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  4. AGT Recombinant Monoclonal Antibody

AGT Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA263831A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA263831A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    AGT is an α2-globulin precursor of all angiotensin peptides. As a non-inhibitory serpin, AGT plays an important role in blood pressure regulation by transporting angiotensin peptides. AGT works as a substrate in the renin–angiotensinogen system (RAS) and is cleaved by the highly-specific aspartyl-protease renin at its N-terminal extension. The N-terminus is cleaved to produce angiotensin-I, a decapeptide that is then processed to produce the sub-peptides that govern blood pressure by influencing salt retention and vasoconstriction.

    Compared with the polyclonal and monoclonal antibodies of AGT, this AGT recombinant antibody has the features of increased reproducibility and control, animal-free technology, high degree of monovalency, high batch-to-batch consistency, easier isotype conversion, etc. And it has been validated in ELISA, IHC.

  • Uniprot No.：
    P01019
  • 基因名：
  • 别名：
    Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin-1 (Angiotensin 1-10) (Angiotensin I) (Ang I), Angiotensin-2 (Angiotensin 1-8) (Angiotensin II) (Ang II), Angiotensin-3 (Angiotensin 2-8) (Angiotensin III) (Ang III) (Des-Asp[1]-angiotensin II), Angiotensin-4 (Angiotensin 3-8) (Angiotensin IV) (Ang IV), Angiotensin 1-9, Angiotensin 1-7, Angiotensin 1-5, Angiotensin 1-4], AGT, SERPINA8
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Angiotensinogen
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    8A9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.; acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.; stimulates aldosterone release.; is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • 基因功能参考文献：
    1. Patients with heart failure and type 2 diabetes mellitus with AGT TT + MT genotype had a higher level of ST2 and a higher probability of unfavorable cardiovascular events during 24 months of observation compared with MM genotype carriers. PMID: 29578435
    2. Unfavorable genotype of polymorphic variants of candidate gene participating in endothelial dysfunction AGT (Thrl74Met and Met23SThr) was associated with changes in levels of their active substances in individuals exposed to mercury. PMID: 30351652
    3. Angiotensin 1-7 can modulate cell adhesion and epithelial-mesenchymal transition of normal prostate epithelial cells. PMID: 29802847
    4. These findings reveal the critical role of hypoxia in producing local angiotensin II by a lactate-chymase-dependent mechanism and highlight the importance of local angiotensin II in regulating radioresistance of hypoxic tumor cells. PMID: 28205588
    5. The reduced urinary AGT/creatinine in Autralian Indigenous pregnant women may reflect subclinical renal dysfunction which limits the ability of the kidney to maintain sodium balance and could indicate an increased risk of pregnancy complications and/or future renal disease. PMID: 29674190
    6. ACE2 and other enzymes can form ANG-(1-7) directly or indirectly from either the decapeptide ANG I or from ANG II. [review] PMID: 29351514
    7. The ACE and AGT gene polymorphisms are not associated with the progress of diabetes developing into retinopathy in Chinese patients with type 2 diabetes. PMID: 29378484
    8. AGT M235T and T174M variants contribute to an increased risk of developing preeclampsia (PE), and for M235T to PE severity. PMID: 29366364
    9. Data, including data using network analysis, suggest that angiotensinogen (AGT), mitogen-activated protein kinase-14 (MAPK14), and prothrombin (F2) in placental villous tissues are core factors in early embryonic development; these studies involved proteomics and bioinformatics analysis of altered protein expression in placental villous tissue from early recurrent miscarriage patients in comparison to control tissues. PMID: 29277264
    10. The AGT (M235T) gene polymorphism do not seem to have a significant effect on the development of clinical properties or cardiovascular comordities of acromegalic patients. PMID: 28712073
    11. Angiotensin II has a role in increasing glomerular permeability by beta-arrestin mediated nephrin endocytosis PMID: 28004760
    12. After donor nephrectomy, increasing uAGT levels can be the result of activation of the intrarenal renin-angiotensin system affecting the compensatory changes in the remaining kidney. PMID: 28988519
    13. M235T polymorphism of the AGT gene seems unrelated to the development or the clinical course of endometriosis. PMID: 29303211
    14. AGT missense polymorphisms are not associated with diabetic nephropathy in our subset of Slovenian type 2 diabetes mellitus patients PMID: 28488548
    15. Association of AGT single nucleotide polymorphism rs3789678 and gestational hypertension in Chinese population. PMID: 27910864
    16. Data suggest that angiotensin-II plays important role in development of albuminuria, particularly in subjects with impaired glucose metabolism; here, serum angiotensin-II levels are up-regulated in subjects with prediabetes and type 2 diabetes as compared to subjects with normal glucose metabolism; elevated serum angiotensin-II level may be early biomarker or risk factor for vasculitis. PMID: 28797632
    17. The T allele of AGT may play a role in the pathogenesis of preeclampsia in South African Black women. PMID: 28641212
    18. These data indicate that Ang II-AT2R regulates human bone marrow MSC migration by signaling through the FAK and RhoA/Cdc42 pathways. PMID: 28697804
    19. Data suggest that up-regulaton of Ang-(1-7) levels in follicular fluid correlates with increases in number of mature oocytes retrieved upon ovarian stimulation in preparation for in vitro fertilization. PMID: 28402544
    20. Urinary angiotensinogen and renin excretion are elevated in CKD patients. Both parameters are negatively associated with eGFR and these associations are independent of urinary albumin excretion PMID: 28395289
    21. Reduced IL-18 serum concentration in children after HUS with no difference in its urine concentration may indicate a loss of the protective effects of this cytokine on renal function due to previously occurred HUS. PMID: 27982687
    22. Angiotensin II initiates hepatocyte epithelial-mesenchymal transition by activating the NOX-derived H2O2-mediated NLRP3 inflammasome/IL-1ss/Smad circuit. PMID: 27445100
    23. angiotensin II induced abdominal aortic aneurysm growth and inflammation is attenuated by imatinib PMID: 27085160
    24. present study has demonstrated, for the first time, that high glucose augments AGT in human RPTCs through HNF-5, which provides a potential therapeutic target for diabetic nephropathy PMID: 29053707
    25. AngII-dependent phosphorylation of LCP1 in cultured podocytes was mediated by the kinases ERK, p90 ribosomal S6 kinase, PKA, or PKC. LCP1 phosphorylation increased filopodia formation. PMID: 28768720
    26. Autosomal dominant polycystic kidney disease (ADPKD), uniquely increases urinary angiotensinogen and renin excretion despite their circulating levels being comparable with those in non-ADPKD chronic kidney disease. PMID: 28747358
    27. Quaternary interactions and supercoiling modulate the cooperative DNA binding of AGT. PMID: 28575445
    28. results show that SNPs in the Hap-I of the hAGT gene promote high-fat diet-induced binding of transcription factors GR, CEBP-beta and STAT3, which lead to elevated expression of the hAGT gene in hepatic and adipose tissues PMID: 28467442
    29. Angiotensinogen import and subsequent trafficking to the mitochondria occurs in proximal kidney tubules. PMID: 27903492
    30. Transgenic mice expressing human AGT in the subfornical organ AGT and possibly ANG I/ANG II into the cerebral ventricles. PMID: 28490451
    31. AngII could induce pulmonary injury by triggering endothelial barrier injury, and such process may be related to the dephosphorylation of Y685-VE-cadherin and the endothelial skeletal rearrangement PMID: 28119542
    32. Renin-angiotensin system transgenic mouse model suggests that renal injury in preeclampsia may be mediated through local VEGF. PMID: 27927648
    33. endoplasmic reticulum stress induces apoptosis in human alveolar epithelial cells through mediation of unfolded protein response pathways, which in turn regulate the autocrine ANGII/ANG1-7 system. PMID: 27638906
    34. Angiotensin II stimulates PYY secretion, in turn inhibiting epithelial anion fluxes, thereby reducing net fluid secretion into the colonic lumen. PMID: 27447725
    35. NOXs had two time-dependent reactions in response to Ang II stimulation via MAPK pathwa PMID: 27807688
    36. increased Ang-II/Ang-(1-7) ratios may lead to Ang-II over-activation and aggravate atherosclerosis progression. PMID: 27432541
    37. urinary angiotensinogen could be a prognostic marker of the albuminuria reduction effects of olmesartan in patients with metabolic syndrome PMID: 27801805
    38. AGT expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    39. Urinary angiotensinogen reflects intrarenal RAS status, and is of value to assess the severity of acute tubular necrosis . PMID: 27788506
    40. Data suggest that enhanced survival of normal prostate epithelial cells (PNT1A cell line) in presence of either or both angiotensin II (Ang II) and relaxin 2 (RLN2) appears to be associated with increased ratio of BCL2/BAX messenger RNA expression; thus, Ang II and/or RLN2 appear to be involved in regulation of apoptosis in prostate epithelium. PMID: 27119161
    41. increasing the serum levels of AngII increased the risk of acute myocardial infarction (AMI); the risk of AMI increased when the serum levels of AngII and KLK1 simultaneously increased; individuals with the combined genotypes of ACE DD and KLK1 GG showed increased risk of AMI compared with those with the combined genotypes of ACE II and KLK1 AA PMID: 27329205
    42. genetic association study and meta-analysis revealed a significant association between M235T polymorphism of angiotensinogen and myocardial infarction risk PMID: 27586550
    43. significant association between Thr207Met polymorphism and chronic kidney disease progression in autosomal dominant polycystic kidney disease PMID: 26482465
    44. The aim of this work is to assess the influence of the rs1799752 polymorphism of the angiotensin-converting enzyme (ACE) gene and the rs699 polymorphism of the angiotensinogen (AGT) gene on the in-stent restenosis in Polish patients with stable coronary artery disease. PMID: 27162064
    45. in cultured H9C2 cells and that CyPA/CD147 signaling pathway inhibition protects against Ang II-induced cardiomyocyte hypertrophy. PMID: 27590243
    46. The circulating ACE2 and Ang-(1-7) levels were related to neither rs4646155 nor rs879922 in female or male patients.In conclusion, the rs2106809 polymorphism of the ACE2 gene may be a determinant of the circulating Ang-(1-7) level in female patients with hypertension, suggesting a genetic association between circulating Ang-(1-7) levels and ACE2 gene polymorphisms in patients with hypertension. PMID: 27310975
    47. observed significantly higher prevalence of ACE DD and AGTR1 1166CC genotypes in hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) patients. Also, 235TT genotype of AGT (M235T) was significantly associated with enhanced risk of the disease phenotype in HCM, DCM, and RCM. PMID: 28120210
    48. Angiotensinogen expression is down regulated in human ileal Crohn's disease, particularly in the presence of inflammation and current cigarette smoking, implicating the mesenteric vasculature and mucosal hypoxia as co-factors in ileal CD pathogenesis. PMID: 27014752
    49. AGT M235T polymorphism was associated with SAH risk in Caucasian-Brazilians, and no association was detected with CAD PMID: 27019433
    50. Protective role of g.5855G>A polymorphism in the development of Essential Hypertension in Indian population was reported. The results reflect possibility of ethnic variation in contribution of g.5855G>A polymorphism of the AGT gene to the disease. PMID: 27398822

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  • 相关疾病：
    Essential hypertension (EHT); Renal tubular dysgenesis (RTD)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    HGNC: 333

    OMIM: 106150

    KEGG: hsa:183

    STRING: 9606.ENSP00000355627

    UniGene: Hs.19383