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  4. Recombinant Arabidopsis thaliana 12-oxophytodienoate reductase 3 (OPR3)

Recombinant Arabidopsis thaliana 12-oxophytodienoate reductase 3 (OPR3)

  • 中文名称：
    拟南芥OPR3重组蛋白
  • 货号：
    CSB-YP884329DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥OPR3重组蛋白
  • 货号：
    CSB-EP884329DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥OPR3重组蛋白
  • 货号：
    CSB-EP884329DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥OPR3重组蛋白
  • 货号：
    CSB-BP884329DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥OPR3重组蛋白
  • 货号：
    CSB-MP884329DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    OPR3
  • Uniprot No.：
  • 别名：
    OPR3; DDE1; At2g06050; F5K7.19; 12-oxophytodienoate reductase 3; EC 1.3.1.42; 12-oxophytodienoate-10,11-reductase 3; AtOPR3; OPDA-reductase 3; Protein DELAYED DEHISCENCE 1) [Cleaved into: 12-oxophytodienoate reductase 3; N-terminally processed]
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-391
  • 氨基酸序列
    MTAAQGNSNE TLFSSYKMGR FDLSHRVVLA PMTRCRALNG VPNAALAEYY AQRTTPGGFL ISEGTMVSPG SAGFPHVPGI YSDEQVEAWK QVVEAVHAKG GFIFCQLWHV GRASHAVYQP NGGSPISSTN KPISENRWRV LLPDGSHVKY PKPRALEASE IPRVVEDYCL SALNAIRAGF DGIEIHGAHG YLIDQFLKDG INDRTDQYGG SIANRCRFLK QVVEGVVSAI GASKVGVRVS PAIDHLDATD SDPLSLGLAV VGMLNKLQGV NGSKLAYLHV TQPRYHAYGQ TESGRQGSDE EEAKLMKSLR MAYNGTFMSS GGFNKELGMQ AVQQGDADLV SYGRLFIANP DLVSRFKIDG ELNKYNRKTF YTQDPVVGYT DYPFLAPFSR L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specifically cleaves olefinic bonds in cyclic enones. Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules. Required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program. In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively. Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT).
  • 基因功能参考文献：
    1. The s found that OPR3 specifically inhibited primary root growth under phosphate deficiency via suppressing root tip growth at the transcriptional level, revealing an important novel function of OPR3 in regulating primary root response to the nutrient stress. PMID: 27101793
    2. In structures of substrate-free and 8-iso-PGA1 bound AtOPR3, the two monomers observed in the asymmetric unit of AtOPR3 crystals can be superimposed with the corresponding protomers of the LeOPR3 dimer, suggesting a similar dimeric structure for AtOPR3. PMID: 21915915
    3. Over-expression of OPR3 does not strongly induce BPEp accumulation, but exogenous application of jasmonate restores BPEp expression in the opr3 mutant. PMID: 19765234
    4. OPR3 was found to be induced by touch, wounding, methyl jasmonate, NaCl and CaCl(2) while ethylene and darkness had no effect. PMID: 16533544
    5. brassinosteroid-responsive gene PMID: 16807820

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  • 亚细胞定位：
    Peroxisome.
  • 蛋白家族：
    NADH:flavin oxidoreductase/NADH oxidase family
  • 组织特异性：
    Expressed in green seedling, leaves, flowers (anthers, pistil, petal and stamen), and to a lower extent in roots and siliques. Specifically expressed in filament during anther dehiscence initiation.
  • 数据库链接：

    KEGG: ath:AT2G06050

    STRING: 3702.AT2G06050.1

    UniGene: At.1135