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  4. Recombinant Apis mellifera Apamin

Recombinant Apis mellifera Apamin

  • 货号：
    CSB-YP360687DNK
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP360687DNK
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP360687DNK-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP360687DNK
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP360687DNK
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Apamin; Apamine
  • 种属：
    Apis mellifera (Honeybee)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    28-45
  • 氨基酸序列
    CNC KAPETALCAR RCQQH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Neurotoxin that blocks the small-conductance calcium-activated potassium (SK) channels KCa2.1/KCNN1/SK1, KCa2.2/KCNN2/SK2 and KCa2.3/KCNN3/SK3. Also irreversibly blocks the voltage-gated potassium channel Kv1.3/KCNA3 (IC(50)=13 nM). Potently blocks human, rat and mouse KCa2.2/KCNN2/SK2 channels (IC(50)=27-140 pM), and moderately blocks human and rat KCa2.3/KCNN3/SK3 channels (IC(50)=0.6-4 nM), and human (IC(50)=0.7-12 nM) and mouse (IC(50)=28 nM) KCa2.1/KCNN1/SK1 channels. However, other reports found that it did not block the human KCa2.1/KCNN1/SK1 channels. Also able to inhibit neuromuscular transmission by a mechanism independent of the blockade of SK channels, which may involve the activation of inhibitory muscarinic M2 receptors on motor nerve terminals.
  • 基因功能参考文献：
    1. These results demonstrate the potential of apamin for the prevention of EMT progression induced by TGF-beta1 in vitro and CCl4-injected in vivo. PMID: 24878534
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed by the venom gland.
  • 数据库链接：

    KEGG: ame:406135

    UniGene: Ame.1216