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  4. ARG2 Recombinant Monoclonal Antibody

ARG2 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA216908A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA216908A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of PC-3 cell with CSB-RA216908A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 559-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing PC3 cells stained with CSB-RA216908A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    Through genetic engineering, the ARG2 recombinant monoclonal antibody is synthesized by cloning and expressing the ARG2 monoclonal antibody-encoding gene. The immunogen utilized to create the ARG2 monoclonal antibody is a synthesized peptide from the human ARG2 protein. The purified MAS1L recombinant monoclonal antibody is obtained through an affinity chromatography process that guarantees its high purity. This ARG2 recombinant monoclonal antibody can specifically recognize and bind to the human ARG2 protein. Four applications, including ELISA, IHC, IF, and FC, have been performed to validate the quality and specificity of the ARG2 recombinant monoclonal antibody.

    ARG2 is an enzyme that plays a crucial role in the urea cycle, which is a series of biochemical reactions that take place in the liver to eliminate excess nitrogen generated by the breakdown of amino acids. It catalyzes the hydrolysis of L-arginine to ornithine and urea, which are then utilized to form citrulline. In addition, ARG2 has been implicated in various physiological and pathological processes, including immune regulation, tissue repair, cancer, and neurological disorders.

  • Uniprot No.：
    P78540
  • 基因名：
    ARG2
  • 别名：
    Arginase-2, mitochondrial (EC 3.5.3.1) (Arginase II) (Kidney-type arginase) (Non-hepatic arginase) (Type II arginase), ARG2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human ARG2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9A4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells. May suppress inflammation-related signaling in asthmatic airway epithelium. May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism. In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF-alpha production during gestation. Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction. Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling. Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity. Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal.
  • 基因功能参考文献：
    1. xpression profiles revealed miR-1299 downregulation concomitant with arginase-2 (ARG2) upregulation in hyperpigmented skin of melasma patients. PMID: 28627081
    2. ARG2 promotes tumorigenesis, and HCMV may contribute to GBM pathogenesis by upregulating ARG2. PMID: 27363017
    3. fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2; results reveal a previously unappreciated role of dendritic cells within the developing fetus and indicate that they mediate homeostatic immune-suppressive responses during gestation PMID: 28614294
    4. Schizophrenia patients had increased arginase II protein expression in the frontal cortex compared to controls. PMID: 27529679
    5. our data provide compelling evidence that human cytomegalovirus reduced the level of microRNA-613 which functions as an anti-onco-miRNA in glioblastoma, primarily by downregulating the expression of arginase-2 PMID: 28718378
    6. overexpression of AMPK induced arginase II protein expression and viable cells numbers in human Pulmonary artery smooth muscle cell . PMID: 28213467
    7. Data show that transfection with Arginase II-small interfering RNA prevented hypoxia-induced cell proliferation. PMID: 27475813
    8. circulating Arginase 2 concentrations increase in clinical erectile dysfunction (ED) and are associated with increased risk for ED PMID: 26537638
    9. arginase 2 impairs endothelial autophagy independently of the L-arginine ureahydrolase activity through activation of RPS6KB1 and inhibition of PRKAA, which is implicated in atherogenesis PMID: 25484082
    10. High arginase II expression correlates with poor survival for patients with neuroblastoma. Neuroblastoma suppresses T-cell proliferation via arginase II expression and activity. PMID: 26054597
    11. high fat diet enhanced arginase-II expression/activity and p38mapk activity, which was associated with eNOS-uncoupling as revealed by decreased nitric oxide PMID: 25034973
    12. Arg-II, p38, and S6K1 form a positive circuit which regulates endothelial senescence and cardiovascular aging. PMID: 25635535
    13. OxLDL triggers retrograde translocation of arginase2 in aortic endothelial cells via ROCK and mitochondrial processing peptidase. PMID: 24903103
    14. These results demonstrate that S6K1 and arginase-II form a positive circuit mediating the detrimental effects of chronic L-arginine supplementation on endothelial cells. PMID: 24860943
    15. We speculate that cytomegalovirus may contribute to endothelial dysfunction via ARG II induction and reduced eNOS production. PMID: 24442486
    16. study identified arginase II as a new target of miR-17-5p in human pulmonary artery smooth muscle cells (hPASMC), with miR-17-5p involved in the hypoxic induction of arginase II in hPASMC; also found evidence supporting a feedback loop between arginase II and miR-17-5p, such that arginase II regulates miR-17-5p expression in hPASMC PMID: 24879052
    17. Data indicate that arginase II (Arg II) expressions were higher in breast tumor tissues compared to the matched normal. PMID: 24223914
    18. HDAC2 is a critical regulator of Arg2 expression and thereby endothelial nitric oxide and endothelial function. PMID: 24833798
    19. Arg-II promotes mitochondrial dysfunction leading to VSMC senescence/apoptosis through complex positive crosstalk among S6K1-JNK, ERK, p66Shc, and p53, contributing to atherosclerotic vulnerability phenotype. PMID: 23832324
    20. Maternal hypercholesterolemia in pregnancy alters umbilical vein reactivity because of fetal endothelial dysfunction associated with arginase and eNOS signaling imbalance. PMID: 23950140
    21. Gene expression studies have identified altered expression of arginase 2 in suicide completers with a history of mood disorders. PMID: 23260169
    22. presence of ARG2-expressing CAFs is an indicator of poor prognosis, as well as hypoxia, in pancreatic ductal carcinoma PMID: 23424623
    23. Endothelial eNOS/arginase imbalance contributes to vascular dysfunction in IUGR umbilical and placental vessels. PMID: 23122700
    24. arginase-II (ARG2) was expressed by 60 percent of head and neck squamous cell carcinomas; the absence of ARG2 expression was significantly associated with prolonged overall survival PMID: 22815199
    25. The inhibition of arginase II protein was found to be mediated by exchange protein directly activated by cAMP. PMID: 22447968
    26. This study demomistrated that H3K4me3 modification plays an important role in up regulation of ARG2 in prefrontal cortex. PMID: 22008221
    27. Data suggest that exposure to particulate matter (i.e., air pollutants) upregulates arginase II (but not arginase I) activity and expression in bronchial epithelial cells, in part, via epidermal growth factor-dependent signaling. PMID: 22712848
    28. DDIT3, STT3A, ARG2 and FAM129A immunohistochemistry does not appear to be useful in the diagnosis of thyroid follicular neoplasias, as they do not reliably distinguish follicular thyroid carcinoma from follicular thyroid adenoma. PMID: 22157935
    29. has a vascular role in placental vessels counteracting the NOS-dependent relaxation PMID: 22391327
    30. Sequence variations in the NOS2A and ARG2 loci were globally associated with exhaled nitric oxide PMID: 21039601
    31. Delineate a clearer path from OxLDL through the endothelial cell LOX-1 receptor, RhoA, and ROCK, to the activation of arginase II, downregulation of NO, and vascular dysfunction in atherosclerosis. PMID: 21130456
    32. gene silencing changed promotes apoptosis and reduced expression of cell proliferation markers in thyroid cancer cells PMID: 20542107
    33. In human lung microvascular endothelial cells, hypoxia upregulates arginase activity as well as mRNA and protein levels of arginase II. Inhibition of arginase II by small interfering RNA or by the inhibitor BEC enhanced NO levels. PMID: 20861464
    34. The alteration of arginase activity between colostrum and mature milk may be a consequence of the transfer of arginase from the blood of the breast mother mammary glands into the colostrum and mature milk PMID: 20853600
    35. Arginase contributes significantly to L-proline supply for collagen synthesis in rat fibroblasts, in which arginase I is the predominant isoenzyme, but not in human fibroblasts, in which arginase II is the only isoenzyme expressed PMID: 20107769
    36. Association of ARG2 gene polymorphism is present in adult asthma, has lower reversibility specific with beta2 agonists. It is also associated with asthma severity, severe airway hyperresponsiveness, and less long-term response to inhaled corticosteroids. PMID: 20124949
    37. There was an overexpression of arginase II in anorexia nervosa patients. PMID: 20071203
    38. [Review] Arginase is constitutively expressed in endothelial cells, but expression of the specific isoforms differs among mammalian species. Although some arginase I has been detected in human endothelial cells, the predominant isoform is arginase II. PMID: 19508396
    39. Plays a physiological role in male and female sexual arousal PMID: 12859189
    40. Increased arginase II expression and activity in pulmonary arterial hypertension. PMID: 15364894
    41. ArgII gene is an early IRF-3-regulated gene, which participates in the interferon-independent antiviral response through polyamine production and induction of apoptosis. PMID: 15955070
    42. The Asn149-->Asp mutation is proposed to generate a conformational change responsible for the altered substrate specificity of arginase II. PMID: 16128822
    43. Increasing L-Arg for NO synthesis by either arginase inhibition or direct L-Arg supplementation restores the age-related deficit in reflex cutaneous vasodilatation. PMID: 16675494
    44. observed in both cytotrophoblasts and syncytiotrophoblasts PMID: 16720041
    45. Increased arginase II expression & activity suggest a potential pathogenic role for platelet arginase and altered arginine and polyamine metabolism in sickle cell disease. PMID: 17353439
    46. arginase II expression may play a role in prostate cancer progression PMID: 18202758
    47. Cocoa flavonols lower ARG2 activity in endothelial cells in vitro and erythrocytes in vov. PMID: 18348861
    48. ARG2 is expressed in lung cancer, but it does not induce tumor immune escape and does not affect disease progression, most probably due to the lack of concomitant NOS expression. PMID: 18528866
    49. siRNA silencing of arginase-II but did not inhibit the up-regulation of endothelial VCAM-1, ICAM-1 and E-selectin by TNFalpha PMID: 19284655

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  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Arginase family
  • 组织特异性：
    Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas. Expressed in activated T cells.
  • 数据库链接：

    HGNC: 664

    OMIM: 107830

    KEGG: hsa:384

    STRING: 9606.ENSP00000261783

    UniGene: Hs.226007