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  4. ICAM1 Recombinant Monoclonal Antibody

ICAM1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA162789A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA162789A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA162789A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Overlay histogram showing Raji cells surface stained with CSB-RA162789A0HU (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    The first step in the preparation of recombinant ICAM1 antibody is to obtain the ICAM1 antibody gene. The heavy and light chain genes of the antibody were constructed into a plasma vector and then transfected into suspended mammalian cells transiently. After expression verification, cell supernatant was collected in expanded culture and purified recombinant ICAM1 antibody was obtained using affinity-chromatography. This recombinant ICAM1 antibody has been validated for the detection of ICAM1 protein from Human in the ELISA, IHC, FC.

    ICAM1 is an Ig-like transmembrane glycoprotein expressed in most tissues at low levels but readily increased by inflammatory cytokines. Overexpression of ICAM1 has been observed on the endothelial lumen in many pathological states. ICAM1 is involved in cell adhesion, cell signaling, and transendothelial migration of leukocytes to sites of inflammation, and antigen recognition and lymphocyte circulation and activation mediated by ICAM1 influences inflammatory conditions, nervous system development, and immune responses. Upregulation of ICAM1 has been reported in several human malignancies, including breast cancer, lung cancer, and pancreatic cancer.

  • Uniprot No.：
    P05362
  • 基因名：
  • 别名：
    Intercellular adhesion molecule 1 (ICAM-1) (Major group rhinovirus receptor) (CD antigen CD54), ICAM1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human ICAM1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3E9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    FC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.; (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.; (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.; (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
  • 基因功能参考文献：
    1. miR-335-5p could target 3'UTR of ICAM-1 and inhibit its expression, preventing invasion and metastasis of thyroid cancer cells. PMID: 30119270
    2. AGEs increase IL-6 and ICAM-1 expression via the RAGE, MAPK and NF-kappaB pathways in HGFs and may exacerbate the progression of the pathogenesis of periodontal diseases. PMID: 29193068
    3. Our meta-analyses provide no evidence of the association of ICAM-1 rs5498 with diabetic retinopathy in type 2 diabetic patients. PMID: 30419874
    4. CORM-2 inhibits P. aeruginosa-induced PGE2/IL-6/ICAM-1 expression and lung inflammatory responses by reducing the Reactive Oxygen Species generation and the inflammatory pathways. PMID: 30007888
    5. Smooth muscle cell-derived bioactive soluble factors may stimulate the ICAM-1 expression of cocultured endothelial cells, possibly leading to leukocyte migration into the subendothelial space. PMID: 29852173
    6. These results together with the s' previous study, have identified that CNOT1 provides a platform for the recruitment of TTP and CNOT7, and is involved in TTPmediated ICAM1 and IL8 mRNA decay. PMID: 29956766
    7. ICAM-1 expression is not significantly linked to metastatic disease in pancreatic ductal adenocarcinoma. PMID: 29355490
    8. In conclusion, this meta-analysis indicates that ICAM-1 gene rs5498 polymorphism decreases the risk of CAD PMID: 30290609
    9. Data suggest that serum levels of soluble ICAM1 are higher in young adults with reduced physical activity as compared to young adults who participate in optimal physical activity. This study was conducted in Bulgaria with medical and dental students aged 20 +/-2 years. PMID: 29183155
    10. TNF-alpha and IL-10 treatment can affect the expression of ICAM-1 and CD31 in human coronary artery endothelial cells. PMID: 29949812
    11. not a specific screening marker for pulmonary arterial hypertension in systemic sclerosis PMID: 29687288
    12. The ICAM-1 expression level determines the susceptibility of human endothelial cells to simulated microgravity. PMID: 29080356
    13. the combination of IL-6 -572C/G and ICAM-1 K469E polymorphisms have a synergistic effect on the onset of Sudden sensorineural hearing loss. PMID: 29695657
    14. Peripheral blood lymphocyte subsets in patients with lung cancer are different from those in healthy people, and circulating CD44+ and CD54+ lymphocytes seem to be a promising criterion to predict survival in lung cancer patients undergoing chemotherapy PMID: 29148014
    15. serum ICAM-1 levels were associated with type 2 diabetes mellitus with microalbuminuria leading to severity of diabetic kidney disease. PMID: 29310968
    16. Serum CCL2 and sICAM-1 concentrations were significantly decreased in CNS tumors in comparison with the comparative group. Among proteins tested in the serum, a higher area under the ROC curve (AUC) revealed CCL2 compared to sICAM-1 in differentiating subjects with CNS brain tumors from non-tumoral subjects. PMID: 29086194
    17. Patient-derived ATC cells overexpressed ICAM-1 and were largely eliminated by autologous ICAM-1 CAR T cells in vitro and in animal models. Our findings are the first demonstration of CAR T therapy against both a metastatic, thyroid cancer cell line and advanced ATC patient-derived tumors that exhibit dramatic therapeutic efficacy and survival benefit in animal studies PMID: 29025766
    18. Data indicate ICAM-1 as an essential receptor for both acute hemorrhagic conjunctivitis (AHC)-causing and non-AHC strains. PMID: 29284752
    19. The results of cell adhesion and western blotting assays demonstrated that arachidin-1 attenuated tumor necrosis factor (TNF)-alpha-induced monocyte/EC adhesion and intercellular adhesion molecule-1 (ICAM-1) expression PMID: 29115410
    20. anthropometric and physiological parameters do not affect the response of ICAM-1 to exercise in healthy men. PMID: 29696063
    21. 15-LOX-1 expression in colon and prostate cancer cells leads to reduced angiogenesis. These changes could be mediated by an increase in the expression of both ICAM-1 and the anti-angiogenic protein TSP-1. PMID: 28757355
    22. Single nucleotide polymorphisms in ICAM1 (rs1799969) and SERPINB2 (rs6103) genes were found to be protective against thalidomide-induced peripheral neuropathy (TiPN). In children with inflammatory bowel disease, TiPN is common but mild and generally reversible. Cumulative dose seems to be the most relevant risk factor, whereas polymorphisms in genes involved in neuronal inflammation may be protective. PMID: 28817461
    23. analysis of aberrant DNA methylation and hydroxymethylation of the ICAM1 gene promoter in the thyrocytes of Autoimmune Thyroiditis patients PMID: 28388873
    24. s demonstrate that the membrane-bound ICAM-1 isoform is necessary and sufficient to promote inflammation-dependent extracellular matrix contraction, which favors cancer cell invasion. Indeed, ICAM-1 mediates generation of acto-myosin contractility downstream of the Src kinases in stromal fibroblasts. PMID: 27901489
    25. Thrombin activated platelet releasing exosomes convey miRNA between cells. miRNA-223 regulates the expression of molecules adhesion including ICAM-1. miRNA-223 downregulated ICAM-1 mainly by impacting NF-kappaB and the MAPK pathway. PMID: 28460288
    26. Data suggest that obese children/adolescents have increased circulating biomarkers of endothelial dysfunction (here, ICAM1) and early signs of renal damage, similarly to children/adolescents with type 1 diabetes (T1D), confirming obesity to be a cardiovascular risk factor as is T1D. PMID: 27246625
    27. ICAM-1 (and IL-17) polymorphisms showed significant association with Guillian-Barre syndrome. PMID: 27595159
    28. Airway ICAM-1 expression is markedly upregulated in CAL group, which could be crucial in rhinoviral and NTHi infections. The parenchymal ICAM-1 is affected by smoking, with no further enhancement in CAL subjects. PMID: 28056984
    29. sVCAM-1 reflects xerostomia in primary Sjogren's syndrome. sICAM-1 and sE-selectin may be additional parameters of secondary Sjogren's syndrome activity. PMID: 29068581
    30. Atorvastatin strengthens Skp2 binding to FOXO1 or ICAM1, leading to ubiquitination and degradation. Skp2-dependent ubiquitination of major pathogenic molecules is the key mechanism for statin's protective effect on endothelial function in diabetes. PMID: 28802579
    31. Augmented expression of endothelial adhesion molecules ICAM1/VCAM1 is involved in the pathophysiology of patients with antiphospholipid syndrome. PMID: 29096830
    32. CD133(+) CD44(+) CD54(+) cellular subpopulation of circulating tumor cells has a prognostic value in colorectal cancer patients with liver metastasis, especially in the survival of CRC patients with liver metastasis who did not undergo surgical treatment for metastasis. PMID: 29105339
    33. Data suggest that the residue volume at phenylalanine (Phe) in alpha1-helix is critical for alpha(L)/beta(2) integrin (CD49a/CD18) activation and binding with soluble/immobilized ICAM1 (intercellular cell adhesion molecule 1). PMID: 29079572
    34. Elevated serum uric acid concentration is significantly associated with inflammation of maternal systemic vasculature as indicated by increased TNF-alpha and ICAM-1 expression in women with preeclampsia. PMID: 26511169
    35. associated with hypertension and stroke risk in women PMID: 27235695
    36. Data indicate that CDH11, ICAM1 and CLDN3 were overexpressed in tumors when compared to normal esophagus, normal gastric and non-dysplastic Barrett's. PMID: 27363029
    37. High levels of serum ICAM-1 was associated with the development of multiple organ failure . High levels of VCAM-1 was associated with both multiple organ failure and in-hospital mortality. PMID: 27701021
    38. matrix stiffness-dependent ICAM-1 clustering is an important regulator of vascular inflammation PMID: 27444067
    39. PD increased CKIP-1 and Nrf2 levels in the kidney tissues as well as improved the anti-oxidative effect and renal dysfunction of diabetic mice, which eventually reversed the up-regulation of FN and ICAM-1 PMID: 28286065
    40. PTPN22 colocalized with its substrates at the leading edge of cells migrating on surfaces coated with the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). PMID: 27703032
    41. These results suggest that SHP-2-via association with ICAM-1-mediates ICAM-1-induced Src activation and modulates VE-cadherin switching association with ICAM-1 or actin, thereby negatively regulating neutrophil adhesion to endothelial cells and enhancing their transendothelial migration. PMID: 28701303
    42. Although there was no association between sICAM-1 levels and affective temperament scores, sICAM-1 was related to the state severity of manic symptoms. PMID: 27693464
    43. It is a pro-inflammatory protein. PMID: 28390825
    44. HPMCs are capable of inhibiting growth of gastrointestinal tumors in a mechanism involving the anti-adhesive capabilities of sICAM-1 PMID: 28323210
    45. Following transepithelial migration, neutrophils adhesion to ICAM-1 resulted in activation of Akt and beta-catenin signaling, increased epithelial-cell proliferation, and wound healing. PMID: 26732677
    46. P-Selectin and ICAM-1 have roles in mediating THP-1 monocyte adhesion PMID: 28262902
    47. Dissection of the molecular mechanism revealed that the p38-Notch1 axis was the main downstream signaling pathway in CD54-mediated regulation of cancer stem cells in prostate cancers. PMID: 28042317
    48. We show that knockdown of the expression of mcircRasGEF1B reduces LPS-induced ICAM-1 expression. Additionally, we demonstrate that mcircRasGEF1B regulates the stability of mature ICAM-1 mRNAs. PMID: 27362560
    49. his study shows that two adhesion molecules, shed as soluble forms, are elevated during the acute phase of leptospirosis: E-selectin and s-ICAM1. These molecules may interfere with the process of immune cell recruitment to clear Leptospira at tissue levels. PMID: 28686648
    50. data suggest that CD2AP acts as a negative regulator of ICAM-1 clustering, which limits the formation of ICAM-1 adhesion complexes to prevent uncontrolled neutrophil adhesion and transcellular transmigration. PMID: 28484055

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, ICAM family
  • 数据库链接：

    HGNC: 5344

    OMIM: 147840

    KEGG: hsa:3383

    STRING: 9606.ENSP00000264832

    UniGene: Hs.643447