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ACE Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA268157A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA268157A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA268157A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    The zinc dicarboxypeptidase ACE is a key component of the renin-angiotensin system. It regulates blood pressure by cleaving angiotensin I (Ang I), a powerful vasoconstrictor, into angiotensin II (Ang II). Many studies have shown that ACE is a potent pro-inflammatory regulator that modulates chemokines and adhesion molecules to aid in the recruitment of inflammatory cells into tissues. Evidence has shown that ACE facilitates the immune activation of myeloid cells.

    CUSABIO designed the vector clones for the expression of a recombinant ACE antibody in mammalian cells. The vector clones were obtained by inserting the ACE antibody heavy and light chains into the plasma vectors. The recombinant ACE antibody was purified from the culture medium through Affinity-chromatography. It can be used to detect ACE protein from Human in the ELISA, IHC.

  • Uniprot No.：
    P12821
  • 基因名：
  • 别名：
    Angiotensin-converting enzyme (ACE) (EC 3.2.1.-) (EC 3.4.15.1) (Dipeptidyl carboxypeptidase I) (Kininase II) (CD antigen CD143) [Cleaved into: Angiotensin-converting enzyme, soluble form], ACE, DCP DCP1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human ACE1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    2H8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • 基因功能参考文献：
    1. The study suggests that the ACE I/D polymorphism may contribute to mechanisms and intensity of DNA damage in hypertensive and normotensive individuals. PMID: 30480916
    2. study found a significant association between the ACE insertion/deletion polymorphism and Kawasaki disease risk - meta-analysis PMID: 29937869
    3. ACE gene I/D polymorphism was not associated with the risk of chronic obstructive pulmonary disease. PMID: 29716409
    4. aerobic exercise training differentially affects the ACE C- and N-domain activities, and the N-domain activity is dependent on ACE polymorphism. PMID: 29629833
    5. A significant difference was found between the patients with familial Mediterranean fever (FMF)-related amyloidosis and the control group as for genotype distribution of angiotensin converting enzyme (ACE) I/D variant. Based on these observations, the ACE I/D variant D/D genotypes implicate a possible risk in the FMF-related amyloidosis among Turkish population. PMID: 29891744
    6. Angiotensin-converting enzyme insertion/deletion gene polymorphism is associated with prostate cancer susceptibility. PMID: 29970692
    7. This study aimed to investigate the relationship between estimated training status (TS), blood pressure and angiotensin-converting enzyme (ACE) activity in elderly people classified as low or high risk to develop hypertension according to genetic profile. PMID: 29923443
    8. This study shows that ACE gene polymorphism, particularly the D allele, is associated with worse functional outcome of ischaemic stroke patients. PMID: 29199539
    9. ACE gene polymorphisms were not associated with the development of preeclampsia in South African Black women. PMID: 29523271
    10. summary of current knowledge about circulating ACE elevation in patients with granulomatous and non-granulomatous diseases. PMID: 29928904
    11. This study found that patients with the DD genotype reduced the exogenous EPO requirement as compared to the II genotype. Further, D allele frequency was higher in patients with diabetic nephropathy as compared to I allele which implies that D allele is an independent risk factor for progression to CKD. PMID: 28457029
    12. Prevalence has been found of ACTN3 R577X and of ACE insertion / deletion gene polymorphisms in national and amateur Turkish athletes. PMID: 29729690
    13. The ACE and AGT gene polymorphisms are not associated with the progress of diabetes developing into retinopathy in Chinese patients with type 2 diabetes. PMID: 29378484
    14. Review/Meta-analysis: ACE I/D polymorphism may be a genetic molecular marker to predict systemic lupus erythematosus but not lupus nephritis. PMID: 29205894
    15. Association of insertion/deletion (I/D) polymorphism at angiotensin-converting enzyme gene (ACE) with depression in Chinese adolescents experiencing the 2008 Wenchuan earthquake. PMID: 28982269
    16. The ACE (I/D) gene polymorphism do not seem to have a significant effect on the development of clinical properties or cardiovascular comordities of acromegalic patients. PMID: 28712073
    17. polymorphisms in the eNOS "A/A" (homozygous mutant) and ACE "I/D" genotypes might contribute to the increased risk of NSCLC in the South Indian population. PMID: 27328622
    18. The study attempts to understand the distribution and extent of association of ACE I/D gene polymorphism with cardiometabolic risk factors among Bhils from rural and urban settings. PMID: 29435690
    19. Data suggest that the insertion/deletion (I/D) ACE (angiotensin converting enzyme) gene polymorphism may be associated with MI occurrence among younger patients. PMID: 29268798
    20. The analysis of genotype coexistence revealed a higher incidence of the combination of the ACE II and the PAI-1 4G/4G genotypes in the control group (10.0 vs.5.9% in control group; p = 0.17). CONCLUSIONS: The obtained results suggest no apparent association between the ACE I/D, PAI-1 4G/5G polymorphisms and increased RM susceptibility in the analyzed Polish population. PMID: 27321098
    21. An involvement of the ACE polymorphism in element imbalances in hypertension PMID: 28303511
    22. Concomitant presence of non-functional variant in ACTN3 gene decreased this beneficiary effect of ACE mutation on SBPR3. PMID: 29269700
    23. There were significant differences in sarcopenic obesity according to ACE I/D genotype, Women who were ACE DD presented lower risk of sarcopenic obesity than those in the ACE II and ACE ID groups PMID: 29130707
    24. Patients with Alzheimer's disease who were homozygous for angiotensin-converting enzyme (ACE) Insertion allele presented with a more rapid AD deterioration than did those who had other ACE genotypes, particularly those patients without hypertension. PMID: 27862810
    25. The ACE D allele may be predictive in individuals who may be at risk of developing CAD. Further investigations of these polymorphisms and their possible synergisms with traditional risk factors for CAD could help to ascertain better predictability for CAD susceptibility. PMID: 27895197
    26. ACE DD genotype and overall frequency of D allele is significantly higher in patients with PPCM. Also, the presence of DD genotype is associated with worse systolic performance indices measured echocardiographically. PMID: 29455790
    27. There was an independent association of the angiotensin-converting enzyme polymorphism with central and ambulatory blood pressure in Chinese patients with Hypertension. PMID: 28834200
    28. expression not increased in the maternal vascular endothelium in subjects with preeclampsia compared with normal pregnant controls PMID: 28878298
    29. DD genotype and D allele of ACE gene I/D polymorphism might increase the risk of lymph node metastasis in colorectal cancer patients. PMID: 29032382
    30. The results suggest that ACE I/D polymorphism, high ACE activity, body mass index and oxidative damage may play key roles in the pathogenesis of preeclampsia in the Mexican population. PMID: 29153683
    31. SNPs in folate pathway genes MTHFR/ MTR/ACE and hyperhomocysteinemia have roles in risk of coronary artery disease. PMID: 28514598
    32. ACE I alleles are associated with dementia risk. PMID: 28657841
    33. Data indicate it is unlikely that ACE II genotype provides an advantage in endurance running. PMID: 29298672
    34. This study concludes that DD genotype is strongly associated with higher SBP in hypertensive patients. PMID: 29058472
    35. ACE binds to lysozyme and bilirubin, which regulate its conformation and shedding PMID: 27734897
    36. The presence of the ACE I-allele was associated with increased aerobic functional capacity after the aerobic interval training program. PMID: 29846435
    37. Study showed that dietary weight loss-induced changes in angiotensin-converting enzyme activity, free fatty acids and RBP4 independently contribute to weight regain prediction. PMID: 29122953
    38. Among the four haplotypes composed by ACE gene A2350G and I/D, haplotype G-D reached the statistical significance in two groups, and exhibited to be a risk factor for the development of EH, whose P < 0.001 and OR 95%CI = 1.639(1.435-1.872), while the other haplotypes were the protective factors and decreased the susceptibility to EH(P < 0.05). PMID: 29172745
    39. The serum ACE level could be a novel noninvasive, easy, accurate, and inexpensive marker of significant fibrosis stage in patients with chronic hepatitis B. PMID: 29085215
    40. ACE SNPs were not associated with bone density among lead workers. PMID: 29028685
    41. Study showed the association between rs4343 and susceptibility to migraine in a case-control population. PMID: 28626926
    42. Studied interleukin-1 receptor antagonist (IL-1Ra) and angiotensin-converting enzyme (ACE) I/D polymorphisms with regards to the susceptibility of patients to carpal tunnel syndrome. PMID: 28370589
    43. no correlation was found between the ACE and MTHFR polymorphisms in the development of T2DM. PMID: 29694640
    44. The ACE Ins/Del polymorphism was associated with body composition. Ins/Ins individuals had higher phase angle (PhA) and body cellular mass index (BCMI) values. Ins/Ins individuals displayed higher PhA and BCMI values only if norm-hydrated, while they showed values similar to Del carriers if dehydrated. PMID: 28315876
    45. The DD genotype for ACE was independently associated (b = -0.44, P = 0.007) with Alzheimer's disease patients in the island of IKARIA, Greece. PMID: 28379521
    46. The ACE I/D polymorphism (rs4340) may contribute to the genetic susceptibility of community-acquired pneumonia (CAP) in Egyptian children. The ACE D allele and DD genotype were associated with higher serum ACE levels among studied CAP patients. PMID: 29028160
    47. ACE indel polymorphism is a risk factor for allergic rhinitis. (Meta-analysis) PMID: 28886328
    48. the ACE DD genotype may increase the risk of renal scar in children with vesicoureteral reflex [meta-analysis] PMID: 27506878
    49. The AA and GA genotypes of MTHFD1 G1958A, TT and GT genotypes of eNOS G894T and the AA and GA genotypes of ACE A2350G are risk factors for congenital heart defects. PMID: 28865601
    50. The ACE D allele in aMCI patients may increase the risk of cognitive impairment. A high serum ACE level possibly plays an important role in the incidence of aMCI. PMID: 28870562

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  • 相关疾病：
    Ischemic stroke (ISCHSTR); Renal tubular dysgenesis (RTD); Microvascular complications of diabetes 3 (MVCD3); Intracerebral hemorrhage (ICH)
  • 亚细胞定位：
    [Angiotensin-converting enzyme, soluble form]: Secreted.; Cell membrane; Single-pass type I membrane protein. Cytoplasm.
  • 蛋白家族：
    Peptidase M2 family
  • 组织特异性：
    Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • 数据库链接：

    HGNC: 2707

    OMIM: 106180

    KEGG: hsa:1636

    STRING: 9606.ENSP00000290866

    UniGene: Hs.298469