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XKR8 Antibody

  • 货号：
    CSB-PA875681LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate
      All lanes: XKR8 antibody at 3.4µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 45 kDa
      Observed band size: 45 kDa
    • IHC image of CSB-PA875681LA01HU diluted at 1:1000 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA875681LA01HU diluted at 1:1000 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA875681LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The product CSB-PA875681LA01HU is a polyclonal antibody raised in the rabbit against a partial recombinant protein corresponding to 69-158 amino acids of human XKR8 protein. It exists as the unconjugated IgG isoform. It only reacts with human samples. This XKR8 antibody was purified by protein G affinity chromatography with a purity of up to 95%. And it has tested its specificity and reliability in ELISA, WB, IHC, and IF applications.

    Its target protein XKR8 appears to play a role in cellular adhesion, migration, invasion, and survival. Its interactions with key regulators of these processes suggest that it may have important implications for the development and progression of cancer and other diseases.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) XKR8 Polyclonal antibody
  • Uniprot No.：
    Q9H6D3
  • 基因名：
    XKR8
  • 别名：
    FLJ10307 antibody; hXkr8 antibody; RP11-460I13.3 antibody; X Kell blood group precursor-related family; member 8 antibody; XK-related protein 8 antibody; XKR8 antibody; XKR8_HUMAN antibody; XRG8 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human XK-related protein 8 protein (69-158AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，XKR8 Antibody (CSB-PA875681LA01HU)，的标记方式是Non-conjugated。对于XKR8 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA875681LB01HU XKR8 Antibody, HRP conjugated ELISA
    FITC CSB-PA875681LC01HU XKR8 Antibody, FITC conjugated
    Biotin CSB-PA875681LD01HU XKR8 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:1000-1:2000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Phospholipid scramblase that promotes phosphatidylserine exposure on apoptotic cell surface. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment. Required for the clearance of apoptotic cells, such as engulfment of apoptotic germ cells by Sertoli cells, clearance of senescent neutrophils or regulation of bipolar cell numbers in the retina. Has no effect on calcium-induced exposure of phosphatidylserine. Promotes myoblast differentiation and survival.; (Microbial infection) Incorporated into Ebola virus-like particles, where its phospholipid scramblase activity is required to promote phosphatidylserine exposure on the surface of viral particles. Externalization of phosphatidylserine on the surface of viral particles is required for uptake by host cells.
  • 基因功能参考文献：
    1. Xkr8-BSG/NPTN complex is required for phosphatidylserine scrambling in apoptotic cell membranes. PMID: 27503893
    2. The Xkr8 influences myogenesis by acting as a positive regulator of terminal differentiation and myoblast survival. PMID: 28881496
    3. facilitates transbilayer migration of lipids [review] PMID: 26936867
    4. findings show the Xk-family protein Xkr8 mediates phosphatidylserine exposure in response to apoptotic stimuli; Xk-family proteins have evolutionarily conserved roles in promoting phagocytosis of dying cells by altering the phospholipid distribution in the plasma membrane PMID: 23845944
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cytoplasm, perinuclear region.
  • 蛋白家族：
    XK family
  • 数据库链接：

    HGNC: 25508

    KEGG: hsa:55113

    STRING: 9606.ENSP00000362991

    UniGene: Hs.55024