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ACAA2 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA978277A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, A549 whole cell lysate, Mouse thymus
      All lanes: ACAA2 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42 kDa
      Observed band size: 42 kDa
    • IHC image of CSB-RA978277A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cell with CSB-RA978277A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 570-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The ACAA2 recombinant monoclonal antibody is produced through genetic engineering by cloning and expressing the ACAA2 monoclonal antibody-encoding gene. To generate the ACAA2 monoclonal antibody, a synthesized peptide derived from human ACAA2 protein was used as an immunogen. Affinity chromatography is employed to purify the obtained ACAA2 recombinant monoclonal antibody, ensuring high purity. This ACAA2 recombinant monoclonal antibody specifically recognizes and binds to the ACAA2 protein and has been validated for use in human samples. Its quality and specificity were tested through multiple applications such as ELISA, WB, IHC, and IF.

    The ACAA2 protein mainly catalyzes the beta-oxidation of fatty acids in the mitochondria, which involves the degradation of fatty acids to generate energy for various cellular processes. ACAA2 specifically catalyzes the formation of acetyl-CoA from long-chain acyl-CoA substrates, which is essential for fatty acid oxidation and energy metabolism. Additionally, ACAA2 plays a critical role in the regulation of lipid metabolism, lipid droplet formation, and lipid signaling pathways. Mutations in ACAA2 have been associated with various metabolic disorders, including hypoglycemia, hyperammonemia, and Reye syndrome.

  • Uniprot No.：
    P42765
  • 基因名：
    ACAA2
  • 别名：
    3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Acetyl-CoA acyltransferase) (Beta-ketothiolase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1), ACAA2
  • 反应种属：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human ACAA2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    12F4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation. Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
  • 基因功能参考文献：
    1. ACAA2 is a functional BNIP3 binding partner and provide a possible linkage between fatty acid metabolism and apoptosis of cells. PMID: 18371312
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Thiolase family
  • 数据库链接：

    HGNC: 83

    OMIM: 604770

    KEGG: hsa:10449

    STRING: 9606.ENSP00000285093

    UniGene: Hs.200136