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inhA Antibody

  • 货号：
    CSB-PA363781HA01MVZ
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Mycobacterium tuberculosis inhA Polyclonal antibody
  • Uniprot No.：
    P9WGR1
  • 基因名：
    inhA
  • 别名：
    inhA antibody; Rv1484 antibody; MTCY277.05 antibody; Enoyl-[acyl-carrier-protein] reductase [NADH] antibody; ENR antibody; Enoyl-ACP reductase antibody; EC 1.3.1.9 antibody; FAS-II enoyl-ACP reductase antibody; NADH-dependent 2-trans-enoyl-ACP reductase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Mycobacterium tuberculosis
  • 免疫原：
    Recombinant Mycobacterium tuberculosis Enoyl-[acyl-carrier-protein] reductase [NADH] protein (1-269AA)
  • 免疫原种属：
    Mycobacterium tuberculosis
  • 标记方式：
    Non-conjugated

    本页面中的产品，inhA Antibody (CSB-PA363781HA01MVZ)，的标记方式是Non-conjugated。对于inhA Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA363781HB01MVZ inhA Antibody, HRP conjugated ELISA
    FITC CSB-PA363781HC01MVZ inhA Antibody, FITC conjugated
    Biotin CSB-PA363781HD01MVZ inhA Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Enoyl-ACP reductase of the type II fatty acid syntase (FAS-II) system, which is involved in the biosynthesis of mycolic acids, a major component of mycobacterial cell walls. Catalyzes the NADH-dependent reduction of the double bond of 2-trans-enoyl-[acyl-carrier protein], an essential step in the fatty acid elongation cycle of the FAS-II pathway. Shows preference for long-chain fatty acyl thioester substrates (>C16), and can also use 2-trans-enoyl-CoAs as alternative substrates. The mycobacterial FAS-II system utilizes the products of the FAS-I system as primers to extend fatty acyl chain lengths up to C56, forming the meromycolate chain that serves as the precursor for final mycolic acids.; Is the primary target of the first-line antitubercular drug isoniazid (INH) and of the second-line drug ethionamide (ETH). Overexpressed inhA confers INH and ETH resistance to M.tuberculosis. The mechanism of isoniazid action against InhA is covalent attachment of the activated form of the drug to the nicotinamide ring of NAD and binding of the INH-NAD adduct to the active site of InhA. Similarly, the ETH-NAD adduct binds InhA.
  • 基因功能参考文献：
    1. Out of the 141 isolates studied, 3 (2.1%) were found carrying mutations in the katG gene that confer resistance to Isoniazid (INH). No mutations were detected in the inhA promoter region gene that confer weak INH resistance or in the rpoB gene that confer Rifampicin resistance. PMID: 29868151
    2. Molecular dynamics (MD) simulation show that EN and N1 remained in the binding pocket similar to the docked pose of EN-InhA and E1-InhA complexes and also suggested that InhA binds to its inhibitor in inhibitor-induced folding manner. Our study concludes that multiple receptor conformers based molecular docking can be an alternative to study the effect of receptor flexibility in ligand binding PMID: 28957755
    3. Virtually Designed Triclosan-Based Inhibitors of Enoyl-Acyl Carrier Protein Reductase of Mycobacterium tuberculosis and of Plasmodium falciparum. PMID: 27490275
    4. Structural analysis of InhA provided the crucial points to enhance the NADH binding affinity towards InhA mutants in the presence of direct InhA inhibitors to combat isoniazid drug resistance. PMID: 26658674
    5. InhA gene mutation is associated with isoniazid resistance in Mycobacterium tuberculosis. PMID: 25799046
    6. The specific zone mutations in inhA gene for rifampicin and isoniazid were investigated with molecular methods in isolated unique PMID: 26506671
    7. Rational Modulation of the Induced-Fit Conformational Change for Slow-Onset Inhibition in Mycobacterium tuberculosis InhA. PMID: 26147157
    8. The study reports empirical parameters to differentiate the 'open' and 'closed' conformation of substrate-binding loop by comprehensive ana-lysis of InhA crystal structures. PMID: 24895891
    9. The results reveal that the presence of a mutation in the inhA regulatory region together with a mutation in the inhA coding region can lead to the development of high-level isoniazid resistance and cross-resistance to ethionamide among the MDR-TB strains circulating in Lisbon. PMID: 23539241
    10. No mutation was observed in the inhA promotor region or in the INH sensitive control isolate (H37Rv). PMID: 22358357
    11. We chose to search for new inhibitors of the enoyl-acyl carrier protein reductase InhA, the target of the first-line TB drug isoniazid. Two compounds (CD39 and CD117) were bactericidal against drug-susceptible and drug-resistant M. tuberculosis. PMID: 21628538
    12. inhA promoter mutations are strongly associated with extensively drug-resistant-tuberculosis in South Africa PMID: 21333101
    13. Phosphorylation of InhA inhibits mycolic acid biosynthesis and growth of Mycobacterium tuberculosis. PMID: 21143326
    14. InhA phosphorylation is an important event in regulating cell growth. PMID: 20864541
    15. inhA(S94A) allele confers clinically relevant levels of resistance to isoniazid killing & inhibition of mycolic acid synthesis. decreased binding of INH-NAD inhibitor to InhA establishes InhA as primary target of isoniazid action in M. tuberculosis. PMID: 16906155
    16. catalysis of substrate reduction by InhA results, in part, from correct orientation of the cofactor in the ground state PMID: 17472376
    17. T (-8)C of inhA mutations was found in multidrug resistant Mycobacterium tuberculosis isolates. PMID: 17539290
    18. The structure of two InhA mutants (I21V and S94A), identified in INH-resistant clinical isolates, were solved. PMID: 17588773
    19. inhibited by Anthecotulide from Anthemis auriculata PMID: 18424102

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  • 蛋白家族：
    Short-chain dehydrogenases/reductases (SDR) family, FabI subfamily
  • 数据库链接：

    KEGG: mtu:Rv1484

    STRING: 83332.Rv1484