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TINF2 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA997969A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: RAW264.7 whole cell lysate, HL-60 whole cell lysate, Mouse Herat whole cell lysate, Mouse Brain whole cell lysate
      All lanes: Tin2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 51, 40, 16 kDa
      Observed band size: 51 kDa
  • 其他：

产品详情

  • 产品描述：

    TINF2 is the encoding gene for TIN2, which acts as an adaptor crucial for the recruitment, formation, and stabilization of the shelterin complex. It mediates the localization of three key DNA interacting proteins (TRF1, TRF2, and POT1) at telomeres. It is essential for shelterin assembly at the telomere and is an important regulator of telomere length. Mutations in TINF2 cause short-telomere syndromes. Deletion of TIN2 is embryonically lethal, and conditional knockout of TIN2 in mouse embryo fibroblasts (MEFs) leads to growth arrest. Upregulation of TIN2 has been commonly observed in many human malignancies.

    The recombinant TINF2 antibody production commenced with the obtaining of genes encoding antibody against TINF2. Antibody genes were obtained by sequencing and screening DNA reversely transcribed from RNA that was extracted from the B cells isolated from immunized animals. These genes were cloned into plasma vectors and subsequently transfected into a mammalian cell line for production. The product is the recombinant TINF2 antibody. It underwent purification using Affinity-chromatography from the cell culture medium. This recombinant TINF2 antibody has been validated to detect the TINF2 protein from Human, Mouse, Rat in the ELISA, WB.

  • Uniprot No.：
    Q9BSI4
  • 基因名：
    TINF2
  • 别名：
    TERF1-interacting nuclear factor 2 (TRF1-interacting nuclear protein 2), TINF2, TIN2
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide derived from human Tin2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    5G11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Plays a role in shelterin complex assembly. Isoform 1 may have additional role in tethering telomeres to the nuclear matrix.
  • 基因功能参考文献：
    1. The TIN2 plays an important role in maintaining the stable shelterin complex required for proper telomere end protection. PMID: 29160297
    2. We report ophthalmic findings in twins with Revesz syndrome due to a previously unreported mutation in TINF2 and propose that phenotypic and molecular overlaps between DKC spectrum disorders and pediatric retinal vasculopathies may reflect a shared pathophysiologic basis. PMID: 28095086
    3. demonstrate here the presence of a novel spliced isoform of TIN2 in chronic lymphocytic leukemia (CLL), related to deletion of exon 2 in the TIN2 gene PMID: 28575699
    4. data supports a mechanism whereby telomerase deficiency and subsequent shortened telomeres initiate a DNA damage response and create a pro-oxidant environment, especially in Dyskeratosis Congenita cells carrying TINF2 mutations PMID: 26859482
    5. telomerase elongates telomeres at a reduced frequency in TIN2-R282H heterozygous cells; this recruitment defect is further corroborated by examining the effect of this mutation on telomerase-telomere co-localization. PMID: 26230315
    6. Our findings identify TINF2 as a mutant telomere gene in familial pulmonary fibrosis and suggest that infertility may precede the presentation of pulmonary fibrosis in a small subset of adults with telomere syndromes PMID: 25539146
    7. A potential mitotic regulation of TIN2 by phosphorylation, is reported. PMID: 23977114
    8. results suggest a link between telomeric proteins (TIN2)and metabolic control, providing an additional mechanism by which telomeric proteins regulate cancer and aging PMID: 22885005
    9. Results suggests that the disruption of TIN2-TRF1 interaction may contribute to the severe dyskeratosis congenita phenotype observed in the context of the TIN2 truncation mutation. PMID: 21477109
    10. The positive correlation between telomere length in dyskeratosis congenital and percent of LINE-1 methylation was restricted to TINF2 mutations. PMID: 21981348
    11. Frameshift or nonsense mutations in TIN2 gene having very short telomeres are found in patients with dyskeratosis congenita. PMID: 21199492
    12. Siah2 acts as an E3 ligase to directly ubiquitylate TIN2 in vitro. PMID: 22064479
    13. These results demonstrate the important roles that Sp1 and NF-kappaB play in regulating the expression of the human telomere-binding protein TIN2. PMID: 21731707
    14. The presence of dysfunctional telomeres in chronic lymphocytic leukemia did not correlate with telomere shortening or chromatin marks deregulation but with a down-regulation of 2 shelterin genes: ACD and TINF2. PMID: 21355086
    15. TIN2 mutations in DC may compromise the telomere recruitment of telomerase, leading to telomere shortening and the associated pathogenesis. PMID: 21536674
    16. exon 6 of TINF2 natural mutations in 2/142 Japanese patients with acquired acquired bone marrow failure syndromes PMID: 20560964
    17. TINF2 mutation of shelterin complex is associated with dyskeratosis congenita. PMID: 20979174
    18. The expression of TINF2 may work to reduce the telomere length in precancerous lesions and offer new insight into the mechanism of carcinogenesis in gastric cancer. PMID: 20127252
    19. TIN2-anchored TPP1 plays a major role in the recruitment of telomerase to telomeres in human cells. PMID: 20404094
    20. Co-localises with the interstitial TTAGGG repeats in interstitial telomeres. PMID: 11938440
    21. Down-regulation of TRF1, TRF2 and TIN2 genes is important to maintain telomeric DNA for gastric cancers. PMID: 12530079
    22. TIN2 alters the conformation of TRF1, which favours a tertiary telomeric structure that hinders telomerase from gaining access to telomeres PMID: 12835755
    23. Partial knockdown of TIN2 by small hairpin RNA in a telomerase-positive cell line resulted in telomere elongation, which is typical of reduced TRF1 function. PMID: 15133513
    24. TIN2 mutants defective in binding of TRF1 or TRF2 induce a DNA damage response and destabilize TRF1 and TRF2 at telomeres in human cells. PMID: 15292264
    25. TIN2 binds TRF1 and TRF2 simultaneously and stabilizes the TRF2 complex on telomeres PMID: 15316005
    26. May be involved in multistep hepatocarcinogenesis by playing crucial role in telomere shortening. PMID: 15632001
    27. a novel extra-telomeric organization of TIN2 is associated with the control of cell proliferation; TIN2 is an important regulator of mammary epithelial differentiation PMID: 15741234
    28. coordinated interactions among TPP1, TIN2, TRF1, and TRF2 may ensure robust assembly of the telosome, telomere targeting of its subunits, and, ultimately, regulated telomere maintenance PMID: 16880378
    29. TINF2, a component of the shelterin telomere protection complex, is mutated in dyskeratosis congenita. PMID: 18252230
    30. Results suggest that distinct TIN2 complexes exist and that TIN2-15C-sensitive subcomplexes are particularly important for cell survival in the absence of functional p53. PMID: 18443218
    31. In a large series, TINF2 mutations account for approximately 11% of all patients with dyskeratosis congenita. PMID: 18669893
    32. conclude that a significant proportion of patients who underwent unrelated donor hematopoietic stem cell transplantation for severe aplastic anemia harbored mutations in TINF2 and may have had occult dyskeratosis congenita PMID: 19090550
    33. Our results suggest a dual role for TIN2 in mediating the function of the shelterin complex and tethering telomeres to the nuclear matrix. PMID: 19229133

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  • 相关疾病：
    Dyskeratosis congenita, autosomal dominant, 3 (DKCA3); Dyskeratosis congenita, autosomal dominant, 5 (DKCA5)
  • 亚细胞定位：
    Nucleus. Chromosome, telomere. Note=Associated with telomeres.; [Isoform 1]: Nucleus matrix.
  • 组织特异性：
    Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 数据库链接：

    HGNC: 11824

    OMIM: 268130

    KEGG: hsa:26277

    STRING: 9606.ENSP00000267415

    UniGene: Hs.496191