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  4. CD58 Recombinant Monoclonal Antibody

CD58 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA166177A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate
      All lanes: CD58 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 29, 27, 26 kDa
      Observed band size: 55-70 kDa
  • 其他：

产品详情

  • 产品描述：

    The production of the CD58 recombinant monoclonal antibody involves genetic engineering procedures that entail the cloning and expression of the CD58 monoclonal antibody-encoding gene. The synthesized peptide, derived from the human CD58 protein, serves as the immunogen for generating the CD58 monoclonal antibody. To ensure high purity, the CD58 recombinant monoclonal antibody is purified through affinity chromatography. The specificity of the CD58 recombinant monoclonal antibody allows it to recognize and bind to the CD58 protein with high precision. Rigorous validation and testing, including ELISA and WB applications, have confirmed the quality and specificity of the CD58 recombinant monoclonal antibody for use in human samples.

    CD58, also known as LFA-3, is a glycoprotein that is expressed on the surface of many types of cells, including B cells, T cells, and NK cells. Its main function is to bind to CD2, which is another glycoprotein found on the surface of T cells and NK cells, and this interaction facilitates the activation of these cells. CD58 also plays a crucial role in the immune response by regulating adhesion and co-stimulation between cells. It helps in the formation of the immunological synapse, which is a specialized interface between T cells and antigen-presenting cells, that allows for effective communication between these cells. It is also involved in the pathogenesis of certain diseases such as multiple sclerosis and rheumatoid arthritis, as it plays a role in the activation and migration of immune cells to inflamed tissues.

  • Uniprot No.：
    P19256
  • 基因名：
  • 别名：
    Lymphocyte function-associated antigen 3 (Ag3) (Surface glycoprotein LFA-3) (CD antigen CD58), CD58, LFA3
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CD58
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    13G5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
  • 基因功能参考文献：
    1. indicate that including both CD58 and CD81 markers in addition to CD19, CD34, CD20, CD38, and CD10 are helpful in minimal residual disease detection by flow cytometry PMID: 29500862
    2. Mutations and copy number loss of CD58 or TP53 are identified to be an independent negative prognostic factors for diffuse large B cell lymphoma. PMID: 27825110
    3. A new neuromyelitis optica spectrum disorders susceptibility variant was identified, rs56302466, on the CD58 gene, in a Han Chinese population. PMID: 28601281
    4. data suggest that loss of CD58 is a potential immune escape mechanism of HL tumor cells, especially in clinically aggressive disease PMID: 27467287
    5. Cytometry analysis evidenced a specific expression profile on reticulocytes of SCA infants, with notably an increased expression of the adhesion molecules Lu/BCAM, ICAM-4 and LFA-3, both in percentage of positive cells and in surface density. PMID: 26137540
    6. Frequent inactivating mutations of CD58 in classical Hodgkin lymphoma cell lines, but their rare occurrence in primary Hodgkin and Reed/Sternberg cells. PMID: 26194173
    7. SNPs in CD58 are associated with increased risk of candidemia. PMID: 25197941
    8. This study demonstrated that the Polymorphism, Single Nucleotide of CD58 is associated with multiple sclerosis in man in Russia. PMID: 25903733
    9. Results indicate that CD58 is a novel cell-surface marker that functionally regulates self-renewal of Colorectal tumor-initiating cells. PMID: 24727892
    10. Linkage disequilibrium show evidence that mir-548ac rs1414273 variant is strongly associated with Multiple sclerosis (MS)-associated haplotype and confirms the single nucleotide polymorphisms within the first intron of CD58 been related too. [review] PMID: 25795118
    11. The positive correlation is established between content of polymorphic nuclear monocytes and level of expression of molecules of LFA-1, ICAM-1, LFA-3, and PECAM-1. PMID: 25884075
    12. Genetic variations in CD58 were associated with the susceptibility of neuromyelitis optica in a Korean population. PMID: 24655566
    13. In 21 percent of diffuse large B cell lymphoma cases, lesions involve the CD58 gene, which encodes a molecule involved in T and natural killer cell-mediated responses PMID: 22137796
    14. Two large cohorts of systemic sclerosis (SSc) patients of European Caucasian ancestry do not support the implication of ITGAM, ITGAX, and CD58 genes in the genetic susceptibility of SSc, although they were identified as autoimmune disease risk genes. PMID: 21362770
    15. Seven selected CD58 single-nucleotide polymorphisms were found to not affect aspirin-exacerbated respiratory disease susceptibility in a Korean population. PMID: 21726122
    16. Studies indicate that SNP in IL7RA, IL2RA, CD58 and CLEC16A genes has been consistently associated with MS. PMID: 20450971
    17. Data show that coculture with activated T cells upregulated expression of CD54 and CD58 and secretion of galectin-1 by MSCs. PMID: 20570633
    18. Studies indicate that five SNPs showed genome-wide significant association with MS: HLA-DRA, IL7R, IL2RA, CD58 and CLEC16A. PMID: 19834503
    19. Signal-dependent adhesion of resting NK cells initiated by expression of ICAM-1 is greatly enhanced by coexpression of LFA-3, even in the absence of cytokines. PMID: 12496412
    20. The complement inhibitor CD59 and the lymphocyte function-associated antigen-3 (LFA-3, CD58) genes possess functional binding sites for the p53 tumor suppressor protein. PMID: 12553064
    21. Transmembrane CD58 may trigger signaling independently of the GPI-linked isoform. PMID: 15093607
    22. Human cells transformed with Ad12 demonstrated reduced expression of cell surface LFA-3. PMID: 15963548
    23. The level of CD58 molecule (in both serum and PBMC form) of patients with hepatitis B is related to the degree of liver damage. PMID: 16830383
    24. Susceptibility gene for multiple sclerosis in Australians. PMID: 18650830
    25. We found variable, but persistently elevated levels of sLFA-3 throughout the various phases and types of the hemorrhagic fever with renal syndrome, which suggest that sLFA-3 levels have correlation with disease stages. PMID: 18820826
    26. Cross-linking of CD58 induces protein tyrosine phosphorylation of BLNK, Syk and PLCgamma, and activation of ERK and Akt/PKB. PMID: 19268704
    27. Study reports additional genetic and transcriptomic evidence for the role of CD58 (LFA-3) in multiple sclerosis (MS) susceptibility using a Swedish case-control material, with a result that closely mimics that of De Jager et al. PMID: 19497873
    28. Genetic variants at CD58, is associated with rheumatoid arthritis risk PMID: 19898481

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein.
  • 数据库链接：

    HGNC: 1688

    OMIM: 153420

    KEGG: hsa:965

    STRING: 9606.ENSP00000358501

    UniGene: Hs.34341