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  4. SELPLG Recombinant Monoclonal Antibody

SELPLG Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA567010A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA567010A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    SELPLG codes for PSGL-1, which serves as an adhesion molecule involved in immune cell trafficking and also as a regulator of many facets of immune responses by myeloid cells. PSGL-1 also regulates T cell migration during homeostasis and inflammatory settings. PSGL-1 has also been shown to regulate the localization of macrophages, dendritic cells, and B cells in the lamina propria at a steady state. Additionally, PSGL-1 mediates the adhesion of neutrophils and monocytes to P-selectin expressed on platelets that become bound to inflamed endothelium. Platelets also utilize PSGL-1 to adhere to the vasculature, suggesting that it is important for the formation of cellular complexes that function in pathogen clearance.

    The main production processes of this recombinant SELPLG antibody included immunization, B cell harvest, antibody secreting cell enrichment, single cell sequencing, antibody expression and purification. The single B cell screening platform was used for the SELPLG antibody gene screening. And this SELPLG antibody was tested in ELISA, IHC.

  • Uniprot No.：
    Q14242
  • 基因名：
  • 别名：
    P-selectin glycoprotein ligand 1 (PSGL-1) (Selectin P ligand) (CD antigen CD162), SELPLG
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CD162
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3G11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.; (Microbial infection) Acts as a receptor for enterovirus 71.
  • 基因功能参考文献：
    1. These data indicate that cardiac surgery influences the expression of CD162, CD166 and CD195 and that the intensity of the immune system response, displayed as the change in the CD162, CD166, CD195 expression, varies, depending on the surgical technique used. PMID: 27625334
    2. PSGL1 mediated leukocyte rolling and the inflammatory response in general.PSGL1 is a ligand for Siglec-5 and interacts with Siglec-5 ectodomain. PMID: 27892504
    3. The Interaction of Selectins and PSGL-1 as a Key Component in Thrombus Formation and Cancer Progression. PMID: 28680883
    4. Studies indicate that P-selectin glycoprotein ligand-1 (PSGL-1) PSGL-1 can negatively regulate T cell function. PMID: 28262471
    5. Results suggest that PSGL-1 may play an oncogenic role in the development of intestinal tumors, and this is likely mediated through activation of NFkB signaling by MIP-1g. PMID: 28108624
    6. c-Myc regulates P-selectin glycoprotein ligand-1 expression in monocytes during HIV-1 infection. PMID: 28663244
    7. The percentage of CXCR3(+) CD4(+) TEM cells negatively correlated with the severity of the cutaneous disease in psoriasis patients. Importantly CLA(+) CD4(+) TCM cells expressing CCR6(+) or CCR4(+)CXCR3(+) negatively correlated with psoriasis severity suggesting recruitment to the skin compartment. PMID: 28392462
    8. Platelet-leukocyte aggregations increased in acute ischemic stroke patients rapidly within 3h. The I allele of PSGL-1 M62I was associated with risk of developing acute ischemic stroke, especially large artery atherosclerosis stroke and small artery occlusion stroke. Small artery occlusion stroke patients with the II genotype of PSGL-1 M62I have the higher level of platelet-neutrophil aggregates. PMID: 27423570
    9. The significant presence of CLA+ T cells and E-selectin expressions in the OLPG suggests their involvement in the etiopathogenesis of OLP; however, only a weak correlation between CLA+ T cells and E-selectin was observed. PMID: 26332489
    10. PSGL-1 is a novel receptor for S. pneumoniae that contributes to protection against invasive pneumococcal disease. PMID: 26975045
    11. Report influence of SELPLG variation on leukocyte-platelet interactions in cardiovascular disease. PMID: 25428141
    12. CD162 staining and the staining degree, with the other standard immunohistochemical stains, were shown to be beneficial in the diagnosis of multiple myeloma disease. PMID: 25445472
    13. Staphylococcal SElX and SSL6 proteins bind cell surface receptors PSGL-1 and CD47, respectively. PMID: 24840181
    14. SCARB2 and PSGL-1 in human gastrointestinal tract, lung, and brain tissues correlated with the distribution of pathological changes seen in EV71 infection. PMID: 24997419
    15. Host membrane protein PSGL-1, CD43, and CD44 association with assembling HIV-1 Gag is driven by polybasic sequences present in the cytoplasmic tails of the membrane proteins and in Gag. PMID: 25320329
    16. Up-regulation of PSGL-1 on CD14++CD16+ monocytes may play a crucial role in plaque rupture and thrombus formation. PMID: 24583418
    17. lymphocyte trafficking into the CNS under VLA-4 blockade can occur by using the alternative adhesion molecules, PSGL-1 and MCAM, the latter representing an exclusive pathway for TH17 cells to migrate over the blood-brain barrier PMID: 25135296
    18. ligation of PSGL-1 on HL-60 cells activates beta2 integrin, for which lipid raft integrity and Syk activation are responsible PMID: 24312591
    19. Specific phosphorylations on 130 and 153 tyrosine residues of p85-bound Rho-GDP dissociation inhibitor-2 are pivotal for P-selectin glycoprotein ligand-1-induced beta1 integrin-mediated lymphocyte adhesion to vascular cell adhesion molecule-1. PMID: 24055812
    20. PSGL-1 has Janus-faced functions, being both adhesive and anti-adhesive. PMID: 23192782
    21. Phosphoinositide 3-kinase (PI3K) is required for PSGL-1-induced beta1 integrin clustering which ultimately regulates beta1 integrin-mediated Jurkat cell adhesion to fibronectin. PMID: 24122451
    22. In conclusion, s report PSGL-1 as a novel target for pneumococcal ZmpC and show that ZmpC inhibits neutrophil extravasation during pneumococcal pneumonia. PMID: 23601501
    23. Data suggest that PSGL1 on leukocytes is binding site for VP1 (a capsid protein) in Enterovirus 71; conserved lysine residues on virus surface are responsible for interaction with sulfated tyrosine residues in PSGL1 N-terminus. PMID: 23935488
    24. Expression of CD11b and CD162 on monocytes has a temperature-dependent regulation, with decreased expression during hypothermia. PMID: 23812215
    25. Psoriasis patients' tonsils have a higher frequency of skin-homing CLA [cutaneous lymphocyte-associated antigen] positive T cells, correlating significantly with their frequency of blood CLA+ T cells. PMID: 23750651
    26. A receptor-dominated endocytosis of enterovirus 71 infection was observed: PSGL-1 initiates caveola-dependent endocytosis and hSCARB2 activates clathrin-dependent endocytosis. PMID: 23760234
    27. The aim of the study was to investigate the degree of platelet activation in NSCLC and the roles of PSGL-1 in the activation of platelets. PMID: 22535539
    28. These results indicated that SCARB2 is capable of viral binding, viral internalization, and viral uncoating and that the low infection efficiency of L-PSGL1 cells is due to the inability of PSGL1 to induce viral uncoating. PMID: 23302872
    29. effect of mutation on binding affinity to P- and L-selectin PMID: 22947939
    30. There is significant association between PSGL-1 Met62Ile polymorphism and advanced carotid atherosclerosis with stenosis in female patients from the Serbian population. PMID: 22307784
    31. E- and P-selectins play a role in spontaneous metastasis formation both into bone marrow and lungs. PMID: 22366582
    32. PSGL-1 EBS plays a critical role in recruiting leukocytes on selectins and in activating the MAPK pathway, whereas it is dispensable to phosphorylate Syk and to lead to alpha(L)beta(2)-dependent leukocyte slow rolling. PMID: 22311979
    33. Data show the contribution of P-selectin glycoprotein ligand-1 (PSGL-1) to the regulation of growth, dissemination, and drug resistance in multiple myeloma (MM) cells in the context of the bone marrow microenvironment. PMID: 22096244
    34. ADAM8 is both associated with PSGL-1 through the ezrin-radixin-moesin actin-binding proteins and able to cause the proteolytic cleavage of this adhesion receptor. PMID: 22229154
    35. PSGL-1 microparticles show a characteristic course during alloSCT and their possible association with relapse/progress requires further evaluation of the PSGL-1/P-selectin interaction in leukemias and lymphomas PMID: 21864485
    36. Coxsackievirus A24 variant bound better to mouse L929 cells overexpressing human P-selectin glycoprotein ligand-1 (PSGL-1) than to mock-transfected cells, suggesting that PSGL-1 is a candidate receptor for the virus. PMID: 21880775
    37. Healthy volunteers were genotyped and the adhesion of neutrophils expressing the PSGL-1 isoforms A/A, A/B and B/B were studied under static and physiologic flow conditions. PMID: 21144558
    38. IL-6 induces neutrophilia and reduces CD162 expression on neutrophils in inflammation PMID: 21292497
    39. Tyrosine sulfation of the amino terminus of PSGL-1 is associated with enterovirus 71 infection. PMID: 21079683
    40. The results suggest that an adaptive mutation, along with a PSGL-1-binding phenotype, may facilitate efficient PSGL-1-dependent replication of enterovirus 71 strains in L-PSGL-1 cells. PMID: 20943886
    41. Results suggested that Syk plays a key role in signal transduction downstream of PSGL-1 in endothelial progenitor cells. PMID: 20705591
    42. cutaneous lymphocyte-associated antigen expression might be an indicator of poor prognosis in non-nasal NK-cell lymphomas with nodal presentation PMID: 19628338
    43. There is a strong expression of both SELPLG and coronin-1C in the majority of primary effusion lymphomas, irrespective of their gene dosage. SELPLG is critical for cell migration and chemotaxis, while CORO1C regulates actin-dependent processes. PMID: 20690162
    44. Deficiency of Psgl-1 is associated with reduced IL-1 receptor-mediated adhesive properties of the endothelium. PMID: 20558823
    45. Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters PMID: 11916843
    46. G-CSF down regulates PSGL-1 expression on the surface of neutrophils in humans PMID: 11961238
    47. Expression of cutaneous lymphocyte-associated antigen by CD8(+) T cells specific for herpes simplex virus type 2(cutaneous lymphocyte-associated antigen) PMID: 12189248
    48. Binding of two anti-human (KPL1 and PL1) and two anti-mouse (4RA10 and 2PH1) PSGL-1 mAbs to synthetic peptides of N-terminus of human and mouse PSGL-1 was found to be independent of tyrosine sulfation. PMID: 12223514
    49. The epitope recognized by the M-DC8 MAb is 6-sulfo LacNAc, a novel carbohydrate modification of the P selectin glycoprotein ligand 1 (PSGL-1) PMID: 12354382
    50. increased PSGL-1 expression on granulocytes from allergic-asthmatic subjects resulted in increased leukocyte recruitment on P-selectin under flow conditions PMID: 12377939

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed on neutrophils, monocytes and most lymphocytes.
  • 数据库链接：

    HGNC: 10722

    OMIM: 600738

    KEGG: hsa:6404

    STRING: 9606.ENSP00000228463

    UniGene: Hs.591014