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lepA Antibody

  • 货号：
    CSB-PA350834LA01ENV
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Escherichia coli lepA Polyclonal antibody
  • Uniprot No.：
    P60785
  • 基因名：
    lepA
  • 别名：
    lepA antibody; b2569 antibody; JW2553Elongation factor 4 antibody; EF-4 antibody; EC 3.6.5.n1 antibody; Ribosomal back-translocase LepA antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli Elongation factor 4 protein (1-599AA)
  • 免疫原种属：
    Escherichia coli
  • 标记方式：
    Non-conjugated

    本页面中的产品，lepA Antibody (CSB-PA350834LA01ENV)，的标记方式是Non-conjugated。对于lepA Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA350834LB01ENV lepA Antibody, HRP conjugated ELISA
    FITC CSB-PA350834LC01ENV lepA Antibody, FITC conjugated
    Biotin CSB-PA350834LD01ENV lepA Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.
  • 基因功能参考文献：
    1. The last 44 C-terminal amino acids of EF4 form a sub-domain within the C-terminal domain that is important for GTP-dependent function on the ribosome. PMID: 25712150
    2. Thus, EF4 has two opposing functions-at low-to-moderate levels of stress, the protein is protective by allowing stress-paused translation to resume; at high-levels of stress, EF4 helps bacteria self-destruct. PMID: 25491353
    3. (review) EF4 triggers back-translocation of the elongating ribosome, causing the translation machinery to move one codon backwards along the mRNA. PMID: 23662805
    4. Elevated intracellular Mg(2+) concentrations or low temperature inhibit protein synthesis, probably because of formation of aberrant elongating ribosomal states. We suggest that EF4 binds to these stuck ribosomes and remobilizes them PMID: 21300907
    5. EF4-dependent back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex via a four-step kinetic scheme (i.e., POST-->I(1)-->I(2)-->I(3)-->PRE PMID: 20045415
    6. LepA protein, present in all bacteria and mitochondria, is a third elongation factor required for accurate and efficient protein synthesis. PMID: 17110332
    7. The crystal structure of LepA from Escherichia coli at 2.8-A resolution, was determined. PMID: 18362332

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  • 亚细胞定位：
    Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, LepA subfamily
  • 数据库链接：

    KEGG: ecj:JW2553

    STRING: 316385.ECDH10B_2737