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  4. GSTO1 Recombinant Monoclonal Antibody

GSTO1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA009986A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate, PC3 whole cell lysate, Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
      All lanes: GSTO1 antibody at 1.25μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 28, 24, 25 KDa
      Observed band size: 28 KDa
    • IHC image of CSB-RA009986A0HU diluted at 1:125 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA009986A0HU diluted at 1:125 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-RA009986A0HU at 1:41, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    The recombinant GSTO1 antibody production commenced with the obtaining of genes encoding antibody against GSTO1. Antibody genes were obtained by sequencing and screening DNA reversely transcribed from RNA that was extracted from the B cells isolated from immunized animals. These genes were cloned into plasma vectors and subsequently transfected into a mammalian cell line for production. The product is the recombinant GSTO1 antibody. It underwent purification using affinity-chromatography from the cell culture medium. This recombinant GSTO1 antibody has been validated to detect the GSTO1 protein from Human in the ELISA, WB, IHC, IF.

    GSTO1 is a cytosolic glutathione transferase enzyme that regulates glutathionylation, calcium channel modulation, and TLR signaling. GSTO1 also catalyzes the reduction of methylated arsenic species, protein deglutathionylation, dehydroascorbic acid, and S-phenacylglutathiones. GSTO1 dysregulation has been linked to oxidative stress and inflammation, as well as the etiology of a number of illnesses and neurological conditions. GSTO1 polymorphism and overexpression are associated with a variety of pathologic conditions, including neurologic illnesses, malignancies, and inflammatory diseases. Upregulation of GSTO1 has been found in several human cancers including esophageal squamous cell carcinoma, colorectal, and urinary bladder cancer, and has also been involved in drug resistance.

  • Uniprot No.：
    P78417
  • 基因名：
    GSTO1
  • 别名：
    AA407097 antibody; AI194287 antibody; AU018802 antibody; DKFZp686H13163 antibody; EC 2.5.1.18 antibody; Glutathione S transferase omega 1 antibody; Glutathione S-transferase omega 1-1 antibody; Glutathione S-transferase omega antibody; Glutathione S-transferase omega-1 antibody; Glutathione transferase omega 1 antibody; Glutathione-dependent dehydroascorbate reductase antibody; Glutathione-S-transferase like antibody; Glutathione-S-transferase omega 1 antibody; GSTO 1-1 antibody; GSTO-1 antibody; GSTO1 1 antibody; Gsto1 antibody; GSTO1_HUMAN antibody; GSTTLp28 antibody; GSTX antibody; Gtsttl antibody; MGC94845 antibody; MMA(V) reductase antibody; Monomethylarsonic acid reductase antibody; OTTHUMP00000020429 antibody; p28 antibody; S-(Phenacyl)glutathione reductase antibody; SPG-R antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human GSTO1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    2C7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
  • 基因功能参考文献：
    1. the effect of specific GSTO1 and GSTO2 gene variants, independently and in interaction with established risk factors (smoking, obesity and hypertension) on the risk for the most aggressive renal cell carcinoma (RCC) subtype, the clear cell RCC, is reported. PMID: 30224590
    2. Results showed that the variants rs11191979, rs2164624 and rs4925 of GSTO1 provide the susceptibility toward developing arsenic-induced skin lesions in individuals exposed to high-dose inorganic arsenic in northwest China. PMID: 29323258
    3. Significant correlation between severities of skin manifestations with GSTO1 polymorphism status in arsenic-exposed population of India. PMID: 28436716
    4. GSTO1*C is linked to the pre-stage of Alzheimer's disease; rs4925 could be a genetic marker for cognitive impairment PMID: 27259244
    5. This study demonstrated that Distribution of the GSTO1 alleles and genotypes was nearly equal between the control group and SCA2 patients. GSTO1 genotypes were not associated to clinical markers in SCA2 patients. PMID: 28017238
    6. GSTO1 genetic polymorphisms are associated with the progression of HBV infection. PMID: 27221910
    7. GSTP1 and GSTO1 polymorphisms are associated with epirubicin treatment outcomes as well as with epirubicin-related toxicity. PMID: 26354850
    8. No significant association has been found between childhood Pre-B acute lymphoblastic leukemia and GSTO1 A140D and GSTO2 N142D polymorphisms. PMID: 25726706
    9. The present study provided epidemiological evidence for a significantly increased risk of UCB in ever smokers with the Ala/Ala genotype of the GSTO1 gene and the Arg/Arg genotype of the SULT1A1 gene. PMID: 25103078
    10. Our results indicate that GSTO1*C/GSTO2*G haplotype is associated with increased risk of TCC. The modifying effect of GSTO2*G/G genotype on individual susceptibility to TCC is more pronounced, when associated with smoking. PMID: 25716313
    11. This meta-analysis demonstrates that GSTO2 polymorphism may significantly increase cancer risk in Caucasian population and is associated with elevated risk of breast cancer; while GSTO1 polymorphism is not associated with cancer risk. PMID: 25300926
    12. Our study is the first to show that the frequency of GSTO1 A140D gene polymorphism in the Turkish population is similar to other Caucasian populations and that this polymorphism is not associated with susceptibility to NSCLC. PMID: 23819933
    13. Overexpression of GSTO1 is associated with esophageal squamous cell carcinoma. PMID: 25085586
    14. GSTT1 active genotype and GSTO1 Asp140Asp and GSTO2 Asp142Asp genotypes may have a prognostic/pharmacogenomic role in patients with muscle invasive bladder cancer. PMID: 24040330
    15. Genetic variants in GSTO1 are associated with increased risk for recurrent miscarriage. PMID: 24417908
    16. The common A140D genetic polymorphism in GSTO1 was found to have significant effects on the kinetics of both the deglutathionylation and glutathionylation reactions. PMID: 23888047
    17. The frequencies of GSTO1 and GSTO2 genotypes were not significantly different between head and neck squamous cell carcinoma cases and controls. PMID: 23086268
    18. Single nucleotide polymorphisms (SNPs) in arsenic methyltransferase and methylene-tetrahydrofolate reductase is associated with bladder cancer in those exposed to low concentrations of inorganic arsenic. SNPs in glutathione S-transferase omega-1 are not. PMID: 22747749
    19. GSTO-1 plays a key role in hormesis induced by low dose trichloroethylene. PMID: 21351650
    20. Our studies confirm the lack of association between GSTO1 variants and essential hypertension risk, also for two uncommon genetic variants with large functional effects in italian patients. PMID: 22283150
    21. Results suggest a role of GSTO1-1 in the inflammatory response and the apoptotic process and indicate that A140D and E208K polymorphisms could increase risk of developing inflammatory and apoptosis-related diseases in As-exposed populations. PMID: 22293942
    22. low monomethylarsonic acid is associated with increased risk for MetS among As-exposed subjects and the genetic polymorphism of GSTO1 PMID: 22440634
    23. The minor allele of GSTO1 rs4925 associates with decreased risk in familial Parkinson disease. The minor alleles of GSTO1 rs4925 and GSTO2 rs156697 SNPs associate with lower brain levels of GSTO2, but not GSTO1. PMID: 22494505
    24. the crystal structure of a human GSTO1-1 has been determined at 1.7 A resolution in complex with the reaction product ascorbic acid PMID: 22522127
    25. Genetic polymorphisms GSTO and As3MT modify arsenic metabolism as evidenced by altered urinary arsenic excretion PMID: 22339537
    26. we hypothesized that GSTO1*E155del is an uncommon genetic variant associated with Alzheimer's Disease risk. PMID: 22100662
    27. These findings supported a role of the GSTO1 rs4925 single nucleotide polymorphism in the risk of sporadic Alzheimer disease in southern Italy. PMID: 20818931
    28. Novel folding and stability defects cause a deficiency of human glutathione transferase omega 1. PMID: 21106529
    29. Has a role in the risk Urothelial carcinogenesis PMID: 21094982
    30. the distribution of the GSTO2 gene differed significantly between asthmatics and controls PMID: 20374258
    31. This study provides the allele frequencies of GSTO polymorphism in a sample consisting of 116 apparently healthy individuals of both sexes from Rome (Central Italy). PMID: 20113212
    32. it is unlikely that glutathione S-transferases GSTA2, GSTM2, GSTO1, GSTO2, and GSTZ1 participate in breast cancer susceptibility. PMID: 19859803
    33. GSTO1-1 overexpression appears to be associated with activation of survival pathways (Akt and ERK1/2) and inhibition of apoptotic pathways (JNK1), as well as protection against cisplatin-induced apoptosis. PMID: 20106899
    34. The GSTO1-140Asp/GSTO2-142Asp haplotype was associated with increased risk of COPD. PMID: 19513904
    35. polymorphisms in GSTO1 or GSTO2 do not appear to contribute to the large individual variability in arsenic metabolism or susceptibility to arsenicosis. PMID: 19635583
    36. Data describe the functional characterization of two variants of human glutathione-s-transferase omega. PMID: 12565892
    37. Arsenic metabolism associated hGSTO1-1 in the European ancestry group was more polymorphic than the Indigenous American Ancestry groups. Mexican Hispanics, could vary in the extent of polymorphism in these genes based upon the admixture. PMID: 12928150
    38. a significant association was found for GSTO1 (P=0.007) and a second transcribed member of the GST omega class, GSTO2 (P=0.005), located next to GSTO1. PMID: 14570706
    39. Our results do not confirm an association between PD and frequency of hGSTO2*N142D. PMID: 15159516
    40. 2 missense polymorphisms in exon 4 (Ala140Asp & Glu155DeltaGlu) were detected. Asp allele genotype affected CSF cholesterol, serum homocysteine. risk of vascular dementia, stroke, and early-onset Alzheimer's disease. PMID: 15623683
    41. no association either with age-at-onset in AD cases or with disease risk in the case-control cohort but haplotype analysis revealed a modest association of one haplotype with AD risk (p = 0.04 PMID: 15917099
    42. For E155del, the mutation frequency in Ovambo and Japanese subjects was 0.000 and 0.017, respectively, similar to those for other populations. As for E208K polymorphism, no mutation allele was found in Ovambo or Japanese subjects. PMID: 17484623
    43. Glutathione S-transferase omega is abundant in alveolar macrophages and airway secretions, with the levels decreased in patients with chronic obstructive pulmonary disease. PMID: 17617905
    44. The GSTO1 Asp/Asp genotype presumably modulates the severity and expansion of atherosclerosis in the circle of Willis PMID: 17717316
    45. The prevalence of the GSTO1 140A varied significantly among different regional populations in China, which showed that geography played a more important role in the population differentiation for this allele than the ethnicity/race PMID: 18400112
    46. Polymorphisms of glutathione S-transferase A1 and O1 and breast cancer among postmenopausal Danish women. PMID: 18414193
    47. These results suggest a possible effect of the GSTO1 and 2 locus on age of onset of FALS. PMID: 18427999
    48. These data establish the fact that the polymorphic deletion of Glu155 can cause GSTO1-1 deficiency in vivo. PMID: 18571971
    49. GSTO2*N142D genotype is significantly associated with high rish of childhood acute lymhpoblastic leukemia. PMID: 18941778
    50. Report GSTO1 gene polymorphisms in worldwide populations. PMID: 18986335

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    GST superfamily, Omega family
  • 组织特异性：
    Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung.
  • 数据库链接：

    HGNC: 13312

    OMIM: 605482

    KEGG: hsa:9446

    STRING: 9606.ENSP00000358727

    UniGene: Hs.190028