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tolC Antibody

  • 货号：
    CSB-PA355904XA01ENV
  • 规格：
    ￥880
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes:tolC Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 24 kDa
      Observed band size: 24 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Escherichia coli (strain K12) tolC Antibody Polyclonal antibody
  • Uniprot No.：
    P02930
  • 基因名：
    tolC Antibody
  • 别名：
    Outer membrane protein TolC (Multidrug efflux pump subunit TolC) (Outer membrane factor TolC) tolC colE1-i mtcB mukA refI toc weeA b3035 JW5503
  • 宿主：
    Rabbit
  • 反应种属：
    Escherichia coli (strain K12)
  • 免疫原：
    Recombinant Escherichia coli (strain K12) tolC protein (97-268aa)
  • 免疫原种属：
    Escherichia coli (strain K12)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.
  • 基因功能参考文献：
    1. TolC, a potentially interesting pharmaceutical target can be addressed by small molecules, blocking the channel. Biophysical characterization of the binding processes will support future identification and optimisation of more potent TolC blockers in order to validate TolC as a pharmaceutical target. PMID: 28746830
    2. The s report the near-atomic resolution cryoEM structures of the Escherichia coli AcrAB-TolC multidrug efflux pump in resting and drug transport states, revealing a quaternary structural switch that allosterically couples and synchronizes initial ligand binding with channel opening. PMID: 28355133
    3. A number of multi-drug efflux genes, particularly the central component TolC, show higher expression in antibiotic resistant Escherichia coli. PMID: 27105118
    4. Data suggest that outer membrane channel TolC plays an important role in extrusion of isoprenol and other medium-chain alcohols (CmOHs). PMID: 26563610
    5. Thus, tolC mutants grown in low iron conditions accumulate periplasmic enterobactin, which impairs bacterial morphology, possibly by sequestering iron and inhibiting an iron-dependent reaction involved in cell division or peptidoglycan synthesis. PMID: 24330203
    6. Interactions between TolC and AcrAB are highly dynamic and only a small fraction of TolC is utilized to support efflux activities. PMID: 23331412
    7. The TolC enhancement of extreme-acid survival includes Gad induction, but TolC-dependent growth rates below pH 6.5 do not involve Gad. PMID: 21541325
    8. These results indirectly suggest that TolC and YiaT proteins may play some role(s) in multidrug resistance. PMID: 20639525
    9. Inactivation of TolC triggers metabolic shutdown in E. coli cells grown in minimal glucose medium. PMID: 20545840
    10. essential for enterobactin export across the outer membrane PMID: 16166532
    11. The mechanism of tetracycline resistance was studied using expression studies in E. coli mutants that were defective in the tet(A) and the tolC proteins. PMID: 16687460
    12. Dada show that a combination of sbmA and tolC mutations appears to have additive effect, resulting in almost complete suppression of the phenotypic expression of Tn10 tetracycline resistance. PMID: 18083810
    13. Results describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state. PMID: 18406332
    14. TolC-dependent efflux system is involved in the exclusion of porphyrins in E. coli. PMID: 18641137
    15. The s conclude that TolC plays an important role in L: -cysteine tolerance probably due to its export ability and that TolC overexpression is effective for L-cysteine production in E. coli. PMID: 18828007
    16. Data that TolC is involved in the efflux of certain cellular metabolites that accumulate during growth and trigger the upregulation of MarA, SoxS, and Rob, which in turn upregulate tolC and help rid the bacteria of these metabolites. PMID: 19502391

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  • 亚细胞定位：
    Cell outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Outer membrane factor (OMF) (TC 1.B.17) family
  • 数据库链接：

    KEGG: ecj:JW5503

    STRING: 316385.ECDH10B_3209