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WAPL Antibody

  • 货号：
    CSB-PA772025LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA772025LA01HU diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA772025LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate
      All lanes: WAPL antibody at 2.5µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 133, 138, 143 kDa
      Observed band size: 133 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) WAPL Polyclonal antibody
  • Uniprot No.：
    Q7Z5K2
  • 基因名：
    WAPL
  • 别名：
    FOE antibody; Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody; Friend of EBNA2 protein antibody; KIAA0261 antibody; OTTHUMP00000020004 antibody; WAPAL antibody; WAPL antibody; WAPL_HUMAN antibody; Wings apart like homolog antibody; Wings apart like homolog (Drosophila) antibody; Wings apart-like protein homolog antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Wings apart-like protein homolog protein (1037-1190AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，WAPL Antibody (CSB-PA772025LA01HU)，的标记方式是Non-conjugated。对于WAPL Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA772025LB01HU WAPL Antibody, HRP conjugated ELISA
    FITC CSB-PA772025LC01HU WAPL Antibody, FITC conjugated
    Biotin CSB-PA772025LD01HU WAPL Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
  • 基因功能参考文献：
    1. Study shows that chromatin loop size can be increased and that the duration with which cohesin embraces DNA determines the degree to which loops are enlarged. Cohesin's DNA release factor WAPL restricts this loop extension and also prevents looping between incorrectly oriented CTCF sites. PMID: 28475897
    2. Rs7083506 and rs11202058 polymorphisms of hWAPL and their haplotype T-A were associated with cervical cancer. PMID: 26722608
    3. WAPL-depleted cells undergo anaphase with segregation errors, resulting in micronuclei and DNA damage. Stable WAPL depletion arrests cells in a p53-dependent manner but causes p53-deficient cells to become highly aneuploid. PMID: 24055153
    4. Wapl-C interacts with other cohesin subunits and possibly unknown effectors to trigger cohesin release from chromatin PMID: 23776203
    5. Depletion of Wapl, a negative cohesin regulator, rescues sister chromatid homologous recombination defects of Mms21-deficient or Scc1 mutant-expressing cells PMID: 22751501
    6. FOE encodes a protein of 1227 amino acids with a functional bipartite nuclear localization signal, an arginine-rich motif, a putative nuclear export signal as well as with three highly acidic regions and a predicted coiled-coil domain. PMID: 15383329
    7. Wapl is a new regulator of sister chromatid resolution and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits PMID: 17112726
    8. Wapl is required to unlock cohesin from a particular state in which it is stably bound to chromatin. PMID: 17113138
    9. our study suggests that unscheduled overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. PMID: 17382297
    10. isolated a large number of additional alternatively spliced WAPL variants from various cervical epithelia. Each variant consists of a variable 5'-terminal region and the conserved remainder. PMID: 18662753

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  • 亚细胞定位：
    [Isoform 2]: Nucleus.; Nucleus. Chromosome. Cytoplasm. Note=Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
  • 蛋白家族：
    WAPL family
  • 组织特异性：
    Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
  • 数据库链接：

    HGNC: 23293

    OMIM: 610754

    KEGG: hsa:23063

    STRING: 9606.ENSP00000298767

    UniGene: Hs.203099