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TBP Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA821481A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, HL60 whole cell lysate
      All lanes: TBP antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 38, 36 kDa
      Observed band size: 36 kDa
  • 其他：

产品详情

  • 产品描述：

    The first step in the preparation of recombinant TBP antibody is to obtain the TBP antibody gene. The heavy and light chain genes of the antibody were constructed into a plasma vector and then transfected into suspended mammalian cells transiently. After expression verification, cell supernatant was collected in expanded culture and purified recombinant TBP antibody was obtained using affinity-chromatography. This recombinant TBP antibody has been validated for the detection of TBP protein from Human in the ELISA, WB.

    TBP is an important component of the transcription initiation machinery in eukaryotes. TBP is crucial for all archaeal-eukaryotic transcription initiation complexes, and it's the only factor that allows all three eukaryotic and archaeal transcription systems to attain full states of initiation. It attaches to the central promoter and, through interactions with other factors, acts as a nexus for gene regulation. TBP levels have an impact on the transcription of genes involved in the cell cycle. TBP is also involved in the transmission of active gene memory to daughter cells during mitosis.

  • Uniprot No.：
    P20226
  • 基因名：
    TBP
  • 别名：
    TATA-box-binding protein (TATA sequence-binding protein) (TATA-binding factor) (TATA-box factor) (Transcription initiation factor TFIID TBP subunit), TBP, GTF2D1 TF2D TFIID
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human TBP
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3C7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
  • 基因功能参考文献：
    1. these studies support the idea that increases in TBP expression contribute to enhanced VEGFA transcription early in colorectal cancer development to drive tumorigenesis. PMID: 28415573
    2. The genetic animal models of SCA17 clearly indicate that the phenotypes are dependent on the expression levels of mutant TBP and the length of CAG/glutamine repeat. PMID: 27859490
    3. we observed that carriers of either ATXN7 or TBP alleles with relatively large CAG repeat sizes in both alleles had a substantially increased risk of lifetime depression. PMID: 28585930
    4. Data indicate the crystal structure of a Brf2-TBP-Bdp1 complex bound to a DNA promoter. PMID: 28743884
    5. Study could not determine a definitive cutoff value for the pathologic CAG repeat number of SCA17. PMID: 26267067
    6. TBP attenuates Msx1-mediated glycoprotein hormone alpha transcriptional repression. PMID: 26505791
    7. this analysis of the 2.2-kb doubly spliced RNA (2.2DS-RNA) -mediated suppression of viral RNA expression showed that 2.2DS-RNA inhibited transcription via binding to the TATA-binding protein and stress granule proteins. PMID: 26339052
    8. Our results provide first evidence that Taspase1 processing affects TFIIA regulation of TFIID and suggest that Taspase1 processing of TFIIA is required to establish INR-selective core promoter activity in the presence of NC2. PMID: 25996597
    9. TBP (and GATA2 and Sp1) have key roles in inflammation and ischemia-like conditions through MAOA regulation. PMID: 25810277
    10. Deactivation of TBP contributes to SCA17 pathogenesis. PMID: 25104854
    11. HOXA2 acts as a suppressor or TBP-antagonist to inhibit MMP-9 expression; while methylation-mediated inactivation of HOXA2 in NPC derepresses MMP-9 production and increases invasion of NPC cells. PMID: 24243817
    12. The data reveal synergistic effects of H3K4me3, H3K14ac and a TATA box sequence on TFIID binding in vitro. Stoichiometry analyses of affinity purified human TFIID identified the presence of a stable dimeric core. PMID: 24039962
    13. The UL4 protein directly interacted with the host TBP and the carboxy-terminal domain of RNA polymerase II in infected cells. PMID: 24418534
    14. Findings indicate that using TATA-binding protein (TBP) alone or in combination with hydroxymethylbilane synthase (HMBS) as endogenous controls could be a reliable method for normalizing qRT-PCR data in hepatoma cell lines treated with TNF-alpha. PMID: 23811755
    15. Ethanol increases Pol III transcription through a response element which is composed of the overlapping Elk1 and AP-1 binding sites of the TBP promoter. The binding sites may play a role in ethanol-induced deregulation of Pol III genes in liver tumors. PMID: 23454483
    16. conclude that the oncoprotein HBXIP as a co-activator of TF II D transactivates Lin28B promoter via directly binding to TBP to upregulate the expression of Lin28B in promotion of proliferation of breast cancer cells PMID: 23494474
    17. This study showed that five families with SCA17 represents a significant portion of ataxia cases in the population of the Czech Republic. PMID: 22872568
    18. This study created a first transgenic rat model for SCA17 that carries a full human cDNA fragment of the TBP gene with 64 CAA/CAG repeats (TBPQ64). PMID: 23699518
    19. effects of promoter TATA-box polymorphisms associated with human diseases on interactions between the TATA boxes and TATA-binding protein PMID: 23424617
    20. These studies suggest a model in which the distinct conformations of TFIID may serve as targets through which regulatory factors recruit TFIID to specific types of core promoters. PMID: 23332750
    21. TATA binding protein (TBP) interactions with oligonucleotides PMID: 23062343
    22. CONCLUSIONS: The current case highlights the diagnostic challenge of neurodegenerative disorders and the need for a thorough clinical and paraclinical examination of patients. PMID: 22889412
    23. The autosomal dominantly inherited progressive neurodegenerative disorder is caused by an expanded CAA/CAG repeat in the TATA-box binding protein PMID: 17853080
    24. In Korean population, the mutation frequencies of SCA2 and SCA17 were similar. whereas SCA17 was a more significant cause of parkinsonism. PMID: 21334959
    25. Expression of the transgenic TBP in Purkinje cells is sufficient to produce cell degeneration and an ataxia phenotype, and constitutes a good model for analysis of neurodegeneration in spinocerebellar ataxia type 17. PMID: 21554323
    26. This study demonstrated mutant TATA box-binding protein at the endogenous level affects neuronal function, with important implications for the pathogenesis and treatment of polyglutamine diseases. PMID: 21705419
    27. The data suggest that ICP4 interacts with components of TFIID and Mediator in the context of viral infection. PMID: 21450820
    28. TBP mutations may be a very rare cause of Parkinson's disease in mainland China PMID: 20864379
    29. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression. PMID: 21106530
    30. Brain weight correlated with the 'raw' (i.e. non-normalized) data for two mRNAs, beta2-microglobulin and TATA-binding protein, measured in cerebellum and hippocampus, respectively PMID: 20831744
    31. Regulation of TBP by miR-146a may contribute to Huntington disease pathogenesis. PMID: 20451497
    32. The DNA bend angle (theta) depends on the TATA sequence, with theta for coreTBP and human TBP being greater than that for yeast TBP. PMID: 19199812
    33. In lymphoblastoid cells, HSPA5, HSPA8 and HSPB1 expression levels in cells with expanded SCA17 were significantly lower than that of the control cells. PMID: 20004653
    34. TATA-binding protein-associated factor sharply decrease the rate at which Pol II, TFIIB, and TFIIF assemble on promoter-bound TFIID-TFIIA. PMID: 20083121
    35. constitutive association of TBP with mitotic chromosomes PMID: 11809839
    36. TBP can bind the TATA box through a regulated two-step mechanism, involving a transition from unbent complex to bent complex PMID: 11893333
    37. Developmental specificity of recruitment of TBP to the TATA box of the human gamma-globin gene PMID: 11960008
    38. Human papillomavirus-16 E7 protein inhibits the DNA interaction of the TATA binding transcription factor. PMID: 11968006
    39. Data show that TATA-binding protein labelling was relatively more abundant than huntingtin labelling in the brains of Huntington disease patients, and increased with the grade of the disease. PMID: 12531510
    40. Data show that human TATA box binding protein (TBP) can use a shared surface to interact with two different transcription factor IIB (TFIIB) family members to initiate transcription by different RNA polymerases. PMID: 12535529
    41. spatial positioning of the DNA-bound activation domain is important for efficient activation, possibly by maximizing its interactions with the transcriptional machinery including the TBP-TFIIA-TFIIB-promoter quaternary complex PMID: 12538582
    42. TBP expression is elevated in human colon carcinomas relative to normal colon epithelium. Both Ras-dependent and Ras-independent mechanisms mediate increases in TBP expression in colon carcinoma cell lines. PMID: 12697807
    43. Sequence-dependent solution structure and motions of TBP complexes were studied. PMID: 12767124
    44. simultaneously binds and bends promoter DNA without a slow isomerization step or TFIIB requirement PMID: 12791683
    45. Our data provides biochemical evidence that Mediator functions by facilitating activator-mediated recruitment of pol II and also promoter recognition by TBP, both of which can occur in the absence of TBP-associated factors in TFIID PMID: 12917344
    46. p300 plays a role in formation of the TBP-TFIIA-containing basal transcription complex, TAC. PMID: 12941701
    47. Results present the X-ray structures of human and yeast TATA box-binding protein /transcription factor IIA/DNA complexes at 2.1A and 1.9A resolution, respectively. PMID: 12972251
    48. in addition to its role in regulating TBP binding to a TATA box, the TBP surface is unexpectedly involved in TBP association with all three TFIIB family members PMID: 14585974
    49. abnormal expansions of an allele in SCA8 and SCA17 genes were detected in patients with both Parkinson's disease and spinocerebellar ataxia PMID: 14756671
    50. Analysis of Spinocerebellar ataxia type 17 (SCA17) locus in a group of ataxic patients excluded on other known SCAs. PMID: 14763955

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  • 相关疾病：
    Spinocerebellar ataxia 17 (SCA17)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TBP family
  • 组织特异性：
    Widely expressed, with levels highest in the testis and ovary.
  • 数据库链接：

    HGNC: 11588

    OMIM: 600075

    KEGG: hsa:6908

    STRING: 9606.ENSP00000230354

    UniGene: Hs.590872