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  4. Recombinant Arabidopsis thaliana Auxin-responsive protein IAA3 (IAA3)

Recombinant Arabidopsis thaliana Auxin-responsive protein IAA3 (IAA3)

  • 中文名称：
    拟南芥IAA3重组蛋白
  • 货号：
    CSB-YP653623DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥IAA3重组蛋白
  • 货号：
    CSB-EP653623DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥IAA3重组蛋白
  • 货号：
    CSB-EP653623DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥IAA3重组蛋白
  • 货号：
    CSB-BP653623DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥IAA3重组蛋白
  • 货号：
    CSB-MP653623DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    IAA3
  • Uniprot No.：
  • 别名：
    IAA3; SHY2; At1g04240; F19P19.32; Auxin-responsive protein IAA3; Indoleacetic acid-induced protein 3; Short hypocotyl; Suppressor of HY2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-189
  • 氨基酸序列
    MDEFVNLKET ELRLGLPGTD NVCEAKERVS CCNNNNKRVL STDTEKEIES SSRKTETSPP RKAQIVGWPP VRSYRKNNIQ SKKNESEHEG QGIYVKVSMD GAPYLRKIDL SCYKGYSELL KALEVMFKFS VGEYFERDGY KGSDFVPTYE DKDGDWMLIG DVPWEMFICT CKRLRIMKGS EAKGLGCGV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Plays a central role in auxin regulation of root growth, in gravitropism, and in lateral root formation. Regulated by an auxin-induced protein turnover. Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. When activated by cytokinin, restricts the expression of the PIN genes to the vascular transition zone. Induction of SHY2 in the vascular transition zone restricts BRX expression to down-regulate PIN3 and thus limit meristem growth, but proper SHY2 expression requires BRX. Involved in meristem growth and in determining its size. May participate in strigolactone signaling to regulate meristem size and lateral root formation.
  • 基因功能参考文献：
    1. SHY2 is a pivotal negative regulator of root growth, which has been demonstrated to efficiently repress transcription of major auxin transport facilitators of the PIN-FORMED (PIN) gene family, thereby restricting polar auxin transport to the root tip and in consequence auxin-driven primary root growth. Taken together, our results disclose the central low-energy activated SnRK1-C/S1-bZIP signalling module as gateway to PMID: 28158182
    2. DA1-related protein 2 (DAR2) acts downstream of cytokinin and SHY2 but upstream of PLT1/2 to affect root meristem size. PMID: 23518585
    3. Strigolactone signaling involves SHY2 protein activity in roots. PMID: 23425316
    4. The shy2 mutations strongly elevate endogenous indole-3-acetic acid level in the roots. PMID: 22527388
    5. Data suggest a model in which the regulatory interactions favor BRX expression in the early proximal meristem and SHY2 prevails because of supplementary cytokinin induction in the later distal meristem. PMID: 21149702

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Aux/IAA family
  • 组织特异性：
    Highly expressed in stems and flowers. Expressed in hypocotyls, cotyledons and leaves, but barely detected in roots. Expressed in root tips. In the root meristem, specifically detected at the vascular tissue transition zone.
  • 数据库链接：

    KEGG: ath:AT1G04240

    STRING: 3702.AT1G04240.1

    UniGene: At.20801