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ZRANB3 Antibody

  • 货号：
    CSB-PA705058LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA705058LA01HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ZRANB3 Polyclonal antibody
  • Uniprot No.：
    Q5FWF4
  • 基因名：
    ZRANB3
  • 别名：
    4933425L19Rik antibody; AH2 antibody; Annealing helicase 2 antibody; DNA annealing helicase and endonuclease ZRANB3 antibody; MGC105033 antibody; MGC75012 antibody; OTTHUMP00000197449 antibody; Zinc finger Ran-binding domain-containing protein 3 antibody; Zinc finger; RAN- binding domain containing 3 antibody; ZRAB3_HUMAN antibody; Zranb3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human DNA annealing helicase and endonuclease ZRANB3 protein (370-624AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ZRANB3 Antibody (CSB-PA705058LA01HU)，的标记方式是Non-conjugated。对于ZRANB3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA705058LB01HU ZRANB3 Antibody, HRP conjugated ELISA
    FITC CSB-PA705058LC01HU ZRANB3 Antibody, FITC conjugated
    Biotin CSB-PA705058LD01HU ZRANB3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.
  • 基因功能参考文献：
    1. PCNA and ATP-dependency serve as a multi-layered regulatory mechanism that modulates ZRANB3 activity at replication forks. PMID: 28621305
    2. A large number of SNF2 family, DNA and ATP-dependent motor proteins are needed during transcription, DNA replication, and DNA repair to manipulate protein-DNA interactions and change DNA structure. SMARCAL1, ZRANB3, and HLTF are three related members of this family with specialized functions that maintain genome stability during DNA replication. [review] PMID: 28954549
    3. depletion of SMARCAL1, a SNF2-family DNA translocase that remodels stalled forks, restores replication fork stability and reduces the formation of replication stress-induced DNA breaks and chromosomal aberrations in BRCA1/2-deficient cells. In addition to SMARCAL1, other SNF2-family fork remodelers, including ZRANB3 and HLTF, cause nascent DNA degradation and genomic instability PMID: 29053959
    4. Damage-induced fork reversal in mammalian cells requires PCNA ubiquitination, UBC13, and K63-linked polyubiquitin chains, previously involved in error-free damage tolerance. Fork reversal in vivo also requires ZRANB3 translocase activity and its interaction with polyubiquitinated PCNA, pinpointing ZRANB3 as a key effector of error-free DNA damage tolerance. PMID: 28886337
    5. Here is described a substrate recognition domain within ZRANB3 that is needed for it to recognize forked DNA structures, hydrolyze ATP, catalyze fork remodeling, and act as a structure-specific endonuclease. PMID: 26884333
    6. ZRANB3 translocase, a SNF2 family member related to the SIOD disorder SMARCAL1 protein, is recruited by polyubiquitinated PCNA to promote fork restart following replication arrest. PMID: 22704558
    7. AH2 is recruited to stalled replication forks and that cells depleted of AH2 are hypersensitive to replication stresses. PMID: 22705370
    8. a role for ZRANB3 in the replication stress response and suggest new insights into how DNA repair is coordinated with DNA replication to maintain genome stability. PMID: 22759634
    9. structural and functional differences between AH2 and HARP suggest that different annealing helicases have distinct functions in the cell. PMID: 21078962

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 数据库链接：

    HGNC: 25249

    OMIM: 615655

    KEGG: hsa:84083

    STRING: 9606.ENSP00000264159

    UniGene: Hs.658422