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  4. TPT1 Recombinant Monoclonal Antibody

TPT1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA214107A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue
      All lanes: TPT1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 20, 16 kDa
      Observed band size: 25 kDa
    • IHC image of CSB-RA214107A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of SY5Y Cells with CSB-RA214107A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
    • Immunoprecipitating TCTP in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-RA214107A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-RA214107A0HU(2µg)+ Hela whole cell lysate(500µg)
      Lane 3: Hela whole cell lysate (10µg)
  • 其他：

产品详情

  • 产品描述：

    TPT1 is a highly conserved protein abundantly present in all eukaryotic organisms, and it plays a role in nearly all of the biological processes that drive growth, stress response, and survival. TPT1 can be secreted to enhance basophil histamine production thus provoking allergic responses and inflammation. TPT1 is typically increased in malignancies, resulting in apoptosis inhibition, metastasis promotion, and anticancer therapy resistance. The pro-survival roles of TPT1 are manifested in multiple relevant aspects of tumorigenesis and malignant transformation, including genome stability, cell cycle, mechanisms of apoptosis, mitotic/meiotic progression, PI3K/AKT/TOR signaling, epithelial-mesenchymal Transformation (EMT), pluripotent stem cell induction and autophagy.

    The recombinant TPT1 antibody expression is induced in mammalian cells transfected with a recombinant plasma vector. The recombinant plasma vector was constructed by inserting the gene coding for the antibody against TPT1 into the plasma. The recombinant TPT1 antibody was purified from the cell culture medium using Affinity-chromatography. It can react with samples containing TPT1 protein from Human, Mouse and has been validated for use in the ELISA, WB, IHC, IF, IP.

  • Uniprot No.：
    P13693
  • 基因名：
    TPT1
  • 别名：
    Translationally-controlled tumor protein (TCTP) (Fortilin) (Histamine-releasing factor) (HRF) (p23), TPT1
  • 反应种属：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human TCTP
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    10A9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
    IP 1:200-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in calcium binding and microtubule stabilization.
  • 基因功能参考文献：
    1. Study findings suggested that TCTP promotes colorectal cancer metastasis through regulating the behaviors of HMGB1 and the downstream activation of the NF-kappaB signaling pathway. PMID: 30066846
    2. The biological effects of Na,K-ATPase and TCTP interaction are reviewed. PMID: 29867020
    3. The cells knocked down for TCTP show diminished invasiveness and migration under TGF-beta1 treatment. TGF-beta1 dependent TCTP expression is required for epithelial to mesenchymal transition in cells. PMID: 28958626
    4. that TPT1 knockdown altered the BECN1 interactome, a representative MTOR-independent pathway, to stimulate autophagosome formation PMID: 28409693
    5. Transfection with TCTP-Lipid modified antisense oligonucleotides led to rapid and prolonged internalization via macropinocytosis, TCTP downregulation and significant decreased cell viability in in castration-resistant prostate cancer cells. PMID: 28472637
    6. extracellular TCTP has a role as a promoter of colorectal cancer progression and liver metastases via Cdc42/JNK/MMP9 activation PMID: 27367023
    7. The structure of human HRF (hHRF) was solved in 2 new crystal forms, one at the highest resolution (1.4A) yet reported. The high resolution hHRF structure reveals a disulphide-linked dimer, in which the 2 molecules are closely associated and provides a model for the role of HRF in mast cell activation. PMID: 29216544
    8. analyzed the interactome of TCTP in HeLa cells by coimmunoprecipitation (IP) and mass spectrometry (MS). Results not only reveal a large number of TCTP-associated proteins that possess pleiotropic functions, but also provide novel insights into the molecular mechanisms of TCTP in tumorigenesis PMID: 27607350
    9. Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels. PMID: 28550308
    10. These findings provide novel data on the role and regulation of TCTP in skeletal muscle in vivo. PMID: 27813490
    11. TCTP may thus contribute to the development of anti-cancer drug resistance. PMID: 28143584
    12. The findings suggest that the synergistic actions of Histamine Releasing Factor and Histamine Releasing Factor-reactive IgE may play important roles in the pathogenesis of Chronic Urticaria. PMID: 28219065
    13. Downregulation of the expression of TCTP inhibits the migration and invasion of glioma cells. PMID: 26328748
    14. These data may suggest an involvement of translationally controlled tumor protein in normal physiology and carcinogenesis. PMID: 26425551
    15. High HRF expression is associated with non-Hodgkin lymphomas. PMID: 25363345
    16. Results demonstrate that TCTP mRNA translation is regulated by signalling through the PI3-K/Akt/mTORC1 pathway in neoplasms. PMID: 25936523
    17. High phospho-TCTP expression levels are associated with aggressive breast cancer. PMID: 25779659
    18. TCTP binds to hemin and functions as a "buffer protein", controlling cellular homeostasis. PMID: 25396429
    19. analysis of how TCTP-PTD penetrates A549 human lung cancer cell membranes and promotes protein internalization PMID: 25423047
    20. Data indicate that Tanshinone IIA (Tan-IIA)T upregulated the protein expression of CHOP and Bax, but downregulated the protein expression of BiP, TCTP, Mcl-1 and Bcl-xL. PMID: 25270224
    21. Targeting Hsp27 interaction with TCTP, a partner protein identified in a genetic screen, increases therapy sensitivity, and could decrease or abolish toxicity to normal cells. PMID: 25277244
    22. TCTP is functionally implicated in the progression of NF1-associated tumors. PMID: 25092287
    23. osteoclastogenic activity of translationally-controlled tumor protein (TCTP) with reciprocal repression of p21 PMID: 25263704
    24. Interaction of translationally controlled tumor protein with Apaf-1 is involved in the development of chemoresistance in HeLa cells. PMID: 24606760
    25. High TCTP expression is associated with glioma. PMID: 24311645
    26. Plasmodium falciparum TCTP showed a lower B cell proliferative effect than human TCTP. PMID: 24465583
    27. Cofilin-1 expression positively correlates with the tumor grade, and p23 expression correlates with the endoplasmic reticulum positive status. PMID: 24106833
    28. TCTP is a novel mediator of endothelial prosurvival and growth in patients with pulmonary arterial hypertension. PMID: 24657995
    29. observations indicate that TCTP enhances Pim-3 stability to simultaneously promote and prevent cell-cycle progression and apoptosis, respectively PMID: 24165482
    30. TCTP may have a role in prostate cancer development PMID: 23894469
    31. Data indicate that translationally controlled tumor protein (TCTP) expression in glioma was an independent prognostic factor, which might be a potential diagnostic and therapeutic target of glioma. PMID: 23749504
    32. TCTP binds to the beta domain of VHL through competition with HIF1alpha, which promotes VHL degradation by the ubiquitin-proteasome system and HIF1alpha stability. PMID: 23387829
    33. human miR-27b regulates the expression of the TCTP tumor protein, and circulating miR-27b may be useful as a biomarker for oral cancer research. PMID: 22902387
    34. Demonstrate the presence of TCTP in human cornea, and suggest a potential role in the pathogenesis of herpes virus keratitis. PMID: 22853445
    35. Results imply that TCTP might be a mediator of PRL-3-promoted proliferation, migration and invasion of human colon cancer cells. PMID: 22340241
    36. Data suggest that TCTP may play a critical role in maintaining genomic integrity in response to DNA-damaging agents. PMID: 22451927
    37. CHD1L/TCTP/Cdc25C/Cdk1 molecular pathway causes the malignant transformation of hepatocytes with the phenotypes of accelerated mitotic progression and the production of aneuploidy PMID: 21953552
    38. The TCTP is a survival factor that protects cancer cells from oxidative stress-induced cell-death. PMID: 21801721
    39. These results identify TCTP-bearing nanovesicles as a novel component of the paracrine apoptotic program of potential importance in vascular repair. PMID: 20966960
    40. The Chfr-TCTP interaction was stable throughout the cell cycle, but it could be diminished by the complete depolymerization of the microtubules. PMID: 18504434
    41. Knockdown of TCTP inhibited proliferation, migration, and invasion activities of LoVo cells in vitro and in vivo. PMID: 19621893
    42. Data suggest a kind of feedback regulation between TCTP and VDR to regulate a variety of (Ca(2+) dependent) cellular effects and in this way further underscore the physiological relevance of the observed physical interaction beteen TCTP and VDR. PMID: 19815065
    43. The mRNA of the translationally controlled tumor protein P23/TCTP is a highly structured RNA, which activates the dsRNA-dependent protein kinase PKR (TCTP PROTEIN) PMID: 11991642
    44. Physical and functional interaction between myeloid cell leukemia 1 protein (MCL1) and this protein PMID: 12149273
    45. Human TCTP was identified as a putative Plk-interacting clone by a two-hybrid screen. Plk phosphorylates TCTP on two serine residues in vitro and cofractionates with the majority of kinase activity toward TCTP in mitotic cell lysates. PMID: 12167714
    46. genetic regulation in tumor reversion PMID: 12399545
    47. results do not support the hypothesis that IgE(mn+) induces a late allergic reaction (LAR), but do not exclude the alternative hypothesis that HRFs are released during a LAR and contribute to asthma severity PMID: 12487227
    48. Patients with Multiple drug allergy syndrome and more than 1/3 with hypersensitivity to a single antibacterial drug showed the presence of circulating histamine-releasing factors. Such factors might play a role in drug-induced adverse reactions. PMID: 12876410
    49. HRF p23 can stimulate nonimmune epithelium such as bronchial epithelium PMID: 12948934
    50. Human recombinant histamine-releasing factor can partially inhibit the secretion of IL-2 and IL-13 protein in a dose-dependent manner from both purified peripheral blood T cells and Jurkat T cells. PMID: 14500674

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TCTP family
  • 组织特异性：
    Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC
  • 数据库链接：

    HGNC: 12022

    OMIM: 600763

    KEGG: hsa:7178

    STRING: 9606.ENSP00000431872

    UniGene: Hs.374596