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PGAM1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA935337A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate
      All lanes: PGAM1 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 28 kDa
      Observed band size: 29 kDa
  • 其他：

产品详情

  • 产品描述：

    CUSABIO cloned the DNA sequence encoding the PGAM1 monoclonal antibody into the plasmid and then transfected into the cell line for expression. The PGAM1 monoclonal antibody resulted from the animals immunized with the synthesized peptide that was derived from human PGAM1. The product was purified through the affinity-chromatography method and then got the recombinant PGAM1 monoclonal antibody. It belongs to the rabbit IgG. This PGAM1 antibody can be used to detect the PGAM1 protein from human, mouse, and rat samples in ELISA and WB applications.

    PGAM1 is a metabolic enzyme that catalyzes the reversible conversion of 3-phosphoglycerate and 2-phosphoglycerate during the process of glycolysis. Multiple studies have demonstrated that PGAM1 is detected in a variety of tumorous tissues and is an important player in driving cancer progression and metastasis. According to the finding of Zhang et al., PGAM1 expression was linked to age, lymphatic metastasis, and tumor recurrence, and was also linked to a lower overall survival (OS) and disease-free survival (DFS).

  • Uniprot No.：
    P18669
  • 基因名：
  • 别名：
    Phosphoglycerate mutase 1 (EC 5.4.2.11) (EC 5.4.2.4) (BPG-dependent PGAM 1) (Phosphoglycerate mutase isozyme B) (PGAM-B), PGAM1, PGAMA
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide derived from human PGAM1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9A5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
  • 基因功能参考文献：
    1. High PGAM1 expression is associated with Pancreatic Ductal Adenocarcinoma Metastasis. PMID: 29386088
    2. conformation and dynamics of the C-terminal region in human phosphoglycerate mutase 1 PMID: 28748916
    3. When BPGM is knocked out, 1,3-BPG can directly phosphorylate PGAM1. In this case, PGAM1 phosphorylation and activity are decreased, but nevertheless sufficient to maintain normal glycolytic flux and cellular growth rate. PMID: 28805803
    4. study provided the first evidence revealing a non-metabolic function of PGAM1 in promoting cell migration, and gained new insights into the role of PGAM1 in cancer progression. PMID: 27991922
    5. Data show that tyrosine 26 phosphorylation enhances the binding of phosphoglycerate mutase 1 (PGAM1) to its substrates through generating electrostatic environment and structural features that are advantageous to the binding. PMID: 28076845
    6. PGAM1 in promoting homologous recombination repair in tumor cell lines. Suggest potential therapeutic opportunity for PGAM1 inhibitors in combination with PARP inhibitors in cancer treatment. PMID: 28122957
    7. PGAM1 may be associated with the grade of glioma and be involved in the biological behavior of glioma cells. PGAM1 might be a novel therapeutic target in glioma. PMID: 27572934
    8. PGAM1 correlates with spermatogenic dysfunction and affects the function of cell proliferation, apoptosis and migration. PMID: 25701843
    9. Our finding showed that PGAM1 might serve as a promising therapeutic target for UBC. PMID: 26655504
    10. PGAM1 is highly expressed in clear cell renal cell carcinoma and correlated with clinicalpathological features, which may contribute to tumor formation and progression. PMID: 26464696
    11. PGAM is acetylated at lysines 100/106/113/138 in its central region, and a member of the Sirtuin family (class III deacetylase), SIRT2, is responsible for its deacetylation. PMID: 25195573
    12. Tyrosine26 phosphorylation represents an additional acute mechanism underlying phosphoglycerate mutase 1 upregulation. PMID: 23653202
    13. Phosphoglycerate mutase 1 (PGAM1) contributes to biosynthesis regulation by controlling intracellular levels of its substrate, 3-phosphoglycerate (3-PG), and product, 2-phosphoglycerate (2-PG). PMID: 23153533
    14. PGAM1 deacetylation and activity are directly controlled by Sirt1. PMID: 22157007
    15. histidine-phosphorylated PGAM1 correlated with expression of PKM2 in cancer cell lines; decreased pyruvate kinase activity in PKM2-expressing cells allows PEP-dependent histidine phosphorylation of PGAM1 and may provide an alternate glycolytic pathway PMID: 20847263
    16. Our studies suggested that PGAM1 plays an important role in hepatocarcinogenesis PMID: 20403181
    17. Results identified phosphoglycerate mutase 1 that showed elevated levels of protein carbonyls in inferior parietal lobule (IPL) from subjects with early stage-Alzheimer's disease. PMID: 19686046
    18. Cloning, purification, crystallization and preliminary crystallographic analysis of human phosphoglycerate mutase PMID: 15388943
    19. Phosphoglycerate mutase may have a role in preventing non-spherocytic anemia [case report] PMID: 15710582
    20. We report two patients in whom phosphoglycerate mutase (PGAM) deficiency was associated with the triad of exercise-induced cramps, recurrent myoglobinuria, and tubular aggregates in the muscle biopsy. PMID: 16881065

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  • 蛋白家族：
    Phosphoglycerate mutase family, BPG-dependent PGAM subfamily
  • 组织特异性：
    Expressed in the liver and brain. Not found in the muscle.
  • 数据库链接：

    HGNC: 8888

    OMIM: 172250

    KEGG: hsa:5223

    STRING: 9606.ENSP00000359991

    UniGene: Hs.632918