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  4. USP8 Antibody

USP8 Antibody

  • 货号：
    CSB-PA025752ESR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: USP8 antibody at 14μg/ml + 293T whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 128, 116 kDa
      Observed band size: 128 kDa
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA025752ESR2HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) USP8 Polyclonal antibody
  • Uniprot No.：
    P40818
  • 基因名：
  • 别名：
    Deubiquitinating enzyme 8 antibody; FLJ34456 antibody; hUBPy antibody; HumORF8 antibody; KIAA0055 antibody; MGC129718 antibody; SPG59 antibody; Ubiquitin carboxyl-terminal hydrolase 8 antibody; Ubiquitin isopeptidase Y antibody; ubiquitin specific peptidase 8 antibody; Ubiquitin thioesterase 8 antibody; Ubiquitin-specific-processing protease 8 antibody; UBP8_HUMAN antibody; UBPY antibody; Usp8 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Ubiquitin carboxyl-terminal hydrolase 8 protein (1-270AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:200-1:1000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation.
  • 基因功能参考文献：
    1. High expression of USP8 was correlated with advanced tumor stage and high recurrence risk. Moreover, USP8 was identified as a novel independent prognostic factor for CSCC patients. PMID: 30010158
    2. In summary, we show that, contrary to the pituitary ACTH-secreting tumors, the USP8 hotspot sequence on exon 14 is not mutated in tumors causing ectopic ACTH secretion syndrome (EAS). Although USP8 is expressed in all EAS tumors examined, our in vitro data indicate that it is not involved in the regulation of ectopic proopiomelanocortin transcription. PMID: 28710116
    3. USP8 deubiquitinates Sec31A and inhibits the formation of large COPII carriers, thereby suppressing collagen IV secretion. PMID: 29604273
    4. review: role of USP8 driver mutations in Cushing's Disease PMID: 28850717
    5. Data (including data from studies using knockout and transgenic mice/cells) suggest that CLEC16A, NRDP1, and USP8 form tripartite complex; CLEC16A-NRDP1-USP8 complex appears to rely on ubiquitin signals to promote mitophagy and maintain mitochondrial function necessary for beta-cell function. (CLEC16A = C-type lectin domain family 16 member A; NRDP1 = ubiquitin-protein ligase NRDP1; USP8 = ubiquitin specific peptidase 8) PMID: 29180353
    6. the EGFR-USP8-trichoplein-Aurora A axis is a critical signaling cascade that restricts ciliogenesis in dividing cells, and functions to facilitate cell proliferation PMID: 29472535
    7. in human cells, Usp8 knockdown increased the lysosomal degradation of alpha-synuclein. PMID: 27444016
    8. Overexpression of USP8 in lung adenocarcinoma is an early event during the course of tumor progression, and is related to EGFR expression. PMID: 28544031
    9. Somatic mutations in USP8 are common in Nelson's tumors, and are associated with less favorable outcome after surgery. PMID: 28982703
    10. Data indicate that USP8 functions as a novel deubiquitylase of FLIPL and inhibits extrinsic apoptosis by stabilizing FLIPL. PMID: 27321185
    11. Somatic USP8 gene mutations are a common cause of pediatric Cushing disease due to pituitary adenoma. PMID: 28505279
    12. findings demonstrate that USP8 plays a key role in the trafficking and degradation of BACE1 by deubiquitinating lysine 501. PMID: 27302062
    13. USP8 regulates VEGFR2 trafficking, signaling and proteolysis. PMID: 26459808
    14. Ubpy loss of function results in the accumulation of autophagosomes due to a blockade of the autophagy flux. PMID: 26571504
    15. Results show that in addition to the scaffolding function in complex formation, BRUCE has an E3 ligase function to promote BRIT1 deubiquitination by USP8 leading to accumulation of BRIT1 at DNA double-strand break. PMID: 26683461
    16. No somatic mutations were observed in USP8 in a cohort of GH-secreting pituitary adenomas. PMID: 26701869
    17. Studies indicate that ubiquitin specific protease 8 protein (USP8) mutations contribute to adrenocorticotropic hormone (ACTH) overproduction in Cushing's disease (CD). PMID: 26285834
    18. The presence of USP8 mutations may predict favorable responses to the somatostatin analog pasireotide in corticotroph adenomas of Cushing's disease. PMID: 26578638
    19. The identification of USP8 mutations as contributors to the pathogenesis of ACTH-secreting pituitary adenomas represents an exciting advance in our understanding of Cushing's disease and its potential treatment into the of precision medicine. PMID: 25930709
    20. these findings implicate a novel role for K6-linked Ub conjugates and USP8-mediated deubiquitination in the regulation of PARK2 in mitochondrial quality control PMID: 25700639
    21. In surgically resected primary USP8-mutated tumor cells, USP8 knockdown or blocking EGFR effectively attenuates ACTH secretion. Inhibition of USP8 or EGFR is promising for treating USP8-mutated corticotrophin adenoma. PMID: 25675982
    22. USP8 is frequently mutated in adenomas causing Cushing's disease, especially in those from female adult patients diagnosed at a younger age. PMID: 25942478
    23. Haplotype CAAG may be a down-regulating factor for the risk of non-obstructive azoospermia. PMID: 25863102
    24. BRUCE acts as a scaffold, bridging the ubiquitin-specific peptidase 8 (USP8) and BRIT1 in a complex to coordinate USP8-catalyzed deubiquitination of BRIT1. PMID: 25733871
    25. USP8 is required for ci-M6PR endosomal localisation. PMID: 24894536
    26. Somatic USP8 mutations in corticotroph adenomas clustered in the 14-3-3 protein binding motif and enhanced catalysis and proteolytic cleavage, which increased EGF receptor deubiqutination and signaling. USP8 mutants enhanced POMC promoter activity. PMID: 25485838
    27. USP8, and the endosomal sorting protein, VPS28, are the negative regulators of NOD2-induced IL-8 secretion. PMID: 25170077
    28. This work uncovers a novel role for USP8-mediated deubiquitination of K6-linked ubiquitin conjugates from parkin in mitochondrial quality control. PMID: 25216678
    29. UBE2E3 and UBPY participate in the regulation of TDP-43 ubiquitination, solubility, and neurodegeneration PMID: 24825905
    30. USP8 maintains a basal expression of HIF1alpha and HIF transcriptional output in normoxia. PMID: 24378640
    31. USP8 is involved in deubiquitination of LRIG1, influencing the efficiency of Met degradation. PMID: 24828152
    32. Balanced reciprocal cross-regulation of RNF41 and USP8 determines whether receptors are sorted for lysosomal degradation or recycling, this way regulating basal cytokine receptor levels. PMID: 23750007
    33. No relationship was found in serum XIAP and USP8 levels with clinical parameters, response to chemotherapy, and survival in patients with advanced stages of non-small cell lung cancer. PMID: 24344018
    34. Knockdown of ubiquitin-specific peptidase 8 (USP8) selectively kills gefitinib-resistant non-small lung cancer cells. PMID: 23748694
    35. The ESCRT accessory protein HD-PTP/PTPN23 associates with epidermal growth factor receptor (EGFR) and combines with the deubiquitinating enzyme UBPY/USP8 to transfer EGFR from ESCRT-0 to ESCRT-III and drive EGFR sorting to intralumenal vesicles. PMID: 23477725
    36. Usp8 is a target for SRC-mediated tyrosine phosphorylation in the N-terminal 504 amino acids upon EGF stimulation of EGFR. PMID: 23333852
    37. Ubiquitin-specific peptidase 8 (USP8) regulates endosomal trafficking of the epithelial Na+ channel PMID: 23297398
    38. a UBPY-derived peptide can outcompete ubiquitin for STAM2 SH3 domain binding PMID: 22841719
    39. USP8 is a positive regulator in Hh signaling by down-regulating Smo ubiquitination and thereby mediating Smo intracellular trafficking. PMID: 22253573
    40. Studies indicate that USP8/Ubpy and AMSH interact with ESCRT components to modulate the ubiquitination status of receptors and relevant sorting proteins. PMID: 21448666
    41. UBPY regulates the level of protein ubiquitination on endosomes, which is required for maintaining the morphology of the organelle PMID: 16771824
    42. The USP8 recognition domain of NRDP1 has a novel protein fold that interacts with a conserved peptide loop of the rhodanese domain. PMID: 17035239
    43. UBPY-mediated EGFR de-ubiquitination promotes EGFR degradation PMID: 17121848
    44. UBPY MIT domain and another ubiquitin isopeptidase, AMSH, reveals common interactions with CHMP1A and CHMP1B but a distinct selectivity of AMSH for CHMP3/VPS24, a core subunit of the ESCRT-III complex, and UBPY for CHMP7. PMID: 17711858
    45. Data suggest that AMSH and UBPY are essential for trafficking and down-regulation of PAR(2) but not for regulating PAR(2) dissociation from beta-arrestin2 or PAR(2)-mediated ERK2 activation. PMID: 19684015

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  • 相关疾病：
    Pituitary adenoma 4, ACTH-secreting (PITA4)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Endosome membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Peptidase C19 family
  • 数据库链接：

    HGNC: 12631

    OMIM: 219090

    KEGG: hsa:9101

    STRING: 9606.ENSP00000302239

    UniGene: Hs.443731