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  4. Recombinant Arabidopsis thaliana Cyclin-dependent kinase F-1 (CDKF-1)

Recombinant Arabidopsis thaliana Cyclin-dependent kinase F-1 (CDKF-1)

  • 中文名称：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase F-1(CDKF-1),Yeast
  • 货号：
    CSB-YP529274DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase F-1(CDKF-1),Yeast
  • 货号：
    CSB-EP529274DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase F-1(CDKF-1),Yeast
  • 货号：
    CSB-EP529274DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase F-1(CDKF-1),Yeast
  • 货号：
    CSB-BP529274DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase F-1(CDKF-1),Yeast
  • 货号：
    CSB-MP529274DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CDKF-1
  • Uniprot No.：
  • 别名：
    CDKF-1; CAK1; At4g28980; F19B15.10Cyclin-dependent kinase F-1; CDKF;1; EC 2.7.11.22; EC 2.7.11.23; CDK-activating kinase 1-At; CAK1-At
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-479
  • 氨基酸序列
    MDKQPATSWS IHTRPEIIAK YEIFERVGSG AYADVYRARR LSDGLIVALK EIFDYQSAFR EIDALTILNG SPNVVVMHEY FWREEENAVL VLEFLRSDLA AVIRDGKRKK KVEGGDGFSV GEIKRWMIQI LTGVDACHRN LIVHRDLKPG NMLISDDGVL KLADFGQARI LMEHDIVASD ENQQAYKLED KDGETSEPPE VIPDYENSPR QGSDGQEREA MSKDEYFRQV EELKAKQVVR DDTDKDSNVH DGDISCLATC TVSEMDDDLG RNSFSYDADE AVDDTQGLMT SCVGTRWFRP PELLYGSTMY GLEVDLWSLG CVFAELLSLE PLFPGISDID QISRVTNVLG NLNEEVWPGC VDLPDYKSIS FAKVESPLGI EGCLPNHSGD VISLLKKLIC YDPASRATTM EMLNDKYLSE EPLPVPVSEL YVPPTMSGPD EDSPRKWNDY REMDSDSDFD GFGPMNVKPT SSGFTIEFP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    CDK-activating kinase that modulates CDKD-2 and CDKD-3 activities by phosphorylation of the T-loop. Activates CDKD-2 C-terminal domain (CTD) kinase activity. Activates CDKA-1 probably by phosphorylation. Posseses a CDK kinase activity independently of association with cyclin CYCH1-1. Phosphorylates the CTD of the large subunit of RNA polymerase II.
  • 基因功能参考文献：
    1. Ser phosphorylation of Arabidopsis thaliana RNAPII CTD is governed by CYCLIN-DEPENDENT KINASE F;1. PMID: 22547781
    2. CDKF;1 plays a major role in cyclin-dependent kinase and carboxy-terminal domain phosphorylation. PMID: 16856985
    3. CDKF;1 is dispensable for CDKA;1 activation but is essential for maintaining a steady-state level of CDKD;2. CDKF;1 mutants have severe defects in cell division, cell elongation and endoreduplication. PMID: 19368694
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 组织特异性：
    Highly expressed in suspension cell culture. Expressed at low levels in all plant organs.
  • 数据库链接：

    KEGG: ath:AT4G28980

    STRING: 3702.AT4G28980.1

    UniGene: At.19673