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  4. Recombinant Aquifex aeolicus DNA mismatch repair protein mutL (mutL)

Recombinant Aquifex aeolicus DNA mismatch repair protein mutL (mutL)

  • 中文名称：
    藻类含水层mutL重组蛋白
  • 货号：
    CSB-YP529062DNV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    藻类含水层mutL重组蛋白
  • 货号：
    CSB-EP529062DNV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    藻类含水层mutL重组蛋白
  • 货号：
    CSB-EP529062DNV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    藻类含水层mutL重组蛋白
  • 货号：
    CSB-BP529062DNV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    藻类含水层mutL重组蛋白
  • 货号：
    CSB-MP529062DNV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    mutL
  • Uniprot No.：
  • 别名：
    mutL; aq_1578; DNA mismatch repair protein MutL
  • 种属：
    Aquifex aeolicus (strain VF5)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-425
  • 氨基酸序列
    MFVKLLPPEV RKVIAAGEVI ESPVDVVKEL VENSLDAKAT KVEVEIVKGG KRLIRVKDNG TGIHPEDVEK VVLQGATSKI ETEKDLMNIS TYGFRGEALY SISSVSKFKL RSRFFQEKEG KEIEVEAGNI LGTRRVGMPV GTEVEVRDLF FNLPVRRKFL KKEDTERRKV LELIKEYALT NPEVEFTLFS EGRETLKLKK SSLKERVEEV FQTKTEELYA EREGITLRAF VSRNQRQGKY YVFINKRPIQ NKNLKEFLRK VFGYKTLVVL YAELPPFMVD FNVHPKKKEV NILKERKFLE LVRELAGKEK PIVDIPLSQP VKTYKPTYEI LGQMDETFIL VKDSEYLYFV DQHLLEERIN YEKLKDENLA CRISVKAGQK LSEEKIRELI KTWRNLENPH VCPHGRPIYY KIPLREIYEK VGRNY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.
  • 基因功能参考文献：
    1. The overall structure of the ATPase domain of MutL was comprised of 2 subdomains: the N-terminal ATP-binding subdomain and the C-terminal alpha-beta sandwich subdomain. Site-directed mutagenesis experiment identified DNA-interacting 8 basic AAs distributed across both the 2 subdomains and formed a DNA-binding cleft. The 2 separate DNA-binding sites in the full-length A. aeolicus MutL simultaneously bind a dsDNA molecule. PMID: 28668638
    2. The N-terminal domain (NTD) of MutL is monomeric and exhibits a relatively strong DNA-binding activity; it is also clear that NTD promotes the endonuclease activity of carboxyl-terminal domain (CTD). PMID: 20961292
    3. A. aeolicus MutL is a human PMS2 homologue that stimulates a PcrA/UvrD/Rep subfamily helicase PMID: 20129926
    4. Adenosine triphosphate stimulates Aquifex aeolicus MutL endonuclease activity PMID: 19777055
  • 蛋白家族：
    DNA mismatch repair MutL/HexB family
  • 数据库链接：

    KEGG: aae:aq_1578

    STRING: 224324.aq_1578