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  4. HDAC9 Recombinant Monoclonal Antibody

HDAC9 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA010245A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate
      All lanes: HDAC9 antibody at 1.54μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 112, 102, 66, 98, 118, 113, 61, 63, 58 KDa
      Observed band size: 160 KDa
    • IHC image of CSB-RA010245A0HU diluted at 1:154 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA010245A0HU diluted at 1:154 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-RA010245A0HU at 1:51, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    CUSABIO cloned HDAC9 antibody-coding genes into plasma vectors and then transfected these vector clones into mammalian cells using a lipid-based transfection reagent. Following transient expression, the recombinant antibodies against HDAC9 were harvested and characterized. The recombinant HDAC9 antibody was purified by affinity-chromatography from the culture medium. It can be used to detect HDAC9 protein from Human in the ELISA, WB, IHC, IF.

    HDAC9 is an enzyme responsible for the removal of acetyl moieties from the ϵ-amino groups of conserved lysine residues in the N-terminal tail of histones. HDAC9 can freely move between the nucleus and the cytoplasm and interacts with histone and non-histone substrates to facilitate tissue-specific transcriptional control. Biologically, HDAC9 regulates a wide range of physiological functions in both normal and diseased states, including cardiac muscle development, bone formation, T-regulatory cell function, neurological diseases, muscle differentiation, adipocyte differentiation, and cancer. HDAC9 overexpression is also prevalent in cancer cells, where it affects the expression and function of a number of key proteins involved in cancer development.

  • Uniprot No.：
    Q9UKV0
  • 基因名：
    HDAC9
  • 别名：
    HD 7 antibody; HD 7B antibody; HD 9 antibody; HD7 antibody; HD7B antibody; HD9 antibody; HDAC 7 antibody; HDAC 7B antibody; HDAC 9 antibody; HDAC 9B antibody; HDAC 9FL antibody; HDAC antibody; HDAC7 antibody; HDAC7B antibody; HDAC9 antibody; HDAC9_HUMAN antibody; HDAC9B antibody; HDAC9FL antibody; HDRP antibody; Histone deacetylase 4/5 related protein antibody; Histone deacetylase 7 antibody; Histone deacetylase 7B antibody; Histone deacetylase 9 antibody; Histone deacetylase 9A antibody; Histone deacetylase related protein antibody; Histone deacetylase-related protein antibody; KIAA0744 antibody; MEF2 interacting transcription repressor MITR antibody; MEF2 interacting transcription repressor protein antibody; MEF2-interacting transcription repressor MITR antibody; MITR antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human HDAC9
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1F2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.; Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • 基因功能参考文献：
    1. This study highlights HDAC9 as a mediator of cell invasion and angiogenesis in Triple negative breast cancer (TNBC) cells through VEGF and MAPK3 by modulating miR-206 expression and suggests that selective inhibition of HDAC9 may be an efficient route for TNBC therapy. PMID: 29936177
    2. the T allele of rs2107595 in HDAC9 increases the risk of stroke but that the G allele of rs2389995 decreases the risk of stroke in the Chinese population (Meta-Analysis) PMID: 28145521
    3. HDAC9 may be a new indicator for assessing chronic heart failure. PMID: 30074176
    4. HDAC9 may be involved in the process of diabetic nephropathy. PMID: 27633396
    5. HDAC9, in cooperation with BRG1 and MALAT1, mediates a critical epigenetic pathway responsible for vascular smooth muscle cells dysfunction. PMID: 29520069
    6. M4 macrophages are a possible source for HDAC9 and MMP12 expression in advanced human carotid plaques. PMID: 28343758
    7. HDAC9 is an important epigenetic factor regulating ox-LDL-induced endothelial cell apoptosis and inflammatory factor expression. PMID: 27100479
    8. decline in HDAC9c expression over time was accompanied by increased EZH2 expression. PMID: 27250566
    9. HDAC9 might contribute to lymphomagenesis by altering pathways involved in growth and survival, as well as modulating BCL6 activity and p53 tumor suppressor function. PMID: 27799148
    10. post-translational modification of Nkx3.2 employing HDAC9-PIASy-RNF4 axis plays a crucial role in controlling chondrocyte viability and hypertrophic maturation during skeletal development in vertebrates. PMID: 27312341
    11. Data show that the gene encoding the transcription factor SOX9 was identified by a global transcriptomic approach as an HDAC9 target gene. PMID: 26930713
    12. The minor allele A of SNP rs2107595 increased coronary artery disease risk and the severity of coronary atherosclerosis in a Chinese Han population. PMID: 27494404
    13. in leiomyosarcomas (LMS), this two-faced trait of MEF2 is relevant for tumor aggressiveness. Class IIa HDACs are overexpressed in 22% of LMS, where high levels of MEF2, HDAC4 and HDAC9 inversely correlate with overall survival. The knock out of HDAC9 suppresses the transformed phenotype of LMS cells, by restoring the transcriptional proficiency of some MEF2-target loci PMID: 28419090
    14. Based on this study, it is suggested that HDAC9 regulates the formation of APBs and could be a candidate for the target of ALT-cancer therapy. PMID: 27833022
    15. PC3/Tis21 associates with HDAC1, HDAC4, and HDAC9 in vivo, in fibroblast cells. PMID: 27333946
    16. HDAC9 is a target of miR-377 in oral squamous cell carcinoma. PMID: 28267394
    17. Studied HDAC9 gene's association with an increased susceptibility to acute coronary syndrome (ACS) in Chinese Han population. The results revealed a significant association of rs2240419 with ACS risk in which the A allele (P = 0.047) and the A allele carriers (AA + AG) (P = 0.037) were more likely to be in ACS group as compared to those in the control group. PMID: 27642596
    18. Downregulation of HDAC9 promotes gliomas. PMID: 27495233
    19. overexpression of HDAC9 contributes to OSCC carcinogenesis via targeting a transcription factor, MEF2D, and NR4A1/Nur77, a pro-apoptotic MEF2 target PMID: 26992905
    20. The aurora kinase A inhibited by MLN 8054 are both implicated in cell cycle progression and, thus, in cellular proliferation.Epigenetic regulators were targeted by SAHA inhibiting HDACs and by DZNep inhibiting the histone methyltransferase EZH2, which silences genes by trimethylating histone H3K27.Combinations of small molecular inhibitors act synergistically in rhabdoid tumor PMID: 27466490
    21. These results highlighting the significant correlation between TWIST and HDAC9 gene expression suggest that both genes may contribute to plaque stability in a coordinated way PMID: 26621503
    22. Polymorphisms of HDAC9 is associated with Ischemic Stroke. PMID: 26347468
    23. The results imply that HDAC9 is involved in the transcriptional regulation of human odontoblasts in vivo. PMID: 22297573
    24. HDAC9 was commonly expressed in retinoblastoma tissues and HDAC9 was overexpressed in prognostically poor retinoblastoma patients. PMID: 27033599
    25. HDAC9 promotes tumor formation of glioblastoma via TAZ-mediated EGFR pathway activation. PMID: 25760078
    26. the results of this study suggest that targeting HDACs by ST-3595 might represent as a novel and promising anti-pancreatic cancer strategy. PMID: 26084607
    27. results indicate that HDAC9 variant rs2107595 may be not associated with IS risk in southern Han Chinese PMID: 26093197
    28. Data show that miR-376a and HDAC9 expression are inversely correlated in hepatocellular carcinoma and suggest that HDAC9-mediated epigenetic modification may contribute to the down-regulation of the miR-376 cluster in hepatocellular carcinoma. PMID: 25613642
    29. Gene expression studies in peripheral blood mononuclear cells revealed increased mRNA levels of HDAC9. Analysis of human atherosclerotic plaques revealed no association between rs2107595 and specific plaque characteristics. PMID: 25388417
    30. The hydroxamic acid pan-HDAC inhibitor TSA synergistically inhibit the viability. PMID: 24562770
    31. The results elucidate the genetic etiology of lung adenocarcinoma by demonstrating that SNP rs10248565 in the HDAC9 gene may be a potential biomarker of cancer susceptibility. PMID: 24650256
    32. These results suggest that HDAC9 may be a suppressor and its downregulation might promote the progression process, especially in lung adenocarcinomas. PMID: 24427341
    33. study reports gene expression in skeletal muscle tissue of women with metabolic syndrome is enriched in inflammatory response-related genes; IL6R, HDAC9 and CD97 expression correlated negatively with insulin sensitivity; suggests a role for these 3 inflammatory genes in development of skeletal muscle insulin resistance in women PMID: 23771909
    34. These data suggest that HDAC9 variants may be selected for during cutaneous squamous cell carcinoma tumorigenesis PMID: 23784969
    35. In vivo sorafenib + HDAC inhibitor toxicity against tumors was increased. PMID: 23674352
    36. HDAC9 gene is significantly associated with large-vessel stroke risk in Chinese population. PMID: 23828597
    37. We propose that CtBP2 is an ovarian cancer oncogene that regulates gene expression program by modulating HDAC activity. PMID: 22945647
    38. SNP, rs12155400, in the histone deacetylase 9 gene (HDAC9) on chromosome 7, was associated with retinopathy in analysis of participants without hypertension PMID: 23393555
    39. Our results are consistent with the 7p21.1 association acting via promoting atherosclerosis, and consistent with alterations in HDAC9 expression mediating this increased risk. PMID: 23449258
    40. Regression models consistently identified rs2522129, rs2675231, and rs2389963 as having among the highest predictive values for explaining differences related to brain volume measures PMID: 22884548
    41. Dephosphorylation at a conserved SP motif governs cAMP sensitivity and nuclear localization of class IIa histone deacetylases HDAC4, 5 and 9 PMID: 23297420
    42. HDAC9 promotes angiogenesis and transcriptionally represses the endothelial cell miR-17-92 cluster. PMID: 23288173
    43. LBH589 and TSA may translationally regulate some HDAC encoding genes in pancreatic tumors. PMID: 22487525
    44. Data indicate a pronounced deregulation of HDAC genes HDAC9 and HDAC11 in patients with Philadelphia-negative chronic myeloproliferative neoplasms: essential thrombocythemia (ET), polycythemia vera (PV) and primary myelofibrosis (PMF). PMID: 21806350
    45. We identified a new association for large vessel stroke within HDAC9 on chromosome 7p21.1. PMID: 22306652
    46. Treated the seminoma-like cell line TCam-2 with the HDAC-inhibitor Depsipeptide. PMID: 21987446
    47. The results show that hdac9 is the third androgenic alopecia susceptibility gene PMID: 22032556
    48. HDAC9 acts as an epigenetic switch in effector T cell-mediated systemic autoimmunity. PMID: 21708950
    49. HDAC suppresses the activation of PPARgamma in the gastric carcinoma cell line SGC-7901. PMID: 19624894
    50. MITR plays a master switch role to balance osteogenic and adipogenic differentiation of MSCs through regulation of PPARgamma-2 transcriptional activity. PMID: 21247904

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  • 相关疾病：
    A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Histone deacetylase family, HD type 2 subfamily
  • 组织特异性：
    Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
  • 数据库链接：

    HGNC: 14065

    OMIM: 606543

    KEGG: hsa:9734

    STRING: 9606.ENSP00000408617

    UniGene: Hs.196054