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  4. MAS1L Recombinant Monoclonal Antibody

MAS1L Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA971033A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue
      All lanes: MAS1L antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 43 kDa
      Observed band size: 36-50 kDa
    • Immunofluorescence staining of MCF-7 cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA971033A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions.
  • 其他：

产品详情

  • 产品描述：

    The MAS1L recombinant monoclonal antibody is produced through a process of genetic engineering that involves cloning and expression of the gene encoding for the MAS1L monoclonal antibody. The immunogen used to generate the MAS1L monoclonal antibody is the synthesized peptide derived from human MAS1L protein. The obtained MAS1L recombinant monoclonal antibody is purified using affinity chromatography to ensure high and purity. This MAS1L recombinant monoclonal antibody can specifically recognize and bind to the MAS1L protein. It has been validated for use in human and rat samples. Multiple applications including ELISA, WB, IF, and FC have been carried out to test the quality and specificity of the MAS1L recombinant monoclonal antibody.

    MAS1L is primarily expressed in the adrenal gland, but is also found in other tissues including the brain, kidney, and heart. It is involved in the regulation of blood pressure and cardiovascular homeostasis through the renin-angiotensin system (RAS). MAS1L is activated by angiotensin-(1-7), a peptide hormone that opposes the effects of angiotensin II, which promotes vasoconstriction and raises blood pressure. Activation of MAS1L leads to vasodilation and a decrease in blood pressure. MAS1L has been found to be expressed on immune cells such as macrophages, and its activation has been shown to inhibit the release of inflammatory cytokines.

  • Uniprot No.：
    P35410
  • 基因名：
  • 别名：
    Mas-related G-protein coupled receptor MRG (MAS-R) (MAS1-like), MAS1L, MRG
  • 反应种属：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human MAS1L
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    15B8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 基因功能参考文献：
    1. Mas, MrgD, and MRG mediated Ang IV-stimulated AA release that was highest for Mas. While Ang III activated Mas and MrgX2, Ang II stimulated AA release via Mas and MRG. PMID: 18636314
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Mas subfamily
  • 数据库链接：

    HGNC: 13961

    OMIM: 607235

    KEGG: hsa:116511

    STRING: 9606.ENSP00000366331

    UniGene: Hs.533297