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  4. FCGR2A Recombinant Monoclonal Antibody

FCGR2A Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA163349A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: THP-1 whole cell lysate
      All lanes: CD32 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 35, 36 kDa
      Observed band size: 45 kDa
  • 其他：

产品详情

  • 产品描述：

    FCGR2A, also called CD32, is the FcγRII immunoglobulin receptor that controls the adaptive immune response and plays a role in the clearance of immune complexes by macrophages, neutrophils, and platelets. It is mainly involved in binding with IgG. FCGR2A gene polymorphism was reportedly associated with the susceptibility to inflammation-related diseases, such as atherosclerosis, Takayasu arteritis, systemic lupus erythematosus (SLE), and ulcerative colitis. Alberici et al. reported that FCGR2A was a gene hallmark for the autoimmunity among the patients with chronic periaortitis.

    To produce this recombinant FCGR2A antibody, we needed to get the gene sequence of the antibody.  B cell screening was used in the process. Once the sequence was obtained, it would be lead to the expression plasmids so that the FCGR2A antibody can be expressed in mammalian cells.  Moreover, this recombinant FCGR2A antibody was validated in ELISA, WB.

  • Uniprot No.：
    P12318
  • 基因名：
  • 别名：
    FCGR2A; CD32; FCG2; FCGR2A1; IGFR2; Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD antigen CD32
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CD32
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3B6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
  • 基因功能参考文献：
    1. The results demonstrate that CD32 expression is a marker of CD4+ T cell activation in HIV+ individuals. PMID: 30013105
    2. this study shows that genetic variation of human neutrophil Fcgamma receptors and SIRPalpha in antibody-dependent cellular cytotoxicity towards cancer cells PMID: 28952147
    3. this study shows that FCGR genetic polymorphism affects antibody responses to GARP in patients with breast cancer PMID: 29879453
    4. Data indicate that the Fc gamma receptor FCgammaRIIA polymorphisms did not significantly influence response to rituximab in immune thrombocytopenic purpura (ITP). PMID: 28856973
    5. Results showed significantly higher frequency of FC gamma receptor FCGR3A-158V allele in patients with immune thrombocytopenia (ITP) compared with control subjects, but not significant differences in the genotype distribution or allele frequencies for FC gamma receptor FCGR2A-131H/R between patients and controls. PMID: 28942727
    6. Differently oxidized isolated subspecies can lead both to stronger as well as weaker binding and activation of the histidine variant of Fc fragment of IgG receptor IIa (FcgammaRIIa). PMID: 28988621
    7. CRP bound to surface CD32 (also known as FcgammaRII) on myeloma cells, which activated a pathway mediated by the kinase p38 MAPK and the transcription factor Twist that enhanced the cells' secretion of osteolytic cytokines. PMID: 29233917
    8. FCGR2A single nucleotide polymorphism confers susceptibility to idiopathic nephrotic syndrome PMID: 29155175
    9. alphaIIb beta3 antagonist TMV-7/trimucrin prevents thrombosis with causing Fc receptor gamma-chain IIa-mediated thrombocytopenia. PMID: 28815933
    10. The expression levels of human FcgammaRIIB but not FcgammaRIIA were negatively correlated with serum levels of IgE in human asthma patients. PMID: 29597194
    11. When higher-affinity genotypes for FCGR2A, FCGR3A, and FCGR2C were considered together, they were associated with significantly increased tumor shrinkage and prolonged survival in response to HD-IL2... this is the first study to show associations of FCGR genotypes with outcome following HD-IL2 treatment PMID: 27742794
    12. FcgammaRIIA and FcgammaRIIB both demonstrated increased methylation levels in Kawasaki disease (KD) patients that underwent IVIG treatment. FcgammaRIIA expression influenced the IVIG treatment response of KD patients. The FcgammaRIIA/IIB mRNA expression ratio was greater in KD patients with coronary artery lesion formation. PMID: 27893416
    13. FCGR2A rs1801274 G-allele confers susceptibility to Kawasaki disease and Ulcerative colitis PMID: 27270653
    14. farletuzumab has enhanced binding to FCGR3A-158V high-affinity receptor and has an enhanced clinical outcome in ovarian cancer patients with low baseline CA125 levels and at least 1 high-affinity allele of FCGR2A or FCGR3A PMID: 29041009
    15. inhibition of Abl/Src with bosutinib reduced FcgammaRIIA-mediated glomerular neutrophil accumulation and renal injury in experimental, crescentic anti-GBM nephritis. PMID: 28891817
    16. The present study demonstrates that p.His167Arg, a KD-associated FCGR2A variant, acts as a susceptibility gene in males only. Overall, the gender differences associated with FCGR2A in KD provide a new insight into KD susceptibility. PMID: 28886140
    17. Data indicate a mechanism in which Toll-like receptors TLR7/8 signaling, through shedding of FcgRIIA, shifts neutrophil function from phagocytosis to a programmed necrosis pathway, neutrophil extracellular trap formation (NETosis). PMID: 28606989
    18. Gene copy number variation (CNV) of the PKLR, FCGR2A, FCGR2C, and FCGR3 genes is associated with malaria severity, and our results provide evidence for a role of CNV in host responses to malaria. PMID: 28605553
    19. This study demonstrated that no association between FCGR2A polymorphisms in Guillain-Barre Syndrome in a Brazilian population. PMID: 27609290
    20. FCGR3A V and FCGR2A R allele carriers show better responsiveness to anti-TNF-alpha therapy--{REVIEW} PMID: 27490376
    21. Association between Fc gamma receptor IIA genetic polymorphisms and susceptibility to severe malaria anemia in children in western Kenya. PMID: 28427365
    22. identification of a subpopulation of 0.012% of CD4 T cells that express CD32a and host up to three copies of HIV DNA per cell; this CD32a(+) reservoir was highly enriched in inducible replication-competent proviruses and can be predominant in some participants; that CD32a(+) lymphocytes represent the elusive HIV-1 reservoir may lead to insights that will facilitate the specific targeting and elimination of this reservoir PMID: 28297712
    23. Single nucleotide polymorphisms (SNPs) rs2099684 in IgG receptors FCGR2A/FCGR3A can be considered a genetic risk factor for Takayasu arteritis (TA) in the Chinese Han population. PMID: 27769046
    24. The mutant homozygote (CC) of the FCGR2A gene (rs1801274) may have a protective role among Chinese patients with UC. PMID: 27984611
    25. Review/Meta-analysis: FcgammaRIIa-H131R may modify treatment rituximab response in diffuse large B cell lymphoma. PMID: 28039707
    26. FCGR2A and FCGR2C polymorphisms may also contribute to immunocomplexemia present in sarcoidosis. PMID: 26801149
    27. The absence of TULA-2 and also the relative level of TULA-2 expression modulates FcgammaRIIA-mediated platelet reactivity and heparin-induced thrombocytopenia in vivo. PMID: 27765766
    28. FCGR2A expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    29. Review/Meta-analysis: Rheumatoid arthritis patients with FCGR2A HH + HR genotype show a poor response to adalimumab. PMID: 27074847
    30. Our findings indicate that CD16 158F>V polymorphism may contribute to the increased risk of Idiopathic Thrombocytopenic Purpura, whereas CD32 131H>R polymorphism may not be an important risk factor for Idiopathic Thrombocytopenic Purpura. PMID: 27315784
    31. Our observations support the existence of a central FcgammaRIIA-mediated pathway by which human platelets respond to both Gram-negative and Gram-positive bacteria. PMID: 27025455
    32. FcgammaRIIA H131 allele and FcgammaRIIA H/H131 genotype were significantly increased in pediatric Guillain-Barre syndrome patients. PMID: 27064330
    33. FCGR2A/FCGR3A-related immune disorder might contribute to the etiology of Takayasu arteritis. PMID: 26996483
    34. Data indicate no significant difference in the allele or genotype frequencies of the Fcgamma2RA protein (FCGR2A) rs1801274 single nucleotide polymorphism was observed between groups. PMID: 27267995
    35. The RR, HR, and HH FCGR2A-131 genotypes were detected in 1 (11 %), 5 (56 %), and 3 (33 %) of patients with disease relapse compared to 25 (21 %), 56 (47 %), and 38 (32 %) of the 119 patients without relapse. PMID: 27376362
    36. The data indicate that FcgammaRIIA genotyping can be used as a marker of genetic susceptibility to sepsis. PMID: 26490967
    37. FCGR3A and FCGR2A SNPs do not confer differential responsiveness to rituximab. PMID: 26510856
    38. Fc-gamma receptor polymorphisms differentially influence susceptibility to systemic lupus erythematosus and lupus nephritis. PMID: 26748351
    39. R/R genotype of FCGR2A p.R131H and G/G genotype of CCL2 c.-2518 A > G polymorphisms are associated with thrombocytopenia, which is a characteristic laboratory finding in dengue infections. PMID: 26429304
    40. Genetic variants of rs6671847 at FCGR2A and rs17085007 at 13q12 conferred a risk of relapse in patients with ulcerative colitis. PMID: 25787843
    41. we fail to find the significant association between FCGR2A H131R and clinical outcome in KRAS wild metastatic colorectal cancer individuals with adjuvant cetuximab therapy PMID: 26363448
    42. The results show an association between FcgRIIa, TNF-alpha and IL-6 gene single nucleotide polymorphisms and symptom persistence in Dengue patients. PMID: 26429310
    43. FCGR2A polymorphisms constitute a risk factor for graft loss following kidney transplantation and that this effect is related to anti-HLA antibodies. PMID: 26429312
    44. FcgammaRIIIA/FcgammaRIIA gene polymorphisms and HER-2 may have a role in antibody-dependent cellular cytotoxicity and clinical response to trastuzumab in breast cancer PMID: 26450443
    45. The goal of the study was the development of a novel method for Y402H (g.43097C>T) genotyping, the confirmation of its association with AMD in the Greek population and the investigation of the H131R polymorphism in AMD PMID: 25811666
    46. These include an FCGR2A/2C chimeric gene that causes a decreased expression PMID: 26133275
    47. Genetic variation Fc gamma receptor IIA may contribute to infectious susceptibility in trauma patients. PMID: 26496101
    48. this meta-analysis suggested that the H131R polymorphism in the FCGR2A gene might be associated with susceptibility to KD in Asians. PMID: 26125827
    49. study found homozygous carriers of the FcgammaRIIA-131R/R allele had higher malaria-specific antibody levels compared to heterozygous carriers of FcgammaRIIA-131R/H alleles and to homozygous carriers of FcgammaRIIA-131H/H alleles; the pre-existing antibody responses were related to reduced subsequent risk of clinical malaria PMID: 25447268
    50. Review: discuss role of human FCGR2A in immune processes and thrombosis. PMID: 25900780

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Found on monocytes, neutrophils and eosinophil platelets.
  • 数据库链接：

    HGNC: 3616

    OMIM: 146790

    KEGG: hsa:2212

    STRING: 9606.ENSP00000271450

    UniGene: Hs.352642