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  4. GPA33 Recombinant Monoclonal Antibody

GPA33 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA245537A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    GPA33 has high homology with CD2 and possesses a V-type Ig-like domain at its N terminus. It's also linked to T cell activation inhibitory proteins including CD276 (B7-H3) and VSIG4, as well as many cell adhesion molecules like CEACAM, ICAM, and NCAM (3D-Protein BLAST, NCBI). Immune dysfunction has been linked to GPA33. Polymorphisms in the gene encoding GPA33 have been linked to the autoimmune condition eosinophilic granulomatosis with polyangiitis. Furthermore, the GPA33 gene is found in a Sjögren's Syndrome susceptibility locus. Hypersensitivity to dietary allergens and increased inflammatory responses to experimentally caused colitis was seen in a GPA33 knockout mice model.

    The preparation of the recombinant GPA33 antibody involves the mammalian cell lines expression of plasma vectors containing GPA33 antibody genes. B cells isolated from immunized animals' blood were treated to obtain RNA, which underwent reverse transcription to yield DNA genes. Antibody genes were sequenced and screened from the DNA. After transient expression, cell supernatant was collected and then purified using Affinity-chromatography to obtain the recombinant GPA33 antibody. This recombinant GPA33 antibody is recommended to use in the GPA33 for the detection of GPA33 protein from Human.

  • Uniprot No.：
    Q99795
  • 基因名：
  • 别名：
    Cell surface A33 antigen (Glycoprotein A33), GPA33
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human GPA33
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4H4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May play a role in cell-cell recognition and signaling.
  • 基因功能参考文献：
    1. A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma PMID: 28226180
    2. A33 expression is directly correlated to colorectal tumor differentiation. PMID: 27272411
    3. Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively. PMID: 23230278
    4. Data indicate positive staining was observed for both A33 expression and KRN330 binding from patients biopsied on Day 8, 9 or 14 following initial dosing of 3 mg/kg. PMID: 23294608
    5. The A33-dependent incorporation of B5 into extracellular enveloped vaccinia virions is mediated through an interaction between their lumenal domains. PMID: 22623777
    6. Increased interaction between vaccinia virus proteins A33 and B5 is detrimental to infectious extracellular enveloped virion production. PMID: 22623782
    7. cloning, chromosomal localizations, exon-intron structures and transcription start sites - target gene for the intestine-specific homeobox transcription factor, CDX1. PMID: 12114523
    8. Regulation of A33 antigen expression by GKLF. PMID: 12853980
    9. This work has demonstrated that the antigen is both highly immobile and extremely persistent-retaining its surface localization for a turnover halflife of greater than 2 days. PMID: 18236042

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
  • 数据库链接：

    HGNC: 4445

    OMIM: 602171

    KEGG: hsa:10223

    STRING: 9606.ENSP00000356842

    UniGene: Hs.651244