Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Apis mellifera Phospholipase A2

Recombinant Apis mellifera Phospholipase A2

  • 货号：
    CSB-YP018091DNK
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018091DNK-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018091DNK
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018091DNK
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Phospholipase A2; bvPLA2; EC 3.1.1.4; Allergen Api m I; Phosphatidylcholine 2-acylhydrolase; allergen Api m 1
  • 种属：
    Apis mellifera (Honeybee)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    34-167
  • 氨基酸序列
    IIYPGTL WCGHGNKSSG PNELGRFKHT DACCRTHDMC PDVMSAGESK HGLTNTASHT RLSCDCDDKF YDCLKNSADT ISSYFVGKMY FNLIDTKCYK LEHPVTGCGE RTEGRCLHYT VDKSKPKVYQ WFDLRKY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • 基因功能参考文献：
    1. PLA(2)s were located on the membrane surface rather than penetrating the membrane bilayer and that the deeply inserted mode is not a favorable condition for the hydrolysis of phospholipids in zwitterionic phospholipid membranes. PMID: 21557547
    2. contributions of the individual disulfide bonds to conformational stability PMID: 20884319
    3. successive introduction of sequence modifications outside the human and murine T cell epitope regions led to a progressive loss of specific IgE and IgG reactivity PMID: 15459335
    4. Studies with bee venom (bv) mutant PLA2 show that electrostatic interactions between lysine and arginine residues and anionic phosphatidylserine contribute minimally to overall interfacial binding energetics of bvPLA2. PMID: 15491136
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Phospholipase A2 family, Group III subfamily
  • 组织特异性：
    Expressed by the venom gland.
  • 数据库链接：

    KEGG: ame:406141

    STRING: 7460.GB13351-PA

    UniGene: Ame.2