Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 重组抗体
  4. FNTB Recombinant Monoclonal Antibody

FNTB Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA267691A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue
      All lanes: FNTB antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 49, 44 kDa
      Observed band size: 49 kDa
    • IHC image of CSB-RA267691A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunoprecipitating FNTB in K562 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-RA267691A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-RA267691A0HU(2µg)+ K562 whole cell lysate(500µg)
      Lane 3: K562 whole cell lysate (10µg)
  • 其他：

产品详情

  • 产品描述：

    The production of the recombinant FNTB antibody depended on Single B Cell technology. There are 3 main steps in the production: 1, Isolation of single B cells. High-throughput methods could be used to obtain the efficient identification and desired specificity of B cells. 7, Single B cell antibody sequencing and cloning. In this step, the antibody gene sequence of FNTB was obtained and introduced to plasmids, which then would be transferred to mammalian cells for in vitro expression of the FNTB antibody. 3, Screening of antibodies. The target antibody was obtained in this step. And it has been validated in ELISA, WB, IHC, IP.

    FNTB is a protein-coding gene that encodes the protein farnesyltransferase subunit beta. The related pathways are G-protein signaling_H-RAS regulatory pathway and G-protein signaling_RhoB regulatory pathway. According to some studies, FNTB may have the following features.
    Overexpression of FNTB and activation of Ras induces hypertrophy and promotes cardiomyocyte apoptosis and autophagic cell death. The lack of FNTb interfered with the conversion of prelamin A to mature lamin A, resulting in a significant accumulation of unarylated prelamin A on Western blot.

  • Uniprot No.：
    P49356
  • 基因名：
    FNTB
  • 别名：
    Protein farnesyltransferase subunit beta (FTase-beta) (EC 2.5.1.58) (CAAX farnesyltransferase subunit beta) (Ras proteins prenyltransferase subunit beta), FNTB
  • 反应种属：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human FNTB
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    5H4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IP 1:200-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.
  • 蛋白家族：
    Protein prenyltransferase subunit beta family
  • 数据库链接：

    HGNC: 3785

    OMIM: 134636

    KEGG: hsa:100529261

    STRING: 9606.ENSP00000246166

    UniGene: Hs.325531