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  4. Recombinant Arabidopsis thaliana Cysteine protease ATG4b (ATG4B)

Recombinant Arabidopsis thaliana Cysteine protease ATG4b (ATG4B)

  • 货号：
    CSB-YP878671DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP878671DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP878671DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP878671DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP878671DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ATG4B; APG4B; At3g59950; F24G16.220; Cysteine protease ATG4b; EC 3.4.22.-; Autophagy-related protein 4 homolog b; AtAPG4b; Protein autophagy 4b
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-477
  • 氨基酸序列
    MKAICDRFVP SKCSSSSTSE KRDISSPTSL VSDSASSDNK SNLTLCSDVV ASSSPVSQLC REASTSGHNP VCTTHSSWTV ILKTASMASG AIRRFQDRVL GPSRTGISSS TSEIWLLGVC YKISEGESSE EADAGRVLAA FRQDFSSLIL MTYRRGFEPI GDTTYTSDVN WGCMLRSGQM LFAQALLFQR LGRSWRKKDS EPADEKYLEI LELFGDTEAS AFSIHNLILA GESYGLAAGS WVGPYAVCRS WESLARKNKE ETDDKHKSFS MAVHIVSGSE DGERGGAPIL CIEDVTKTCL EFSEGETEWP PILLLVPLVL GLDRVNPRYI PSLIATFTFP QSLGILGGKP GASTYIVGVQ EDKGFYLDPH DVQQVVTVKK ENQDVDTSSY HCNTLRYVPL ESLDPSLALG FYCQHKDDFD DFCIRATKLA GDSNGAPLFT VTQSHRRNDC GIAETSSSTE TSTEISGEEH EDDWQLL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cysteine protease required for autophagy, which is able to cleave the C-terminal part of ATG8 that may be subsequently converted to a smaller form, with a revealed C-terminal glycine. ATG8 is then activated by ATG7 and is subsequently attached to ATG3, another E2-like enzyme, after which the C-terminal glycine of ATG8 is conjugated to phosphatidylethanolamine by an amide bond. This conjugated form has the capacity for the binding to autophagosomes.
  • 基因功能参考文献：
    1. These results support the conclusion that CML24 affects autophagy progression through interactions with ATG4. PMID: 23039100
    2. AtATG4a is catalytically more active and has broad AtATG8 substrate specificity compared with AtATG4b. [ATG4b] PMID: 24658121
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C54 family
  • 组织特异性：
    Constitutively expressed.
  • 数据库链接：

    KEGG: ath:AT3G59950

    STRING: 3702.AT3G59950.1

    UniGene: At.1165