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WNT10B Antibody

  • 货号：
    CSB-PA190943
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA190943(WNT10B Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Hela cell, Primary antibody: CSB-PA190943(WNT10B Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
  • 其他：

产品详情

  • Uniprot No.：
    O00744
  • 基因名：
  • 别名：
    Protein Wnt-10b antibody; Protein Wnt-12 antibody; SHFM6 antibody; wingless type MMTV integration site family; member 10B antibody; WN10B_HUMAN antibody; WNT 10B protein antibody; WNT 12 antibody; Wnt10b antibody; wnt12 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthetic peptide of Human WNT10B
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:500-1:2000
    IHC 1:10-1:50
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
  • 基因功能参考文献：
    1. Treatment of secondary hyperparathyroidism with calcitriol improved the bone anabolism by inhibiting osteoclasts and promoting osteoblasts that might be achieved by increasing the Wnt 10b level. PMID: 30149605
    2. We report an Indian girl with split-hand/foot malformation (SHFM), sparse hair, and interrupted eyebrows, who carries a novel homozygous deletion c.695_697delACA in WNT10B. The variant is deduced to cause an in-frame deletion of Asn residue 232 (p.Asn232del). PMID: 29427788
    3. Wnt10b is involved in forskolin/hCG-mediated BeWo cell fusion via beta-catenin/GCMa/syncytin pathway, which may also involve activation of PKA PMID: 28370659
    4. These results present the first evidence for a recurrent rearrangement promoted by a mobile ht-WNT10B oncogene, as a relevant mechanism for Wnt involvement in human cancer. PMID: 27853307
    5. High expression of WNT10B is associated with epithelial-mesenchymal transition and invasion of pancreatic cancer. PMID: 28586066
    6. this study provides novel insight into the mechanism of action of parathyroid hormone on human periodontal ligament cells and establish their interplay with T cells via the Wnt10b pathway as a modulating factor for the anabolic properties of the hormone in periodontal regeneration PMID: 28071181
    7. findings provide the identification of autosomal-dominant WNT10B mutations in individuals with oligodontia, which increases the spectrum of congenital tooth agenesis and suggests attenuated Wnt signaling in endothelial differentiation of dental pulp stem cells. PMID: 27321946
    8. these findings clearly demonstrate that Wnt10b promotes epidermal keratinocyte transformation through induced Egf pathway PMID: 25995040
    9. Data show that Wnt protein Wnt10b is expressed in cardiomyocytes and localized in the intercalated discs of mouse and human hearts. PMID: 26338900
    10. WNT10B enhances proliferation through beta-catenin and RAC1 GTPase in human corneal endothelial cells. PMID: 26370090
    11. Sequence analysis of WNT10B gene revealed a novel 4-bp deletion mutation. PMID: 24211389
    12. No association between WNT10B polymorphisms and adiposity parameters was found. However, a role for WNT10B variants in determining human bone mineral density was found. PMID: 23325361
    13. Variations in WNT10B do not contribute to human monogenic obesity in our population. PMID: 23104151
    14. Hypoxia-inducible factor-2alpha-dependent hypoxic induction of Wnt10b expression in adipogenic cells. PMID: 23900840
    15. we identified WNT10B as a direct target of miR-148a in cancer-associated fibroblasts from endometrial cancers PMID: 22890324
    16. WNT10B/beta-catenin signalling induces HMGA2 and proliferation in metastatic breast cancer tumours devoid of ERalpha, PR and HER2 expression. PMID: 23307470
    17. Results suggest that Wnt10b likely plays an important role in the development of endometrial cancer (EC). The results also identify a role for Wnt10b in EC cells through promoting proliferation and inhibiting apoptosis. PMID: 23135473
    18. A novel sequence variant (c.986C>G, p.Thr329Arg) of WNT10B has been identified in familial split-hand/foot malformations in a large consanguineous Pakistani family. PMID: 21554266
    19. common variation in WNT10B was shown to be associated with BMI and weight in a case-control population of Belgian males. PMID: 22189080
    20. Wnt10b, but not Wnt3a, stimulates the NFkappaB and Notch pathways in U2OS osteosarcoma cells. PMID: 21321991
    21. This study is the first report of the association of common genetic polymorphism of WNT10B with human fat accumulation. PMID: 20579865
    22. Homozygous nonsense mutation in WNT10B is associated with sporadic split-hand/foot malformation with autosomal recessive inheritance. PMID: 20635353
    23. pyrethroid insecticides and estrogen can enhance the expression of the WNT10B proto-oncogene PMID: 12437293
    24. mutations represent the first naturally occurring missense variants of WNT10B PMID: 16477437
    25. suggest that fibroblast growth factor switches WNT10B from a negative to a positive cell growth regulator PMID: 17761539
    26. Metastatic osteosarcoma cell lines showed better chemotaxis response to Wnt10B than the non-metastatic osteosarcoma cell lines PMID: 18465804
    27. By homozygosity mapping a novel Split-Hand/Foot Malformation locus at 12q13.11-q13 with a maximum multipoint lod score of 5.47; by subsequent candidate gene approach a homozygous missense WNT10b mutation was identified. PMID: 18515319
    28. This analysis implicates the WNT10B locus as a genetic element in the regulation of bone mass and structural geometry. PMID: 19016593
    29. In Wnt10b-expressing mammary tumors, levels of p27(KIP1) were extremely low; conversely, Wnt10b-null mammary cells expressed high levels of this protein, suggesting Wnt-dependent regulation of p27(KIP1). PMID: 19056892
    30. S1P induces osteoblast precursor recruitment and promotes mature cell survival. Wnt10b and BMP6 also were significantly increased in mature osteoclasts, whereas sclerostin levels decreased during differentiation. PMID: 19075223
    31. We have not found evidence for a robust association of common WNT10B gene allelic variants with either BMD or fractures in postmenopausal women. PMID: 19458884

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  • 相关疾病：
    Split-hand/foot malformation 6 (SHFM6); Tooth agenesis, selective, 8 (STHAG8)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
  • 数据库链接：

    HGNC: 12775

    OMIM: 225300

    KEGG: hsa:7480

    STRING: 9606.ENSP00000301061

    UniGene: Hs.91985