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  4. SERPINH1 Recombinant Monoclonal Antibody

SERPINH1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA699016A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA699016A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    SERPINH1 codes for HSP47, a chaperone located in the endoplasmic reticulum that is involved in collagen biosynthesis. Aberrant expression of SERPINH1 has been observed in several human cancers. It has been demonstrated that high expression of SERPINH1 apparently lowered the overall survival (OS),disease-specific survival, and progression-free interval in some certain cancer types. Upregulation of the SERPINH1 gene is linked to poor prognosis in breast cancer, stomach adenocarcinoma, and esophageal carcinoma.

    The main steps in the production of this SERPINH1 recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The SERPINH1 antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, IHC.

  • Uniprot No.：
    P50454
  • 基因名：
    SERPINH1
  • 别名：
    Serpin H1 (47 kDa heat shock protein) (Arsenic-transactivated protein 3) (AsTP3) (Cell proliferation-inducing gene 14 protein) (Collagen-binding protein) (Colligin) (Rheumatoid arthritis-related antigen RA-A47), SERPINH1, CBP1 CBP2 HSP47 SERPINH2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Hsp47
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    10B2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 基因功能参考文献：
    1. Our present data suggest that HSP47 is an important prognostic factor and an attractive therapeutic target in laryngeal squamous cell carcinoma (LSCC)due to its influence on the biological behaviour of LSCC cells PMID: 28849239
    2. an endoplasmic reticulum complex of resident chaperones that includes HSP47, FKBP65, and BiP regulating the activity of LH2. PMID: 28177155
    3. These observations indicate that this system is appropriate for detecting the interaction between HSP47 and collagen, and could be applied to high-throughput screening for drugs capable of suppressing and/or curing fibrosis. PMID: 29438711
    4. miR-29b can reduce collagen biosynthesis during skin wound healing likely via post-transcriptional inhibition of HSP47 expression. PMID: 27477081
    5. HSP47 expression in patients with colorectal cancer and the number of HSP47-positive spindle cells in the tumor stroma were significantly higher compared with those in adjacent normal colonic mucosa, and the number of the latter cells increased with tumor progression. PMID: 27925182
    6. The essential parts of the Golgi stress response from the perspective of the organelle autoregulation. The pathways of the mammalian Golgi stress response have been identified, specifically the HSP47 pathway. PMID: 28179603
    7. Overexpression of LOXL2 and SERPINH1 was observed in clinical specimens of lung cancer and fibrotic lesions. Downregulation of miR-29a caused overexpression of LOXL2 and SERPINH1 in lung cancer and IPF, suggesting that these genes are involved in the pathogenesis of these two diseases. PMID: 27488440
    8. The changes in the SERPINH1 and SERPINF1 genes in patients with osteogenesis imperfect were synonymous polymorphisms or missense changes located in non-coding regions. PMID: 27706701
    9. Overexpression of HSP47 is associated with poor prognosis in patients with esophageal squamous cell carcinoma and this is consistent with the function of HSP47 in terms of increased cell proliferation and colony formation. PMID: 25953518
    10. A novel homozygous variant in SERPINH1 associated with a severe, lethal presentation of osteogenesis imperfecta with hydranencephaly. PMID: 27677223
    11. The present study demonstrates that HSP47 promotes glioma angiogenesis and highlights the importance of HSP47 as an attractive therapeutic target of GBM. PMID: 25758142
    12. Although the chemical chaperone 4-PBA partially restores the solubility of the Hsp47 OI mutants, collagen-binding activity of Hsp47 was not improved. PMID: 26692483
    13. Data show that the expression of heat shock protein 47 (HSP47) was increased in the peripheral blood mononuclear cells and plasma from scleroderma patients. PMID: 26091621
    14. Mutations in the HSP47 and FKBP65 produce a moderately severe form of Osteogenesis imperfect. PMID: 25510505
    15. In patients with schistosomiasis japonica, TGF-beta1 participates not only in the inflammatory process, but also in the fibrotic process in which Hsp47 and CTGF probably play a key role. PMID: 25111595
    16. Hsp47 expression promotes cancer progression in part by enhancing deposition of extracellular matrix proteins. PMID: 25744716
    17. IL-17A-induced HSP47 expression is involved in collagen I expression in intestinal subepithelial myofibroblasts, which might contribute to intestinal fibrosis in Crohn's disease. PMID: 24534724
    18. silencing of the HSP47 gene significantly inhibited cell migration and invasion in cancer cells and the expression of HSP47 was upregulated in cancer tissues and cervical intraepithelial neoplasia, as demonstrated by immunostaining. PMID: 24141696
    19. miR-29b down-regulates HSP47 and LOX expression. PMID: 24650661
    20. HSP47 is a novel glioma-associated antigen PMID: 24623841
    21. TRAIL induced HSF1 inactivation leads to the suppression of Hsp47-dependent collagen production in activated human hepatic stellate cells. PMID: 23587601
    22. Correlative Hsp47 expression in fibroblasts with bFGF in inflammatory cells may contribute to stromal fibrosis and obstruction in colorectal carcinoma PMID: 23265436
    23. NMR and mutational identification of the collagen-binding site of the chaperone Hsp47 PMID: 23049894
    24. Hsp47 may be related to the TGF-beta1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts. PMID: 22967132
    25. Hsp47 recognizes the triple-helix form of procollagen in vitro and in vivo. PMID: 22235129
    26. Overexpression of HSP47 decreased the secretion of heterotrimers containing the mutant collagen alpha5(IV) chain. PMID: 21187648
    27. HSP47 and fascin expression may play role in the pathogenesis of invasive ductal carcinoma of the breast and prostatic adenocarcinoma because their expression is significantly higher than their normal counterpart. PMID: 20701077
    28. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis PMID: 19067716
    29. down-regulated KLF4, CHGA, GPX3, SST and LIPF, together with up-regulated SERPINH1, THY1 and INHBA is an 8-gene signature for gastric cancer PMID: 20043075
    30. Increased heat shock protein 47 expression is associated with esophageal squamous cell carcinoma. PMID: 20112500
    31. The enhancement of HSP47 expression by TGF-beta and IL-1 beta has been confirmed in embryonic lung fibroblasts. PMID: 11994473
    32. induced in cicatricial pemphigoid: possible role(s) in dermal fibrosis PMID: 12061838
    33. Results indicate a novel means by which type I collagen production is regulated by the endoplasmic reticulum constituent, Hsp47. PMID: 12163502
    34. Modulates the production of the endostatin precursor collagen XVIII in head and neck carcinomas PMID: 12174873
    35. levels of HSP47 protein and autoantibodies to HSP47 in the sera of patients with rheumatic autoimmune diseases PMID: 12659832
    36. gene expression profiling in epidermolysis bullosa acquisita PMID: 12824005
    37. high levels of expression of Hsp47 and adult and oncofetal fibronectin in Dupuytren's contracture suggests that cell-mediated alterations in the extracellular environment may play an important role in the disease process PMID: 15047128
    38. HSP47 has a role in aging and photoaging in human fibroblasts PMID: 15247019
    39. The surface-exposed RA-A47 may induce autoantibodies and inflammatory reactions in autoimmune disease situations such as rheumatoid arthritis. PMID: 15389525
    40. HSP47 is constitutively expressed in human hepatic stellate cells and may be a target for antifibrotic therapy. PMID: 15806139
    41. Our results suggest the existence of different fibrotic pathways among these groups involved in the expression of HSP47 and type I procollagen. PMID: 15955241
    42. results suggest nitric oxide has dual effects on collagen synthesis by fibroblasts: the direct stimulation of collagen synthesis due to the up-regulation of procollagen alphaI(1) mRNA, and an indirect effect through the increase of HSP47 mRNA expression PMID: 16171977
    43. analysis of the client recognition mechanism of HSP47 PMID: 16326708
    44. analysis of recognition of the collagen triple helix by chaperone HSP47 PMID: 16484215
    45. A functional SNP in the promoter of the SERPINH1 gene increases risk of preterm premature rupture of membranes in African Americans. PMID: 16938879
    46. The anti-fibrotic effect of pirfenidone may be mediated through direct inhibition of collagen type I expression and inhibition of HSP47 expression in lung fibroblasts. PMID: 18093617
    47. HSP47-positive fibroblasts were main constituent cell of dermatofibroma. PMID: 18095990
    48. These studies define a new haplotype in the SERPINH1 gene that modifies risk of an adverse obstetrical outcome. PMID: 18205191
    49. During keratin preparation from cultured human tumor cell lines, Hsps might be associated with keratin expression in tumor cells PMID: 18293509
    50. Hsp47 was exposed on surface of GPVI-activated platelets; inhibition of Hsp47 abolished platelet aggregation in response to collagen, but partially reduced aggregation in response to other agonists; propose Hsp47 may play a role in hemostasis & thrombosis PMID: 19341245

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  • 相关疾病：
    Osteogenesis imperfecta 10 (OI10)
  • 亚细胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    Serpin family
  • 数据库链接：

    HGNC: 1546

    OMIM: 600943

    KEGG: hsa:871

    STRING: 9606.ENSP00000350894

    UniGene: Hs.596449