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  4. PTGS1 Recombinant Monoclonal Antibody

PTGS1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA918236A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue
      All lanes: PTGS1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 69, 65, 62, 57, 72, 73 kDa
      Observed band size: 72 kDa
    • Overlay histogram showing Hela cells stained with CSB-RA918236A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    The production of the recombinant PTGS1 antibody depended on Single B Cell technology. There are 3 main steps in the production: 1, Isolation of single B cells. High-throughput methods could be used to obtain the efficient identification and desired specificity of B cells. 9, Single B cell antibody sequencing and cloning. In this step, the antibody gene sequence of PTGS1 was obtained and introduced to plasmids, which then would be transferred to mammalian cells for in vitro expression of the PTGS1 antibody. 3, Screening of antibodies. The target antibody was obtained in this step. And it has been validated in ELISA, WB, FC.

    PTGS1, also called COX-1, is a prostaglandin-producing enzyme. PTGS1 is primarily expressed in the stomach, kidney, and platelets as an intrinsic housekeeping enzyme. It primarily regulates peripheral vascular resistance, maintains renal blood flow, protects the gastric mucosa, and modulates platelet aggregation. PTGS1 is also important for the pathophysiology of inflammation, arthritic illness, and cancer. PTGS1 has been reported to be upregulated in resident inflammatory cells, which contribute to performing the acute inflammatory response and cell differentiation.

  • Uniprot No.：
    P23219
  • 基因名：
    PTGS1
  • 别名：
    Prostaglandin G/H synthase 1 (EC 1.14.99.1) (Cyclooxygenase-1) (COX-1) (Prostaglandin H2 synthase 1) (PGH synthase 1) (PGHS-1) (PHS 1) (Prostaglandin-endoperoxide synthase 1), PTGS1, COX1
  • 反应种属：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human COX1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    6B6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    FC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

引用文献

产品评价

靶点详情

  • 功能：
    Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
  • 基因功能参考文献：
    1. COX1 inhibition significantly triggered cell death. PMID: 30320345
    2. Prostaglandin D2 generation from human lung mast cells is catalysed exclusively by cyclooxygenase-1 PMID: 29225187
    3. Extracellular histones disarrange vasoactive mediators release through a COX1-COX2-eNOS interaction in human endothelial cells. PMID: 28244682
    4. Results suggested that in Chinese Han patients with stroke taking aspirin for secondary prevention, PTGS1 polymorphisms may increase the risk of poor functional outcomes and this effect may be modulated by the smoking status. PTGS1 gene-smoking interaction might in part reflect the heterogeneity in the prognosis of patients treated with aspirin. PMID: 28431615
    5. analysis of co-existing variants in both F8 and PTGS-1 genes in a three-generation pedigree of hemophilia A; the PTGS-1 variant was associated with specific functional defects in the arachidonic acid pathway and more severe hemorrhage. PMID: 27629384
    6. Data suggest expression of PTGS1 in colon is significantly correlated with expression of PTGS2, PTGES1, PTGER2, and PTGER4; this study was conducted in individuals at high risk for colon cancer treated with Mediterranean diet versus a Healthy Eating diet for prevention of colon cancer. (PG = prostaglandin; PTGS2 = PG-endoperoxide synthase 2; PTGES1 = PGE synthase protein; PTGER2 = PGE receptor 2; PTGER4 = PGE receptor 4) PMID: 27548026
    7. Epithelial Cells Induce a Cyclo-Oxygenase-1-Dependent Endogenous Reduction in Airway Smooth Muscle Contractile Phenotype PMID: 28708434
    8. Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase. PMID: 27060751
    9. Panax quinquefolium saponin attenuated HUVEC apoptosis and improved the dual antiplatelet-mediated reduction of platelet adhesion to injured HUVECs and the underlying mechanisms may be associated with PI3K/AKT and COX pathways. PMID: 27401285
    10. The interactions of COX-1of rs3842787 and cox-2 of rs20417 were associated with aspirin resistance of stroke. PMID: 27318652
    11. Results show a trend toward an association between rs1236913 SNP and worst pain scores on nonsurgical root canal therapy post-treatment day 1 PMID: 26081267
    12. We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM may play a vital role in pathological dermal angiogenesis disorders of psoriasis. PMID: 26748901
    13. a new neutrophil-activating platelet-derived lipid generated by COX-1 is presented that can activate or prime human neutrophils, suggesting a role in innate immunity and acute inflammation. PMID: 27129261
    14. Seminal COX-1 is over-expressed in infertile oligoasthenoteratozoospermic (OAT) men with varicocele (Vx) compared with fertile men with/without and infertile OAT men without Vx being associated with oxidative stress, Vx grade and Vx laterality. PMID: 25906828
    15. variable turnover rate represents the most convincing determinant of the inter-individual variability in response to aspirin for primary prevention in diabetes mellitus PMID: 26245672
    16. In Indian peptic ulcer hemorrhage patients, those with Cox-1 A842G polymorphisms tended to have less gastric ulcers among those with the A842G/C50T polymorphism. PMID: 27522738
    17. single nucleotide polymorphisms show a protective effect under a dominant model of diisocyanate-induced asthma PMID: 25721048
    18. inverse allosteric regulation likely underlies the ability of PGHS-2 to operate at low AA concentrations, when PGHS-1 is effectively latent. PMID: 26703471
    19. PON1, P2Y12 and COX1 polymorphisms were associated with poorer vascular outcomes in patients with extracranial or intracranial stents. PMID: 26870959
    20. The regulation of important oxylipin metabolic genes in peripheral blood mononuclear cells varied with the extent of change in arachidonic acid concentrations in the case of PTGS1 and ALOX12 regulation. PMID: 26672987
    21. Immunohistochemical expression of COX-1 and VEGF is associated with poor prognostic parameters in renal cell carcinoma. PMID: 26339385
    22. over-expression of COX-1 in an aberrant manner intersects multiple pro-tumorigenic pathways in high-grade serous ovarian cancer PMID: 25972361
    23. COX-2 displays an enhanced facilitatory control on tachykininergic contractile activity in diverticular disease PMID: 24758697
    24. Significant associations with NSAID-exacerbated respiratory disease were identified for: ALOX15 homozygous genotype and PTGS-1 rs5789 A/A homozygous genotype PMID: 26067486
    25. Origin of the Enigmatic Stepwise Tight-Binding Inhibition of Cyclooxygenase-1. PMID: 26562384
    26. cadmium at the highest tested concentrations modulates COX-1 and COX-2 at mRNA level in THP-1 macrophages PMID: 25645360
    27. Aspirin Half Maximal Inhibitory Concentration Value on Platelet Cyclooxygenase1 in Severe Type-2 Diabetes Mellitus is not Significantly Different from that of Healthy Individuals. PMID: 23677911
    28. Quantitative real-time PCR measurements of mRNA levels of genes PTGS1 and PTGS2 revealed no effect of the tested vanadium compound on the levels of analyzed transcripts. PMID: 25398544
    29. Data suggest that, in organization of enzymes in post-translational endoplasmic reticulum, mPGES1 (microsomal prostaglandin E synthase-1) is likely co-localized with COX2 within a distance of 14.4 A; mPGES-1 is localized much farther from COX1. PMID: 25988363
    30. Single nucleotide polymorphisms of RANK and PTGS1 show genetic associations with osteoproliferative changes in ankylosing spondylitis. PMID: 24651623
    31. Studied the impact of blood type, functional polymorphism (T-1676C) of the COX-1 gene promoter, and clinical factors on the development of peptic ulcer during cardiovascular prophylaxis with low-dose aspirin. PMID: 25243161
    32. Cigarette smoking aggravates COX-1-mediated platelet reactivity in young, otherwise healthy, smoking men. PMID: 23242413
    33. Baseline omega-3 fatty acid levels do not influence TxA(2) generation in patients with or at high risk for CVD in the absence of COX-1 activity. PMID: 24370448
    34. Data indicate that Protectin DX inhibited both cyclooxygenase-1 and -2 (COX-1 and COX-2) in neutrophils. PMID: 24254970
    35. A report of allele frequencies on COX-1 polymorphisms in pediatric patients with cardiovascular anomalies. PMID: 24535852
    36. Impacts of COX-1 gene polymorphisms on vascular outcomes in patients with ischemic stroke and treated with aspirin PMID: 24930730
    37. The association of four common polymorphisms from four candidate genes (COX-1, COX-2, ITGA2B, ITGA2) with aspirin insensitivity PMID: 24244288
    38. heterozygosity correlates with recurrent postoperative bleeding PMID: 24008976
    39. There was no association between COX-1 C50T and COX-2 G765C polymorphisms and AR in Chinese stroke patients. In addition, COX-1 C50T and COX-2 G765C polymorphisms had no effect on the platelet response to aspirin PMID: 22972377
    40. COX1 promoter polymorphisms (T-1676C and A-842G/ C50T) may not be associated with the risk of developing ulcerative colitis in a Japanese population. PMID: 18705313
    41. as COX-1 and COX-2 express and affect VEGF synthesis in HNSCC cells, we should check COX-1 expression in investigations on cancer treatment by inhibiting COX-2-induced prostaglandins. PMID: 21301321
    42. COX1 A-842G and C50T polymorphisms that contribute to resistance or augmented response to aspirin are rare in the Chinese population. PMID: 17559347
    43. SNPs in COX1 -842A>G determined thrombotic complications in myocardial infarction. PMID: 22940005
    44. COX-1 expression is widely detected in the hippocampus but decreases sharply in Niemann-Pick type C (NPC) knock-out mice compared to NPC+/+ mice. PMID: 23660496
    45. the present study did not confirm correlations between tissue mRNA levels and protein content of COX-1 and EGFR. PMID: 24171795
    46. rs2071746 in HO-1 gene, rs1330344 in COX-1 gene contribute to resistance to aspirin. PMID: 22609818
    47. In PTGS1, a functional polymorphism was associated with increased rectal cancer risk. PMID: 24022467
    48. Thromboxane A(2) generation, in the absence of platelet COX-1 activity, in patients with and without atherothrombotic myocardial infarction PMID: 23985963
    49. COX1 inhibition by aspirin is suboptimal in 15% of patients with systemic lupus erythematosus. The suboptimal response was associated with metabolic syndrome, obesity, and higher CRP concentrations. PMID: 24022862
    50. Overexpression of cyclooxygenase-1 correlates with renal cell carcinoma. PMID: 23886173

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  • 亚细胞定位：
    Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
  • 蛋白家族：
    Prostaglandin G/H synthase family
  • 数据库链接：

    HGNC: 9604

    OMIM: 176805

    KEGG: hsa:5742

    STRING: 9606.ENSP00000354612

    UniGene: Hs.201978