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  4. CD99 Recombinant Monoclonal Antibody

CD99 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA004973A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in Jurkat whole cell lysate
      All lanes CD99 antibody at 0.8μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 28 KDa
      Observed band size: 28 KDa
    • IHC image of CSB-RA004973A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Overlay histogram showing Jurkat cells stained with CSB-RA004973A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    CD99 antibody CSB-RA004973A0HU is a recombinant monoclonal antibody belonging to rabbit IgG. Its production procedures include: the acquisition of the CD99 monoclonal antibody using the synthesized peptide derived from the human CD99 immunizes animals; the determination of DNA sequence of the CD99 monoclonal antibody; the clone of the DNA sequence into the plasmid and subsequent transfection into cell lines for expression. This CD99 antibody underwent purification using affinity chromatography. It can detect human CD99 in ELISA, WB, IHC, and IP.

    CD99, also known as MIC2, is a transmembrane protein that is extensively O-glycosylated and found on leukocytes and active endothelium. CD99 regulates cell adhesion and migration, cell death and differentiation, intracellular protein trafficking, endocytosis, and exocytosis, among other important biological processes. CD99, in particular, functions as an oncosuppressor in tumors or as a prerequisite for cell malignancy. CD99 has been shown to have an impact on tumor cell differentiation, migration, invasion, and metastasis. CD99 is a diagnostic marker for Ewing's Sarcoma (EWS) since these tumors highly express this protein.

  • Uniprot No.：
    P14209
  • 基因名：
  • 别名：
    CD99; MIC2; MIC2X; MIC2Y; CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD antigen CD99
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9A5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.
  • 基因功能参考文献：
    1. Besides confirming the importance of CD99, findings indicate that polymorphic variations in this gene may affect either development or progression of EWS. PMID: 27792997
    2. CD99 is a marker of acute myeloid leukemia and myelodysplastic syndrome stem cells PMID: 28123069
    3. CD99 expression is increased in patients with active Inflammatory bowel disease, and positively correlated with disease activity. PMID: 27866429
    4. CD99 counteracts EWS-FLI1 in controlling NF-kappaB signaling through the miR-34a, which is increased and secreted into exosomes released by CD99-silenced Ewing sarcoma cells. PMID: 26616853
    5. these observations suggest that CD99 is involved in the regulation of CD1a transcription and expression by increasing ATF-2. PMID: 27094031
    6. CD99 was also paranuclear positive in 4 of 11 (36%) small cell lung carcinomas PMID: 26782152
    7. High expression of EMMPRIN plays a crucial role in breast cancer cell proliferation, matrigel invasion and tumor formation. PMID: 27002769
    8. Data show that CD99 antigen expression increased during in vitro differentiation of germinal center B cells and was highest in plasma cells (PCs). PMID: 26522646
    9. Our results suggest that the CD34/CD25/CD123/CD99(+) LAIP is strictly associated with FLT3-ITD-positive cells. PMID: 25957287
    10. The results highlight an important role of CD99 in the differentiation and activity of human osteoblasts in physiological and pathological conditions. PMID: 26000312
    11. CD99 can suppress CD98-mediated assembly of pro-tumorigenic signaling complexes through its ability to dephosphorylate focal adhesion kinase. PMID: 26172215
    12. CD99 expression in astrocytomas of different malignant grades might contribute to the infiltrative ability and support the importance of CD99 as a potential target to reduce infiltrative astrocytoma capacity in migration and invasion. PMID: 24797829
    13. The dAbd C7 induced rapid and massive EWS cell death through Mdm2 degradation and p53 reactivation. Mdm2 overexpression as well as silencing of p53 in p53wt EWS cells decreased CD99-induced EWS cell death PMID: 25501132
    14. CD99 expression in nasal lobular capillary haemangioma. PMID: 24702676
    15. dermatofibromastrongly expresses CD99 in a diffuse pattern that may serve as evidence in distinction from dermatofibrosarcoma PMID: 24247571
    16. CD99 induces ERK activation, increasing its membrane-bound/cytoplasmic form rather than affecting its nuclear localization. PMID: 24677094
    17. The new antibody scFvC7 recognizes the CD99 extracellular domain. PMID: 22335486
    18. Report purification of human antibody scFv anti-CD99 for blocking monocyte transendothelial migration. PMID: 24798881
    19. Solid pseudopapillary neoplasm of the pancreas exhibits a very unique immunostaining pattern for CD99 that is present in all cases. PMID: 24094957
    20. we have shown that forced expression of wild-type CD99 in osteosarcoma cells induces downregulation of genes such as ROCK2 PMID: 23644663
    21. CXCL16, inversely correlated with CD99 expression in Hodgkin Reed-Sternberg (H/RS) cells. PMID: 23743627
    22. It is assumed that the expression of CD99 plays an important role in the pathogenesis of psoriasis, in the processes of emigration of leukocytes, and in their tropism toward to the epidermis PMID: 24341229
    23. we show that miR-30a-5p provides an essential link between EWS-FLI1 and CD99, two major biomarkers in Ewing sarcoma PMID: 22986530
    24. CD99 is highly expressed in anaplastic large-cell lymphoma, and showed high rate of co-expression with ALK. PMID: 22781601
    25. PVR resides in the recently identified lateral border recycling compartment, similar to PECAM and CD99. PMID: 23333754
    26. We report an unusual pattern of paranuclear dot-like expression of CD99 in 14 cases of Merkel cell carcinoma, two of which did not express CK20. PMID: 23145531
    27. CD99-induced HSP70 expression in B and T lymphocytes. PMID: 23152061
    28. Data suggest that CD99 expression in lymph node/germinal center biopsies at time of diagnosis is not prognostic marker for survival in patients with diffuse large B-cell lymphoma treated with rituximab-CHOP immunochemotherapy. PMID: 22864685
    29. the membrane protein CD99 was identified as a novel stromal factor with clinical relevance. The results support the concept that stromal properties have an important impact on tumour progression. PMID: 22392539
    30. upregulation of CD99 in H/RS cells induces terminal B-cell differentiation, which may provide a novel therapeutic strategies for cHL PMID: 22020966
    31. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) and CD99 are critical in lymphatic transmigration of human dendritic cells. PMID: 22189791
    32. NF-kappaB2 exhibits the major inhibitory role in the transcription at the CD99 promoter PMID: 22083306
    33. High CD99 is associated with central primitive neuroectodermal tumors and Ewing's sarcoma PMID: 21267687
    34. Data show that CD99 combined with E-cadherin/beta-catenin and CD10 can be used as a relatively specific expression profile of SPTs. PMID: 21775056
    35. Our study has identified for the first time that pancreatic solid-pseudopapillary neoplasm exhibits a unique dot-like staining pattern for CD99. PMID: 21566515
    36. CD99 positivity was significantly associated with advanced stage (p < 0.01), higher risk group according to the International Prognostic Index risk score (p < 0.01) and non-germinal center B cell-like type (p = 0.01). PMID: 21196719
    37. Protein expression and gene promoter hypermethylation of CD99 in transitional cell carcinoma of urinary bladder PMID: 20217126
    38. CD99 showed no preferential staining of Atypical fibroxanthoma, spindle cell malignant melanoma or sarcomatoid carcinoma/spindle cell squamous cell carcinoma PMID: 20184665
    39. CD99 may play a physiologic role in the clonal deletion processes necessary for B-lymphoid selection PMID: 20453109
    40. Analysis of a panel of human EWS cells revealed an inverse correlation between CD99 and H-neurofilament expression, as well as an inverse correlation between neural differentiation and oncogenic transformation. PMID: 20197622
    41. Results suggest that type I is the major isoform of CD99 expressed in non-neoplastic gastric mucosa and gastric adenocarcinomas. PMID: 12172043
    42. Functional involvement of src and focal adhesion kinase in a CD99 splice variant-induced motility of human breast cancer cells PMID: 12216109
    43. engagement triggers the exocytic transport of ganglioside GM1 and the reorganization of actin cytoskeleton PMID: 12681511
    44. inhibition by MHC class I engagement or apoptosis and upregulation of T cell receptor and MHC molecules in human thymocytes and T cell line induced by CD99 PMID: 12832073
    45. CD99 epitopes plays a distinct role in T cell biology, especially in T cell apoptosis. PMID: 14623115
    46. CD99 type II acts as a negative regulator in the neural differentiation of precursor cells that might occur during nerve system development PMID: 14646598
    47. Solitary sclerotic fibroma is a fibrotic lesion with cells positive for CD34 and O13. O13 expression in SF has not been previously described. PMID: 14744088
    48. our findings indicate that CD99 functions occur through reorganization of cytoskeleton and identify actin and zyxin as the early signaling events driven by CD99 engagement. PMID: 15184883
    49. solution structure of cytoplasmic domain of human CD99 type I shows that it has a hairpin shape anchored by two flexible loops PMID: 15359120
    50. cyclophilin A binds CD99 and may be either a signaling mediator or a signaling regulator for CD99 PMID: 15388255

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    CD99 family
  • 数据库链接：

    HGNC: 7082

    OMIM: 313470

    KEGG: hsa:4267

    STRING: 9606.ENSP00000370588

    UniGene: Hs.653349