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CARS Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA158403A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate
      All lanes: CARS antibody at 1:500
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 86, 83, 95 kDa
      Observed band size: 95 kDa
  • 其他：

产品详情

  • 产品描述：

    To generate the CARS recombinant monoclonal antibody, CUSABIO first obtained the CARS monoclonal antibody. The immunogen used to produce the CARS monoclonal antibody is a synthesized peptide from the human CARS protein. The gene encoding the MAS1L monoclonal antibody is cloned and expressed, and the resulting product is purified using affinity chromatography to ensure high purity. The CARS recombinant monoclonal antibody has been extensively validated for its specificity and quality, and it can recognize and bind specifically to the human CARS protein. This antibody has been tested and proven to work well in ELISA and WB applications.

    CARS is an enzyme that belongs to the class of ligases and specifically aminoacyl-tRNA synthetases. Its main function is to catalyze the attachment of cysteine to its corresponding tRNA molecule during protein synthesis. CARS plays an essential role in maintaining the accuracy and fidelity of protein synthesis by ensuring the correct amino acid is incorporated into the growing polypeptide chain. In addition to its primary function in protein synthesis, CARS has also been implicated in other cellular processes such as the regulation of cell growth and proliferation, signal transduction, and stress response.

  • Uniprot No.：
    P49589
  • 基因名：
    CARS
  • 别名：
    Cysteine--tRNA ligase, cytoplasmic (EC 6.1.1.16) (Cysteinyl-tRNA synthetase) (CysRS), CARS
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CARS
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    8B11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).
  • 基因功能参考文献：
    1. Four single nucleotide polymorphisms (SNPs) of CARS were significantly associated with gastric cancer risk in both the discovery stage and the validation stage PMID: 28409418
    2. The risk (G and C) and non-risk (C and T) allele frequencies of the SNPs at the rs1888747 and rs739401 loci of FRMD3 and CARS, respectively, did not differ significantly between the diabetics with (case) and without (control) nephropathy (P > 0.05). PMID: 26909942
    3. Loss of CARS, the cysteinyl-tRNA synthetase, suppresses ferroptosis induced by erastin, which inhibits the cystine-glutamate antiporter known as system xc(-). PMID: 26184909
    4. CARS is a novel fusion partner of anaplastic lymphoma kinase in anaplastic large-cell lymphoma and inflammatory myofibroblastic tumor cases. PMID: 12112524
    5. Specificity of anticodon recognition by human CysRS is higher than that of its bacterial counterparts. PMID: 17303165

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  • 相关疾病：
    A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I aminoacyl-tRNA synthetase family
  • 数据库链接：

    HGNC: 1493

    OMIM: 123859

    KEGG: hsa:833

    STRING: 9606.ENSP00000369897

    UniGene: Hs.274873