in controlling DNA repair and suggest it as a potential target that could be therapeutically exploited for FA. PMID: 29891926
    2. In contrast to other deubiquitinating enzymes (DUBs) that have been previously implicated in the regulation of Mdm2 protein stability, USP48 did not induce Mdm2 stabilization by significantly reducing Mdm2 ubiquitination levels. PMID: 28233861
    3. down-regulation of USP48 increases E-cadherin expression and epithelial barrier integrity through reducing TRAF2 stability PMID: 28874458
    4. study reveals that the USP48-Gli1 regulatory axis is critical for glioma cell proliferation and glioblastoma tumorigenesis. PMID: 28623188
    5. Data suggest that ubiquitin specific protease-48 (USP48) promotes genome stability by antagonizing BRCA1 E3 ligase function. PMID: 29335415
    6. this study demonstrates that USP48 controls the Ubiquitin/proteasome-system-dependent turnover of activated NF-kappaB/RelA in the nucleus. PMID: 25486460
    7. Northern blot analysis revealed a single USP31 transcript of approximately 4 kb, which was primarily expressed in the testis and lung. PMID: 15354349
    8. The Overexpression of USP31 in HEK 293T cells inhibited TNFalpha, CD40, LMP1, TRAF2, TRAF6 and IKKbeta-mediated NF-kappaB activation, but did not inhibit Smad-mediated transcription activation. PMID: 16214042
    9. data demonstrate a crucial role for the dynamic interaction between D3R and USP48 in the regulation of NHE3 expression and function PMID: 24308971

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Peptidase C19 family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 18533

    OMIM: 617445

    KEGG: hsa:84196

    STRING: 9606.ENSP00000309262

    UniGene: Hs.467524