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  4. FDFT1 Recombinant Monoclonal Antibody

FDFT1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA909932A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate
      All lanes: FDFT1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 49, 41, 39, 36, 44 kDa
      Observed band size: 50 kDa
    • IHC image of CSB-RA909932A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA909932A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    FDFT1 is a membrane-associated enzyme that plays a critical role in directing intermediates produced from mevalonate to either a non-sterol pathway or cholesterol synthetic pathway. It catalyzes a two-step reaction in which squalene is synthesized via the condensation of two molecules of farnesyl pyrophosphate (FPP). Accumulating evidence has noted that FDFT1 plays a critical role in cancer, particularly in metabolic reprogramming, cell proliferation, and invasion. FDFT1 is involved in the propagation of some viruses of the Flaviviridae family. Zaragozic acid A, an inhibitor of FDFT1, could inhibit dengue virus replication. Studies have demonstrated that FDFT1 is the key regulator of HCV propagation and may contribute to HCV-mediated pathogenesis.

    The generation of this recombinant FDFT1 antibody occurs in a series of steps: immunization, splenocytes & PBMC, single B cell sorting, mRNA extraction, RT-PCR & insert vector, expression, ELISA validation. And ELISA, WB, IHC was carried out Every step was performed under strict standards to ensure the researchers can have a recombinant FDFT1 antibody with premium quality.

  • Uniprot No.：
    P37268
  • 基因名：
    FDFT1
  • 别名：
    Squalene synthase (SQS) (SS) (EC 2.5.1.21) (FPP:FPP farnesyltransferase) (Farnesyl-diphosphate farnesyltransferase), FDFT1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human FDFT1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    10H12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
  • 基因功能参考文献：
    1. The results indicate that the ennea-peptide CLSPHSMFC, tetra-peptides SMFC, CKTE, and WHQW can effectively inhibit Squalene Synthase activities (IC50 values equal to 64, 76, 87, and 90 muM, respectively) PMID: 26922723
    2. The minor allele in FDFT1 was associated with advanced fibrosis in the non-steatotic but not in the steatotic subgroup in patients with chronic hepatitis C. PMID: 23870067
    3. Up-regulation of SQS promotes metastasis of lung cancer by enhancing tumor necrosis factor-alpha receptor 1 and nuclear factor-kappaB activation and matrix metallopeptidase 1 expression. PMID: 25152164
    4. Several critical residues that are involved in binding of NADPH have been identified in the active site of squalene synthase. PMID: 24531458
    5. HCV infection increases FDFT1 protein level but not FDFT1 mRNA level. PMID: 24690320
    6. this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs. PMID: 24333579
    7. ABHD5, PGRMC1 and SQS are novel markers for sebaceous carcinoma and can reliably distinguish sebaceous neoplasms from non-sebaceous tumors, specifically BCC with clear cell features. PMID: 23557589
    8. FDFT1 and its encoded enzyme, squalene synthase, may play an important role in prostate cancer development and its aggressive phenotypes. PMID: 22546838
    9. CTSB and FDFT1 are excluded as candidates for keratolytic winter erythema PMID: 21945151
    10. NASH is intimately related to fibrosis progression, the FDFT1 SNP is a good candidate for a link with inflammation and fibrosis. PMID: 21439984
    11. In multivariate models adjusted for age, body mass index, diabetes, waist/hip ratios, and levels of glycated hemoglobin, the NAFLD activity score was associated with the SNP rs2645424 on chromosome 8 in farnesyl diphosphate farnesyl transferase 1 (FDFT1) PMID: 20708005
    12. Results report the high-resolution x-ray structures of human farnesyl pyrophosphate synthase in complexes with risedronate and zoledronate, two of the leading nitrogen-containing bisphosphonates in clinical use. PMID: 16684881
    13. in prostate cancer cells SQS expression is enhanced by androgens, channeling intermediates of the mevalonate/isoprenoid pathway toward cholesterol synthesis PMID: 17483544
    14. lysine in codon 45 is conserved across 11 mammals and lies in an exonic splicing enhancer site, suggesting this coding variant in the squalene synthase gene influences plasma cholesterol levels, by affecting the intracellular production of cholesterol PMID: 18350552
    15. the liver X receptor alpha directly silencing the expression of two key cholesterologenic enzymes (lanosterol 14alpha-demethylase (CYP51A1), and squalene synthase (farnesyl diphosphate farnesyl transferase 1)) via novel negative LXR DNA response elements PMID: 18676367
    16. squalene synthase may be involved in the etiology of hypercholesterolemia PMID: 19054015
    17. FDFT1 rs7001819 showed no association with obesity, neither when analysing quantitative traits nor when performing case-control studies of obesity PMID: 19245693

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Phytoene/squalene synthase family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 3629

    OMIM: 184420

    KEGG: hsa:2222

    STRING: 9606.ENSP00000220584

    UniGene: Hs.593928