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  4. GABRA5 Recombinant Monoclonal Antibody

GABRA5 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA559038A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Ntera-2 whole cell lysate, HEK293 whole cell lysate
      All lanes: GABA A Receptor alpha 5 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 53 kDa
      Observed band size: 70 kDa
    • IHC image of CSB-RA559038A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    The GABRA5 monoclonal antibody's DNA sequence was inserted into the plasmid, which was subsequently transfected into the cell line for expression. The GABRA5 recombinant monoclonal antibody was produced after purification using affinity chromatography. This rabbit IgG GABRA5 recombinant antibody has been evaluated in scientific applications such as ELISA, WB, and IHC. It exclusively responds with GABRA5 from humans.

    GABRA5 is abundantly expressed in the hippocampus, mainly in the extrasynaptic area of CA1 pyramidal cells, where it regulates tonic inhibitory conductance and may cause synaptic plasticity and memory impairments. GABRA5-mediated phasic inhibition via vasoactive intestinal peptide (VIP) input to interneurons is important for anxiety modulation while the GABRA5 tonic suppression through this subunit may regulate spatial learning.

  • Uniprot No.：
    P31644
  • 基因名：
    GABRA5
  • 别名：
    Gamma-aminobutyric acid receptor subunit alpha-5 (GABA(A) receptor subunit alpha-5), GABRA5
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human GABA A Receptor alpha 5
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    5C11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Ligand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapse.
  • 基因功能参考文献：
    1. Low GABRA5 expression typified hyperproliferative tumors, and loss of taurine signaling correlated with reduced patient survival, suggesting this tumor suppressive mechanism operates in vivo. PMID: 29787571
    2. Study found a significant reduction in [(11)C]Ro15 4513 binding in the nucleus accumbens in an opiate-dependent compared with the healthy control group. Study suggests that reduced GABA A receptor alpha5 levels in the nucleus accumbens are associated with addiction. PMID: 26876472
    3. Report benzodiazepine derivative as GABA-site inhibitor of extra-synaptic GABAA alpha5 receptors. PMID: 26169564
    4. Study found that a significant portion of GABAergic postsynaptic compartments contain the alpha5 GABAAR subunit, both in vitro and in vivo PMID: 25663431
    5. This study showed that in male groups, the expression of GABRA5 was generally lower in schizophrenia cases compared to the control. PMID: 25660468
    6. Findings provide genetic evidence for the involvement of the genes GABRB3 and GABRA5 in the susceptibility to panic disorder PMID: 24755890
    7. 1,2-dichlorohexafluorocyclobutane enhancement of GABRA5 activity is abolished by GABRB3 mutations. PMID: 25211390
    8. The alpha5 nicotinic receptor GABAergic neurons form a link from the medial habenula to the serotonergic brain centers. PMID: 24227714
    9. These results provide initial evidence of a GABAA alpha5 deficit in autism spectrum disorder and support further investigations of the GABA system in this disorder. PMID: 22546616
    10. In subjects with schizophrenia, mean GABA (A) alpha5 rerceptor subunit mRNA expression is 15% lower in layer 4 of the dorsolateral prefrontal cortex. PMID: 20843900
    11. These results suggest that the GABRA5 gene may confer susceptibility to bipolar I disorder. PMID: 15882799
    12. GABA(A) receptor alpha5 subunit as a candidate gene for autism and bipolar disorder; observations suggest parent-of-origin and gain-of-function effects PMID: 17353214

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily, GABRA5 sub-subfamily
  • 数据库链接：

    HGNC: 4079

    OMIM: 137142

    KEGG: hsa:2558

    STRING: 9606.ENSP00000335592

    UniGene: Hs.24969