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  4. SUZ12 Recombinant Monoclonal Antibody

SUZ12 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA159561A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate
      All lanes: SUZ12 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 84 kDa
      Observed band size: 90 kDa
    • IHC image of CSB-RA159561A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunoprecipitating SUZ12 in K562 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-RA159561A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-RA159561A0HU(2µg)+ K562 whole cell lysate(500µg)
      Lane 3: K562 whole cell lysate (10µg)
  • 其他：

产品详情

  • 产品描述：

    SUZ12 is a core component of the PRC2 complex that plays an essential role in PRC2-mediated gene silencing by producing trimethylation on H3K27me3. SUZ12 is rarely expressed in normal tissues, but amplification and overexpression of SUZ12 have been detected in several solid malignancies including ovarian, colorectal, and breast cancer. Its abnormal overexpression is commonly related to tumor aggressive behaviors like enhanced proliferation, invasion, and metastasis, as well as advanced clinicopathological characteristics, and reduced survival. SUZ12 has also been shown to play a role in the cell cycle and X chromosome inactivation.

    The main steps in the production of this SUZ12 recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The SUZ12 antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, WB, IHC, IP.

  • Uniprot No.：
    Q15022
  • 基因名：
    SUZ12
  • 别名：
    Polycomb protein SUZ12 (Chromatin precipitated E2F target 9 protein) (ChET 9 protein) (Joined to JAZF1 protein) (Suppressor of zeste 12 protein homolog), SUZ12, CHET9 JJAZ1 KIAA0160
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human SUZ12
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1B10
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IP 1:200-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • 基因功能参考文献：
    1. Functional assays to reveal the targets of n-miR-G665 showed that polycomb protein Suz12 is regulated by n-miR-G665, which in turn regulates the expression of n-miR-G665 through feedback loop mechanism. PMID: 29379063
    2. Data suggest the polycomb repressive complex 2 subunits EZH2, SUZ12, and EED protein axis as promising therapeutic target for treating sarcoma. PMID: 29415665
    3. High SUZ12 expression is associated with invasion and metastasis of tumor budding cells in tongue squamous cell carcinoma. PMID: 27582550
    4. we analyzed eight probands with clinically suspected Weaver syndrome by whole-exome sequencing and identified three mutations: a 25.4-kb deletion partially involving EZH2 and CUL1 ,a missense mutation , and a missense mutation in SUZ12 inherited from her father .In vitro functional analyses demonstrated that the identified EED and SUZ12 missense mutations cause decreased decreased trimethylation of lysine 27 of H3 PMID: 28229514
    5. PRE-PIK3C2B interacts directly with YY1 in vitro and recruits PRC2 complex in vivo. The localization of PcG proteins including YY1 to PRE-PIK3C2B in HEK cells is decreased on knock-down of either YY1 or SUZ12. PMID: 23805300
    6. EZH1, SUZ12 and UXT work synergistically to regulate pathway activation in the nucleus. PMID: 27127229
    7. Mutation in the SUZ12 gene is associated with malignant peripheral nerve sheath tumors. PMID: 28124441
    8. Overexpression of miR-105 inhibited cell proliferation, tumorigenesis, migration, invasion, and drug sensitivity, whereas overexpression of suppressor of Zeste 12 homolog antagonized the tumor-suppressive functions of miR-105. PMID: 28618952
    9. Mutations of SUZ12 and EED are reported to have tumor suppressive functions. (Review) PMID: 27000413
    10. The difference in the SUZ12 and CBX8 genes expression were significantly divergent between tumors and their marginal tissues. PMID: 26837964
    11. Activation of EZH2 and SUZ12 Regulated by E2F1 Predicts the Disease Progression and Aggressive Characteristics of Bladder Cancer PMID: 26268246
    12. Data show that RAS protein activation was the initiating oncogenic event, with polycomb repressive complex 2 (PRC2) subunits impairment being a secondary event. PMID: 26457648
    13. SUZ12 is a key molecule in the regulation of monoallelic expression of IGF2. PMID: 26407907
    14. Overexpression of Suz12 was associated with lung adenocarcinoma. PMID: 25279705
    15. found that expression of PcG proteins and H3K27me3 showed prognostic value in our study cohort PMID: 25243792
    16. this study described that miR-489 expression level was significantly reduced in lung cancer samples (n = 115) and cell lines. Abnormal expression of miR-489 regulated the cells invasion by binding SUZ12 3'-UTR in vitro. PMID: 25833694
    17. downregulation of SUZ12 and ZNF198 leads to epigenetic reprogramming of infected hepatocytes. Because both Plk1 and HOTAIR are elevated in many human cancers, we propose that their combined effects are involved in epigenetic reprogramming PMID: 25855382
    18. Conclude that the expression of SUZ12 is increased along with the degree of infiltration and metastasis of gastric carcinoma. PMID: 26176059
    19. Data show that overall enhancer of zeste 2 (EZH2), embryonic ectoderm development (EED) and suppressor of zeste 12 homolog (SUZ12) expression in the colorectal cancer (CRC) tissues was significantly increased than in the non-cancerous tissue. PMID: 25326896
    20. Somatic mutations of SUZ12 have a central role in malignant transformation of peripheral nerve sheath tumors. PMID: 25305755
    21. Polycomb repressive complex 2 is recurrently inactivated through EED or SUZ12 loss in malignant peripheral nerve sheath tumors. PMID: 25240281
    22. reduction in the total SUZ12 level was not a sufficient cause of the resolution of the epigenetic bivalency of beta-cell transcription factors in embryonic stem cells. PMID: 24845830
    23. malat1 promotes bladder cancer metastasis by associating with suz12. PMID: 24449823
    24. Data shows EZH2 or SUZ12 depletion leads to HP1 loss from chromatin and degradation. PMID: 25047840
    25. the polycomb group gene SUZ12 functions as tumour suppressor in peripheral nerve sheath tumors, high-grade gliomas and melanomas by cooperating with mutations in NF1 PMID: 25119042
    26. provide evidences suggesting that SUZ12 is an oncogene in non-small cell lung cancer PMID: 24633887
    27. postulate that this interparalog structural difference, together with low allelic recombination rates, could have caused a reduction in NAHGC between SUZ12 and SUZ12P during human evolution PMID: 24385046
    28. SUZ12 depletion mediated by RNA interference (RNAi) led to a reduction of gastric cell numbers and arrested the cell cycle at G1/S point. PMID: 23735840
    29. Polycomb Repressive Complex 2 overexpression is associated with small cell lung cancer. PMID: 23714854
    30. SUZ12 inactivation results in apoptosis in human colon cancer stem cells. PMID: 23588203
    31. SUZ12 promotes the proliferation of human EOC cells by inhibiting apoptosis and HRK is a novel SUZ12 target gene whose upregulation contributes to apoptosis induced by SUZ12 knockdown. PMID: 22964433
    32. studies suggest a tumor suppressor role for PRC2 in human leukemia and suggest a hitherto unrecognized dynamic interplay between oncogenic NOTCH1 and PRC2 function PMID: 22237151
    33. Genetic defects in PRC2 components other than EZH2 are not common in myeloid malignancies. PMID: 22308284
    34. We propose Plk1 activity down-regulates ZNF198 and SUZ12, thereby enhancing both HBV replication and pX-mediated oncogenic transformation. PMID: 21480320
    35. Results support the hypothesis that the abnormal expression of SUZ12 accounts for some of the unexplained features of mantle cell lymphoma, such as abnormal DNA repair and increased resistance to apoptosis. PMID: 20558579
    36. histone modification including PRC2-mediated repressive histone marker H3K27me3 and active histone marker acH4 may involve in CD11b transcription during HL-60 leukemia cells reprogramming to terminal differentiation[polycomb repressive complex 2 ] PMID: 19578722
    37. Loss of miR-200 during CSC formation increases Suz12 expression, Suz12 binding, H3-K27 trimethylation, and Polycomb-mediated repression of the E-cadherin gene. PMID: 20832727
    38. SUZ12 blocks cell differentiation, as SUZ12 knockdown releases differentiation programs in a chronic myeloid blastic phase transformed cell line. PMID: 19847889
    39. Endometrial stromal tumors(ESTs) are genetically heterogeneous, with prevalence of JAZF1-JJAZ1 fusion highest among ESTs of classic histology. Diagnostic utility of JAZF1-JJAZ1 fusion transcript assay in ESTs may be limited to classic histologic subset. PMID: 15043312
    40. JJAZ1 gene recombination may have a role in mosaicism in neurofibromatosis type 1 PMID: 15257518
    41. Data show that SUZ12 is distributed across large portions of over two hundred genes encoding key developmental regulators. PMID: 16630818
    42. The JAZF1-JJAZ1 fusion is a frequent, although nonuniform, feature of endometrial stromal neoplasia. PMID: 17197920
    43. SUZ12 regulates H3-K9 methylation in an EZH2-independent fashion. Knockdown of SUZ12 in human cells caused a reduction in H3K27me3 and H3K9me3, and altered the distribution of HP1 alpha. PMID: 17406994
    44. The JAFZ1-JJAZ1 fusion gene is present in the endometrial stromal and smooth muscle components of endometrial stromal tumors. PMID: 17667554
    45. SUZ12, a component of the polycomb repressor complex 2, is recruited to the beta-globin gene. PMID: 17970752
    46. Both benign and malignant forms of endometrial stromal tumor have the JAZF1-JJAZ1 fusion, but only the malignant form also exhibits exclusion of the unrearranged JJAZ1 allele. PMID: 18077430
    47. the two PRC2 components SUZ12 and EZH2 are sumoylated in vitro and in vivo PMID: 18628979
    48. YY1 physically interacts with SUZ12 and can act as a mediator to recruit the polycomb group proteins and DNA methyltransferases to participate in the CEBPD gene silencing process PMID: 18753137
    49. study shows normal endometrial stromal cells contain a specific chimeric RNA joining 5' exons of JAZF1 gene on chromosome 7p15 to 3' exons of JJAZ1/SUZ12 gene on chromosome 17q11; this RNA is translated into JAZF1-JJAZ1 with anti-apoptotic activity PMID: 18772439
    50. The JAZF1-JJAZ1 gene fusion was not identified in any Uterine tumor resembling ovarian sex cord tumors PMID: 19542872

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  • 相关疾病：
    A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    VEFS (VRN2-EMF2-FIS2-SU(Z)12) family
  • 组织特异性：
    Overexpressed in breast and colon cancer.
  • 数据库链接：

    HGNC: 17101

    OMIM: 606245

    KEGG: hsa:23512

    STRING: 9606.ENSP00000316578

    UniGene: Hs.462732