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  4. Recombinant Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1), partial

Recombinant Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1(BAK1),partial,Yeast
  • 货号：
    CSB-YP850098DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1(BAK1),partial,Yeast
  • 货号：
    CSB-EP850098DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1(BAK1),partial,Yeast
  • 货号：
    CSB-EP850098DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1(BAK1),partial,Yeast
  • 货号：
    CSB-BP850098DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1(BAK1),partial,Yeast
  • 货号：
    CSB-MP850098DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    BAK1; ELG; SERK3; At4g33430; F17M5.190BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1; AtBAK1; BRI1-associated receptor kinase 1; EC 2.7.10.1; EC 2.7.11.1; Protein ELONGATED; Somatic embryogenesis receptor kinase 3; AtSERK3; Somatic embryogenesis receptor-like kinase 3
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Controls the expression of genes associated with innate immunity in the absence of pathogens or elicitors. Involved in brassinosteroid (BR) signal transduction. Phosphorylates BRI1. May be involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-BAK1 heterodimers. Interaction with MSBP1 stimulates the endocytosis of BAK1 and suppresses brassinosteroid signaling. Acts in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) via its interactions with FLS2 and EFR, and the phosphorylation of BIK1. Involved in programmed cell death (PCD) control. Positively regulates the BR-dependent plant growth pathway and negatively regulates the BR-independent cell-death pathway. Phosphorylates BIR2 and thus promotes interaction with BIR2. This interaction prevents interaction with FLS2 in the absence of pathogen-associated molecular patterns (PAMP). Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity. Required for PSK promotion of seedling growth and protoplast expansion. CNGC17 and AHAs form a functional cation-translocating unit that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes. Probably required during small peptide (e.g. RGF1) signaling.
  • 基因功能参考文献：
    1. BRI1 and BAK1 have roles interacting with G proteins and regulating sugar-responsive growth and development in Arabidopsis PMID: 29670153
    2. the BAK1 C-terminal tail is differentially required for its functions in development and immunity. PMID: 29227029
    3. BIR3 interacts with BAK1 and inhibits ligand binding receptors to prevent BAK1 receptor complex formation. PMID: 28842532
    4. The role of BAK1 in defense against root-knot nematode and pattern-triggered immunity PMID: 26892116
    5. an allosteric mechanism for inhibition of BAK1 by C408 S-glutathionylation PMID: 29211989
    6. Study identifies a link between plant perception of biotic threats by BAK1, cellular calcium entry mediated by GLRs, and intracellular calcium release by TPC1 during a biologically relevant interaction. PMID: 28559475
    7. The Arabidopsis Malectin-Like/LRR-RLK IOS1 Is Critical for BAK1-Dependent and BAK1-Independent Pattern-Triggered Immunity PMID: 27317676
    8. These findings show that BAK1 contains an additional AtRALF1 binding site, indicating that this protein may be part of a AtRALF1-containing complex as a co-receptor, and it is required for the negative regulation of cell expansion PMID: 29028796
    9. the existence of partial redundancy between SERK3 and SERK1 as well as the promoting or repressive activity of a single coreceptor in multiple simultaneously active pathways, is reported. PMID: 27803191
    10. The found that in an otherwise "active" BAK1 the alphaC helix is highly disordered, a hallmark of deactivation, whereas the BRI1 alphaC helix is moderately disordered and displays swinging behavior similar to numerous animal kinases. PMID: 28559283
    11. These results suggest that pleiotropic phenotypic alterations shown in the BAK1- overexpressing transgenic plants result from the constitutive activation of SA-mediated defense responses. PMID: 28153720
    12. Receptor like kinases (RLP)-mediated resistance and endocytosis require ligand-induced recruitment of BAK1/SERK3, reminiscent of BAK1/SERK3 interaction and subcellular fate of the FLAGELLIN SENSING 2 (FLS2) RLK. PMID: 26765243
    13. G protein signaling is directly activated by the pathogen-associated molecular pattern flagellin peptide 22 through its LRR RLK, FLS2, and co-receptor BAK1. PMID: 27235398
    14. BAK1 disruption stimulates the release of PROPEP3, produced in response to Pep application and during pathogen challenge, and renders PEPRs necessary for basal resistance. PMID: 26574534
    15. SERK1 or SERK2 extracellular domains are essential for SERK function in male sporogenesis, while the SERK3 extracellular and cytoplasmic domains are essential for SERK3 activity in brassinosteroid and flagellin signaling. PMID: 25864910
    16. Arabidopsis plants expressing FLS2 with the Q530A+Q627A double mutation were impaired both in detectable interaction with BAK1 and in FLS2-mediated responses, lending overall support to current models of FLS2 structure and function. PMID: 25356676
    17. Using MALDI-TOF MS, we identified Cys353, Cys374 and Cys408 as potential sites of glutathionylation on the BAK1 cytoplasmic domain and directed mutagenesis suggests that Cys353 and Cys408 are major sites of GRXC2-mediated glutathionylation. PMID: 25678081
    18. The substrate specificity of the signaling receptor-like kinases, CERK1 and BAK1, may determine the preference of downstream receptor-like cytoplasmic kinases (RLCKs). PMID: 24750441
    19. The results indicate that the extracellular domains of BRI1 and BAK1 interact with each other in a BL- and pH-dependent manner. PMID: 24126715
    20. A protein interaction surface on the C-terminal lobe of the kinase is identified and demonstrate that the isolated BRI1, SERK2 and SERK3 cytoplasmic segments form homodimers in solution and have a weak tendency to heteromerise. PMID: 24461462
    21. This work identifies PP2A as an important negative regulator of plant innate immunity that controls BAK1 activation in surface-localized immune receptor complexes. PMID: 25085430
    22. Detailed phenotypic analyses of bak1-SUP1 and a single mutant in which the bak1 mutation was segregated out (miR172-D) revealed that the overexpression of miR172 promotes leaf length elongation in adult plants and increases the root and hypocotyl growth during the seedling stage compared with that of wild type plants. PMID: 24732353
    23. Data indicate that the model included the contribution of the coreceptors SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE3 in BRASSINOSTEROID INSENSITIVE1 activities. PMID: 24072582
    24. BIK1 was found here to be autophosphorylated and transphosphorylated by BAK1 at multiple tyrosine residues to relay plant immune signaling. PMID: 24532660
    25. Colocalization of BRI1 and SERK3 in the plasma membrane is influenced by the brassinosteroid signaling status. PMID: 23796795
    26. BAK1-dependent PTI contributes to antiviral resistance in plants. PMID: 23902263
    27. these data reveal the molecular mechanisms underlying FLS2-BAK1 complex recognition of flg22 and provide insight into the immune receptor complex activation. PMID: 24114786
    28. BAK1 participates in the maintenance of the microbe-associated molecular patterns (MAMP)-specific response. PMID: 21668535
    29. bak1/elg-D promotes Arabidopsis growth by stimulating BR signaling at the expense of its readiness to respond to biotic stress factors PMID: 22961663
    30. Initial phosphorylation of the BAK1 activation loop locks BAK1 kinase in the active conformation. BAK1 phosphorylates and activates RLK, which phosphorylates downstream substrates for signal transduction and the development of innate immune response. PMID: 22547027
    31. crystal of BAK1 belonged to space group C2, with unit-cell parameters a = 70.3, b = 75.6, c = 71.9 A, beta = 93.1 degrees PMID: 22442239
    32. BON1 and BIR1 can be phosphorylated by BAK1 in vitro PMID: 21623975
    33. BAK1-dependent signaling pathways are differentially regulated by a novel allele of BAK1, bak1-5. PMID: 21593986
    34. several leucine-rich repeat receptor kinases form tight complexes with BAK1 almost instantaneously after ligand binding and the subsequent phosphorylation events are key initial steps in signal transduction PMID: 20103591
    35. We propose that the AtSERK3 protein is involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-AtSERK3 heterodimers. PMID: 15548744
    36. In vivo association of BRI1 and BAK1 was affected by endogenous and exogenous BR levels and that phosphorylation of both BRI1 and BAK1 on Thr residues was BR dependent. PMID: 15894717
    37. A previously identified photomorphogenesis mutant, elg, enhances high-light phototropism and represents a unique allele of BAK1/SERK3, a receptor kinase implicated in brassinosteroid perception. PMID: 16126860
    38. The effects of ectopic expression of BAK1 in transgenic rice on plant growth and plant growth regulators is reported. PMID: 17027118
    39. FLS2 and BAK1 form a complex in vivo, in a specific ligand-dependent manner, within the first minutes of stimulation with flagellin PMID: 17625569
    40. SERK3/BAK1 appears to integrate diverse perception events into downstream pathogen-associated molecular pattern responses, leading to immunity against a range of invading microbes. PMID: 17626179
    41. The paper shows that AvrPto and AvrPtoB bind the Arabidopsis receptor-like kinase BAK1, a shared signaling partner of both the flagellin receptor FLS2 and the brassinosteroid receptor BRI1. PMID: 18621007
    42. controls plant innate immunity, is the coreceptor of the flagellin receptor FLS2, and, together with SERK4, can mediate cell death control, all three in a brassinolide-independent fashion PMID: 18667726
    43. Brassinosteroids-dependent activation of BRI1 precedes association with BAK1 in planta, and that BRI1 positively regulates BAK1 phosphorylation levels in vivo. PMID: 18694562
    44. cytoplasmic domains of BRI1 and BAK1 also autophosphorylate on tyrosine residues and thus are dual-specificity kinases. PMID: 19124768

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Expressed ubiquitously.
  • 数据库链接：

    KEGG: ath:AT4G33430

    UniGene: At.376